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The map label for this gene is cspR [H]

Identifier: 197119687

GI number: 197119687

Start: 3819638

End: 3820114

Strand: Direct

Name: cspR [H]

Synonym: Gbem_3322

Alternate gene names: 197119687

Gene position: 3819638-3820114 (Clockwise)

Preceding gene: 197119682

Following gene: 197119688

Centisome position: 82.76

GC content: 57.65

Gene sequence:

>477_bases
ATGTCAGAGATGAAACCTTTTCACATAGTGCTCGTCGAGCCGGAGATACCGCCTAACACTGGCAACATCGCCAGGCTCTG
CGGAGCAACCGGTACCGTACTGCACCTGGTGGGAAAACTTGGTTTCTCGACGGACGACCGCTACCTCAAAAGGGCGGGGC
TCGATTACTGGAGCGAAGTGGAGATTCATTACTGGCCCGACCTCCCGGCACTAAAGCAGGCCTATCCCGGCTCAAGGTTC
GTCTATACCAGCAAGAAGGCGGAGAAAAGCTACCTGGCCTTCACTTTCCTTTACGGCGACTTCATCGTCTTCGGCAAAGA
GACCAAGGGGCTTCCCGAAGAACTGATCGAGGCGAACGCGCAGACGTCGGTCCGCATCCCCATTCCCGGCAAGGTCCGCA
GCCTCAACCTCTCCACCTCGGCCGGGATCGTTCTTTACGAGGCCTTGCGTCAGACCGGTGCAGTCGAAGGTGCATGA

Upstream 100 bases:

>100_bases
TCTCATTCCGTCTCCAAATTGGGCAATATTTGCTGTTGAGTTTGCCCCTGGTTTCGTCTGCTGAGAAAGCGATGAACTGC
ACTGTCATAGAGGGAGCGTG

Downstream 100 bases:

>100_bases
AAAGGAGTCCGCATGTTTGCAAACGGCAGCGAAGAAGGTTACCTGGAAGTGCTTGAAGGTATCCGGCGCAAGACGCTGGT
CTACGGCGATGAAACCCTGA

Product: SpoU-class rRNA methyltransferase CspR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSEMKPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWPDLPALKQAYPGSRF
VYTSKKAEKSYLAFTFLYGDFIVFGKETKGLPEELIEANAQTSVRIPIPGKVRSLNLSTSAGIVLYEALRQTGAVEGA

Sequences:

>Translated_158_residues
MSEMKPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWPDLPALKQAYPGSRF
VYTSKKAEKSYLAFTFLYGDFIVFGKETKGLPEELIEANAQTSVRIPIPGKVRSLNLSTSAGIVLYEALRQTGAVEGA
>Mature_157_residues
SEMKPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWPDLPALKQAYPGSRFV
YTSKKAEKSYLAFTFLYGDFIVFGKETKGLPEELIEANAQTSVRIPIPGKVRSLNLSTSAGIVLYEALRQTGAVEGA

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=43.2432432432432, Blast_Score=127, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17452; Mature: 17321

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMKPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEE
EIHYWPDLPALKQAYPGSRFVYTSKKAEKSYLAFTFLYGDFIVFGKETKGLPEELIEANA
EEEECCCCCHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHCCC
QTSVRIPIPGKVRSLNLSTSAGIVLYEALRQTGAVEGA
CCEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEMKPFHIVLVEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEE
EIHYWPDLPALKQAYPGSRFVYTSKKAEKSYLAFTFLYGDFIVFGKETKGLPEELIEANA
EEEECCCCCHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEECCCCCCCCHHHHHCCC
QTSVRIPIPGKVRSLNLSTSAGIVLYEALRQTGAVEGA
CCEEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]