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The map label for this gene is walK [H]

Identifier: 197119990

GI number: 197119990

Start: 4149492

End: 4151489

Strand: Direct

Name: walK [H]

Synonym: Gbem_3629

Alternate gene names: 197119990

Gene position: 4149492-4151489 (Clockwise)

Preceding gene: 197119989

Following gene: 197119991

Centisome position: 89.91

GC content: 63.16

Gene sequence:

>1998_bases
TTGCACTTCGCACCGTTCCTGCTCATAGCCGTTTGCGAGCTGATATTGCTCTTCATCGGGAGGCGCACCCCGCTGCCGGG
GTGGCTGCACCTTGGCGTCTTGGTCACGGTTACCGCCGTGCTCCTCTTTGTCGCCGCCCGGGCCTGGAAGCGGCAGCTCC
GCGTACAGGAAGGGCTGAAGCGCGAGTTGGAGGAGATGCGGCTGGAAGCGGTGAAAAGCGCCCGCCGCTACAAGAGCCTC
CTGGAGGGGGCGGGCAACGCCATCTTCATCTTCAGCGCCGACACCGGTCTTTTGGAGGAAGAAAACCGCGTGGGACGGGA
GCTTTTGGGTTTCAGCAAGGAGGAGCTCGACTCCATCCAGGCGCGCGACCTGTTGCATCCCGACGAGCATGAGCGGTTCC
GCTGCTTCCTCTACCAGGTGAAGCGCAACGGGCGCGGAGAACTGGACTCGGTCCGGATCAGGAAGAAAAACGGCACCTTC
TTCCTGGCCGAGATCAATGCCCGGCTCATCGACTTGGGGGACGAGCAGGTGGCGCACTGCCTCATGCGCGACATCACCAA
GAAAAGGCGCACCGAAAAAGAGATCTGGCAAAGAAACCGCGAGCTTTCCATCCTCAACGACATACTCACCGGCATGAACC
ATTCCGCCGACCTGGAACAGGAGCAGCAAAGAACCCTGTGGGAGATCATGGAGCTCTTCGACGCAGGGGGGGGCACCATG
CACCTGTACCGCGGCGACCAGGGAAGCGCCAGGCTTTGCGCCAGTTGCCACGTCTCTGCCGAATTGGAGCAGCGCCTCTC
CCAAAGCCTCGCCCAAGACCCGGAGCGCTTCCTGAAGGTGAAAAGGCTGAAGGCGCCGCAGATGAAGGAGTTGGGCGCCG
CCGCGGAGGGATGGCAATGCCTCACCTCGGTCCCCATCAGCGCGCAGGAGCACCTGGTCGGCGTAATGCACCTCATGCAT
CGCGACCCCTGCCGCTACAGCGCCGAAGAAATCCGCTTCCTGGAAAGCGTCGGCAAGCAGATGGGTAACATCATCGAGAA
AGGGAGGCTCTTCGCCGAGCTGAACTGGAAAAGCGGCGAACTGCTCCGCTCGCACCGCCTTTTGGAGAAGAGCAGCCACA
ACCTCTCCGTTTCCGAGATCCGGCTCAAGCAGAATCTGGCCTTGGTGGAGCAGGCTAGCCTGGAGCAGAACCGGCTGGAC
CGGATGAAGAACCAATTCCTGGGGATGGTCTCCCACGAGTTCAACACCCCGCTCACCAGCATCATCTCCGGCGTCGAGCA
TCTGCTGCAAAACGGCTGGCACAGCGAGCAGGAGGCGCGCTCCGTGCTGGAACTGGTGCGGGACGGCGGGCTCAGGCTGA
AGGGGCTGGTCGCCGACCTTTTGAAGCTGATCAAGGTGGAGGCCAGGCGTGGTGCGCTGGAGACCTCGGCGGTGCACCTG
CGGCATTTCCTGGACGAACTCGCGGCGCAGTTGCAGCCGCAGCTGGAAGAGCGGGGTCAGCGCGTCACCCTGGCGGGGTT
GGAAGAGCTCCCGTTCTTCGATGCGGACCGCAACTACCTGGAGCGCGTCTTCGGCGAGCTTTTGCAAAACGCCATCAGGT
TCAGCCCACACAAAGGGGAGATCCTGGTGACCGGGCGGGTGGTGGACCGCCCTGCCCTCCTGGAGCGCAGGGAAACCCTG
GAGCGCTTCAACCCCGAGTTCCTCAGGCGCTGCGGCGAACGCTGCTATCTGGAAGTGGAGGTGCGGGACAACGGCATCGG
CATCCCCCCCGAGGAGCAGCAGGGGATCTTCGGGATCTTCTACGAGGTCGGCGAGATAAGGCACCACTCCAGCGGCATGA
GCCGCGAGCAGGGAAGAGGGGCGGGGTTAGGTCTCGCCATGGTGAAGGGGATGGTGGAGGCCCACGGCGGGATGGTGTGG
GTGGAGAGTGCGGGAGGAAGCTCCTTCTTCCTGGTCCTTCCCCTGGAGCAGGAAGCGATCCAGCCGGCGCTGTTCTGA

Upstream 100 bases:

>100_bases
ACCGCGCTCGATTGGCTGCGCCGCTACCTCCTCACACGGAAGACGACACCAGGCCGGGGTTGACCGGTCACAACAAGTTG
CCGGTACAGAGGACACGTTC

Downstream 100 bases:

>100_bases
ACGGCCGAAAGGCCGTTCAACGTTCAACGTTCGACGTTTAAGATCGAGATTGAGATTGAGATTGAGATTGAGATTAAGTG
AAGGCGAACAGGCATGAACA

Product: PAS/GAF domain-containing heme-binding site containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MHFAPFLLIAVCELILLFIGRRTPLPGWLHLGVLVTVTAVLLFVAARAWKRQLRVQEGLKRELEEMRLEAVKSARRYKSL
LEGAGNAIFIFSADTGLLEEENRVGRELLGFSKEELDSIQARDLLHPDEHERFRCFLYQVKRNGRGELDSVRIRKKNGTF
FLAEINARLIDLGDEQVAHCLMRDITKKRRTEKEIWQRNRELSILNDILTGMNHSADLEQEQQRTLWEIMELFDAGGGTM
HLYRGDQGSARLCASCHVSAELEQRLSQSLAQDPERFLKVKRLKAPQMKELGAAAEGWQCLTSVPISAQEHLVGVMHLMH
RDPCRYSAEEIRFLESVGKQMGNIIEKGRLFAELNWKSGELLRSHRLLEKSSHNLSVSEIRLKQNLALVEQASLEQNRLD
RMKNQFLGMVSHEFNTPLTSIISGVEHLLQNGWHSEQEARSVLELVRDGGLRLKGLVADLLKLIKVEARRGALETSAVHL
RHFLDELAAQLQPQLEERGQRVTLAGLEELPFFDADRNYLERVFGELLQNAIRFSPHKGEILVTGRVVDRPALLERRETL
ERFNPEFLRRCGERCYLEVEVRDNGIGIPPEEQQGIFGIFYEVGEIRHHSSGMSREQGRGAGLGLAMVKGMVEAHGGMVW
VESAGGSSFFLVLPLEQEAIQPALF

Sequences:

>Translated_665_residues
MHFAPFLLIAVCELILLFIGRRTPLPGWLHLGVLVTVTAVLLFVAARAWKRQLRVQEGLKRELEEMRLEAVKSARRYKSL
LEGAGNAIFIFSADTGLLEEENRVGRELLGFSKEELDSIQARDLLHPDEHERFRCFLYQVKRNGRGELDSVRIRKKNGTF
FLAEINARLIDLGDEQVAHCLMRDITKKRRTEKEIWQRNRELSILNDILTGMNHSADLEQEQQRTLWEIMELFDAGGGTM
HLYRGDQGSARLCASCHVSAELEQRLSQSLAQDPERFLKVKRLKAPQMKELGAAAEGWQCLTSVPISAQEHLVGVMHLMH
RDPCRYSAEEIRFLESVGKQMGNIIEKGRLFAELNWKSGELLRSHRLLEKSSHNLSVSEIRLKQNLALVEQASLEQNRLD
RMKNQFLGMVSHEFNTPLTSIISGVEHLLQNGWHSEQEARSVLELVRDGGLRLKGLVADLLKLIKVEARRGALETSAVHL
RHFLDELAAQLQPQLEERGQRVTLAGLEELPFFDADRNYLERVFGELLQNAIRFSPHKGEILVTGRVVDRPALLERRETL
ERFNPEFLRRCGERCYLEVEVRDNGIGIPPEEQQGIFGIFYEVGEIRHHSSGMSREQGRGAGLGLAMVKGMVEAHGGMVW
VESAGGSSFFLVLPLEQEAIQPALF
>Mature_665_residues
MHFAPFLLIAVCELILLFIGRRTPLPGWLHLGVLVTVTAVLLFVAARAWKRQLRVQEGLKRELEEMRLEAVKSARRYKSL
LEGAGNAIFIFSADTGLLEEENRVGRELLGFSKEELDSIQARDLLHPDEHERFRCFLYQVKRNGRGELDSVRIRKKNGTF
FLAEINARLIDLGDEQVAHCLMRDITKKRRTEKEIWQRNRELSILNDILTGMNHSADLEQEQQRTLWEIMELFDAGGGTM
HLYRGDQGSARLCASCHVSAELEQRLSQSLAQDPERFLKVKRLKAPQMKELGAAAEGWQCLTSVPISAQEHLVGVMHLMH
RDPCRYSAEEIRFLESVGKQMGNIIEKGRLFAELNWKSGELLRSHRLLEKSSHNLSVSEIRLKQNLALVEQASLEQNRLD
RMKNQFLGMVSHEFNTPLTSIISGVEHLLQNGWHSEQEARSVLELVRDGGLRLKGLVADLLKLIKVEARRGALETSAVHL
RHFLDELAAQLQPQLEERGQRVTLAGLEELPFFDADRNYLERVFGELLQNAIRFSPHKGEILVTGRVVDRPALLERRETL
ERFNPEFLRRCGERCYLEVEVRDNGIGIPPEEQQGIFGIFYEVGEIRHHSSGMSREQGRGAGLGLAMVKGMVEAHGGMVW
VESAGGSSFFLVLPLEQEAIQPALF

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: COG5002

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=301, Percent_Identity=27.5747508305648, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=266, Percent_Identity=28.9473684210526, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786912, Length=430, Percent_Identity=25.1162790697674, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1790346, Length=297, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1786783, Length=260, Percent_Identity=27.3076923076923, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788713, Length=287, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=24.907063197026, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=341, Percent_Identity=26.3929618768328, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75652; Mature: 75652

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFAPFLLIAVCELILLFIGRRTPLPGWLHLGVLVTVTAVLLFVAARAWKRQLRVQEGLK
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELEEMRLEAVKSARRYKSLLEGAGNAIFIFSADTGLLEEENRVGRELLGFSKEELDSIQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHH
ARDLLHPDEHERFRCFLYQVKRNGRGELDSVRIRKKNGTFFLAEINARLIDLGDEQVAHC
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCHHEECCHHHHHHH
LMRDITKKRRTEKEIWQRNRELSILNDILTGMNHSADLEQEQQRTLWEIMELFDAGGGTM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
HLYRGDQGSARLCASCHVSAELEQRLSQSLAQDPERFLKVKRLKAPQMKELGAAAEGWQC
EEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCCCCCHH
LTSVPISAQEHLVGVMHLMHRDPCRYSAEEIRFLESVGKQMGNIIEKGRLFAELNWKSGE
HHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
LLRSHRLLEKSSHNLSVSEIRLKQNLALVEQASLEQNRLDRMKNQFLGMVSHEFNTPLTS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IISGVEHLLQNGWHSEQEARSVLELVRDGGLRLKGLVADLLKLIKVEARRGALETSAVHL
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RHFLDELAAQLQPQLEERGQRVTLAGLEELPFFDADRNYLERVFGELLQNAIRFSPHKGE
HHHHHHHHHHHCHHHHHCCCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ILVTGRVVDRPALLERRETLERFNPEFLRRCGERCYLEVEVRDNGIGIPPEEQQGIFGIF
EEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHH
YEVGEIRHHSSGMSREQGRGAGLGLAMVKGMVEAHGGMVWVESAGGSSFFLVLPLEQEAI
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCHHHC
QPALF
CCCCC
>Mature Secondary Structure
MHFAPFLLIAVCELILLFIGRRTPLPGWLHLGVLVTVTAVLLFVAARAWKRQLRVQEGLK
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELEEMRLEAVKSARRYKSLLEGAGNAIFIFSADTGLLEEENRVGRELLGFSKEELDSIQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHH
ARDLLHPDEHERFRCFLYQVKRNGRGELDSVRIRKKNGTFFLAEINARLIDLGDEQVAHC
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCHHEECCHHHHHHH
LMRDITKKRRTEKEIWQRNRELSILNDILTGMNHSADLEQEQQRTLWEIMELFDAGGGTM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
HLYRGDQGSARLCASCHVSAELEQRLSQSLAQDPERFLKVKRLKAPQMKELGAAAEGWQC
EEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCCCCCHH
LTSVPISAQEHLVGVMHLMHRDPCRYSAEEIRFLESVGKQMGNIIEKGRLFAELNWKSGE
HHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
LLRSHRLLEKSSHNLSVSEIRLKQNLALVEQASLEQNRLDRMKNQFLGMVSHEFNTPLTS
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IISGVEHLLQNGWHSEQEARSVLELVRDGGLRLKGLVADLLKLIKVEARRGALETSAVHL
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RHFLDELAAQLQPQLEERGQRVTLAGLEELPFFDADRNYLERVFGELLQNAIRFSPHKGE
HHHHHHHHHHHCHHHHHCCCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
ILVTGRVVDRPALLERRETLERFNPEFLRRCGERCYLEVEVRDNGIGIPPEEQQGIFGIF
EEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHH
YEVGEIRHHSSGMSREQGRGAGLGLAMVKGMVEAHGGMVWVESAGGSSFFLVLPLEQEAI
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCHHHC
QPALF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA