Click here to switch to the map view.

The map label for this gene is pilT-1 [H]

Identifier: 197119992

GI number: 197119992

Start: 4152823

End: 4153974

Strand: Direct

Name: pilT-1 [H]

Synonym: Gbem_3631

Alternate gene names: 197119992

Gene position: 4152823-4153974 (Clockwise)

Preceding gene: 197119991

Following gene: 197119993

Centisome position: 89.98

GC content: 62.93

Gene sequence:

>1152_bases
ATGGAACTTAACGAGATCCTCGGCATAGCCATGAAGGCGAAGGGGTCCGACATCCACCTGAAGGCGGGGCTTCCCCCCAT
CGTCAGGATCGACGGCGCCTTGCGCGCCATCCCCAACGCCGACCGGCTCTCGACGGACGCAGTCAGGACAATGGCGTTCA
ACATCATGAACGAACGCCAGAAACGGATCTTCGAGGAAAACTACGAGGTCGACCTGTCCTACGGTGTCCCGGGTCTCGGG
CGCTTCCGCGTCAACGTCTTCGCCCAGCGCGGCACGGTCGCCCTGGTTCTGCGCGCCATCCCGATCGCCATCCCGACGCT
CGAAACGCTGAACCTGCCGCCGGTGCTGAAAAAGCTGGCCCTGGAGCAGCGCGGCCTGATCCTCGTCACCGGCACCACGG
GGAGCGGCAAGTCCACCACGCTTGCCTCCATCATCGACTACATCAACGAGCACCGGACCTGCAACATCATCACCATCGAG
GATCCGGTCGAGTACCTGCACAAGGACAAGAAGAGCCTGATCAGCCAGCGCGAGGTCGGCTTCGACACCCTGACCTTCGG
CAAGGCGCTCACTTCGGCGCTACGCCAGGACCCGGACGTGATCCTCGTGGGCGAGATGCGCGACTACGAGACCATCGAGA
CGGCGCTCACCGCCGCCGAGACCGGCCACCTGGTCCTCTCCACCCTGCATACCCTGGACGCCGCCGAAACCGTCAACAGG
GTCATCTCCGTCTTCCCCCCTTACCACCAGAGGCAGGTGAGGATGCAGCTGTCGGGGATACTGAAAGGGGTCATCTCCCA
AAGGCTCGTGCCGCGCGCCGACGGCAAGGGTCGCGTCCCGGCGGTCGAGGTCCTGATCGGCACTGCGCGCATCAGGGATT
GCATCGACGACAAGGAGAAGACCAAGCAGATTCCCGAGGCGATCGCCCAGGGGTTCACGACCTACGGCATGCAGACCTTC
GACCAGTCGCTCATGCAGCTTATGACCAAGAACCTGATCAGCTACGAGGAAGCCATGAGGCAGTCGAGCAACCCCGACGA
CTTCGCCCTCAAGGTCTCCGGCATCTCTTCCACCTCCGACTCCAGCTGGGACGAGTTCACCAGCGAAGAGCCCTCCGCAG
ACGGCGGCGAGATGAATATCGAGAAGTACTAG

Upstream 100 bases:

>100_bases
CCGCCTGTTCCTGAAGGAGCACCCGGAGATCACCGCCGAAATCAGGGAAAAGCTGGTCAACCCCCAACAAGACGCCGCAA
CTCCCGGCGCAGCCTAAACC

Downstream 100 bases:

>100_bases
GTGCAGCGGGGAAGCGCCTTCGACTGCTGCCTGAGGGTGCTGGCCCTGCGCGACCACGCCGAGGCGGAACTGCGCAGGAA
ACTGGAAAGGAAGGGTTACC

Product: twitching motility pilus retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MELNEILGIAMKAKGSDIHLKAGLPPIVRIDGALRAIPNADRLSTDAVRTMAFNIMNERQKRIFEENYEVDLSYGVPGLG
RFRVNVFAQRGTVALVLRAIPIAIPTLETLNLPPVLKKLALEQRGLILVTGTTGSGKSTTLASIIDYINEHRTCNIITIE
DPVEYLHKDKKSLISQREVGFDTLTFGKALTSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLHTLDAAETVNR
VISVFPPYHQRQVRMQLSGILKGVISQRLVPRADGKGRVPAVEVLIGTARIRDCIDDKEKTKQIPEAIAQGFTTYGMQTF
DQSLMQLMTKNLISYEEAMRQSSNPDDFALKVSGISSTSDSSWDEFTSEEPSADGGEMNIEKY

Sequences:

>Translated_383_residues
MELNEILGIAMKAKGSDIHLKAGLPPIVRIDGALRAIPNADRLSTDAVRTMAFNIMNERQKRIFEENYEVDLSYGVPGLG
RFRVNVFAQRGTVALVLRAIPIAIPTLETLNLPPVLKKLALEQRGLILVTGTTGSGKSTTLASIIDYINEHRTCNIITIE
DPVEYLHKDKKSLISQREVGFDTLTFGKALTSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLHTLDAAETVNR
VISVFPPYHQRQVRMQLSGILKGVISQRLVPRADGKGRVPAVEVLIGTARIRDCIDDKEKTKQIPEAIAQGFTTYGMQTF
DQSLMQLMTKNLISYEEAMRQSSNPDDFALKVSGISSTSDSSWDEFTSEEPSADGGEMNIEKY
>Mature_383_residues
MELNEILGIAMKAKGSDIHLKAGLPPIVRIDGALRAIPNADRLSTDAVRTMAFNIMNERQKRIFEENYEVDLSYGVPGLG
RFRVNVFAQRGTVALVLRAIPIAIPTLETLNLPPVLKKLALEQRGLILVTGTTGSGKSTTLASIIDYINEHRTCNIITIE
DPVEYLHKDKKSLISQREVGFDTLTFGKALTSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLHTLDAAETVNR
VISVFPPYHQRQVRMQLSGILKGVISQRLVPRADGKGRVPAVEVLIGTARIRDCIDDKEKTKQIPEAIAQGFTTYGMQTF
DQSLMQLMTKNLISYEEAMRQSSNPDDFALKVSGISSTSDSSWDEFTSEEPSADGGEMNIEKY

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=324, Percent_Identity=45.0617283950617, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1789723, Length=285, Percent_Identity=36.4912280701754, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=38.2978723404255, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42292; Mature: 42292

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNEILGIAMKAKGSDIHLKAGLPPIVRIDGALRAIPNADRLSTDAVRTMAFNIMNERQ
CCHHHHHHHHEECCCCEEEEECCCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KRIFEENYEVDLSYGVPGLGRFRVNVFAQRGTVALVLRAIPIAIPTLETLNLPPVLKKLA
HHHHHCCCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LEQRGLILVTGTTGSGKSTTLASIIDYINEHRTCNIITIEDPVEYLHKDKKSLISQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTLTFGKALTSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLHTLDAAETVNR
CHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VISVFPPYHQRQVRMQLSGILKGVISQRLVPRADGKGRVPAVEVLIGTARIRDCIDDKEK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
TKQIPEAIAQGFTTYGMQTFDQSLMQLMTKNLISYEEAMRQSSNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SSWDEFTSEEPSADGGEMNIEKY
CCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MELNEILGIAMKAKGSDIHLKAGLPPIVRIDGALRAIPNADRLSTDAVRTMAFNIMNERQ
CCHHHHHHHHEECCCCEEEEECCCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KRIFEENYEVDLSYGVPGLGRFRVNVFAQRGTVALVLRAIPIAIPTLETLNLPPVLKKLA
HHHHHCCCEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LEQRGLILVTGTTGSGKSTTLASIIDYINEHRTCNIITIEDPVEYLHKDKKSLISQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTLTFGKALTSALRQDPDVILVGEMRDYETIETALTAAETGHLVLSTLHTLDAAETVNR
CHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VISVFPPYHQRQVRMQLSGILKGVISQRLVPRADGKGRVPAVEVLIGTARIRDCIDDKEK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHH
TKQIPEAIAQGFTTYGMQTFDQSLMQLMTKNLISYEEAMRQSSNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
SSWDEFTSEEPSADGGEMNIEKY
CCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]