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The map label for this gene is mdeA [H]

Identifier: 197119654

GI number: 197119654

Start: 3781948

End: 3783231

Strand: Direct

Name: mdeA [H]

Synonym: Gbem_3288

Alternate gene names: 197119654

Gene position: 3781948-3783231 (Clockwise)

Preceding gene: 197119647

Following gene: 197119657

Centisome position: 81.95

GC content: 63.71

Gene sequence:

>1284_bases
ATGAAAAAGGATTGGCGCATCGAGACCCAGGCGATCCAGGAAGGCTTCGCCCCCAAGGACGGAGATCCCCGGATCCTGCC
GATCTACCAGAGCACGACCTTCAAGTTCTCCAGCGCGGAGCACGTGGCGAAACTGTTCGACCTCGAGGTGGGCGGACACT
TCTACACCCGGCTCAGCAACCCGACCGCCGAGGGTTTCGAGACCAAGATAGCGGCCATGGAAGGGGGCGTCGCCGCCATG
GCCACCTCCAGCGGACAGGCCGCGACCAGCATGGCCATCATGAACATCTGCCAGGCCGGCCAGCACGTGGTCGCCGCCAG
CACCTTGTACGGCGGCACCTACTCGCTTTTCGCCAACACCTTCCCGAAGATGGGCATCGAGGTGACCTTCGTCGATCCCG
AGGCAGACGAGGCCGCCATCGAGGCCGCCTTCCGCCCCGAGACCCGCGCACTTTTCGGCGAGACCATAGGGAATCCAGGC
TTGAACGTGCTCGACTTCGAGAAGTTCTCCCGGATCGCCAAGAAGATGCAGGTGCCGCTCATCATCGACAACACCTTCCC
GACGCCGTACCTGTGCCGCCCCTTCGAGCACGGCGCGGACATAGTTATCCACTCGGCAACCAAGTACATCGACGGGCACG
CCACCAGCGTGGGCGGGGTCATCATAGACAGCGGCAATTTCAACTGGGGCAACGGCAAGTACCCCGAGATGACGGAGCCG
GACTCCAGCTACCACGGGCTCAAGTACCTGGAGACCTTCGGCAAGCTCGCCTACATCGTCAAGGCGCGGGTGCAGCTCAT
GCGCGACCTGGGCTCCTGTCCTGCGCCGATGAACGCCTTCCTGTTCAACCTGGGGCTGGAGACGCTGCCGCTGCGCATGC
AGCGCCACAGCGAGAACGCGCTCGCCATGGCCAAGTACCTGGAAAAGCACCCAGCGGTGAGCTGGGTGACCTACCCGGGA
CTGGAGAGCCACAAAAGCTACGCCCGCAGCAAGAAGTACCTCCCCAAGGGAGCGAGCGGCGTCCTCACCTTCGGCATCAA
GGGAGGGGCCGCGGCAGGAAAGAAATTCATGGAGAGCTGCCGGCTCGTGGCGCTGGTGGTGCACGTAGGGGACGCCAGGA
GCTGCGTCCTGCACCCGGCGAGCACCACCCACCGCCAGCTGAACGAGGAGCAGCAGATAGCTTCCGGCGTCTCCCCCGAC
CTGATCAGGCTTTCGGTCGGCATCGAGCACATCGACGACCTGATCGCGGACGTGAACCAGGCGCTTTTGGCCAGCCAGAA
GTAA

Upstream 100 bases:

>100_bases
CCTCGGGCTGCGGCTGCCTGAATATTTCCCTGCAAATCGCGGGGCAATCTGCTATGATGCCGCCCTGCATTTGGGCATCC
GATTTTGACAGGAGGGATAC

Downstream 100 bases:

>100_bases
AAGCGCGACGCTACCGGCCTTTGGCCGCGCTGCCAACAAAGAAGGGGCGAGTGAATCCACTCGCCCCTTCTCATTTTTAA
AGCTTCCGCTACCCTACTTG

Product: O-acetyl-L-homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKKDWRIETQAIQEGFAPKDGDPRILPIYQSTTFKFSSAEHVAKLFDLEVGGHFYTRLSNPTAEGFETKIAAMEGGVAAM
ATSSGQAATSMAIMNICQAGQHVVAASTLYGGTYSLFANTFPKMGIEVTFVDPEADEAAIEAAFRPETRALFGETIGNPG
LNVLDFEKFSRIAKKMQVPLIIDNTFPTPYLCRPFEHGADIVIHSATKYIDGHATSVGGVIIDSGNFNWGNGKYPEMTEP
DSSYHGLKYLETFGKLAYIVKARVQLMRDLGSCPAPMNAFLFNLGLETLPLRMQRHSENALAMAKYLEKHPAVSWVTYPG
LESHKSYARSKKYLPKGASGVLTFGIKGGAAAGKKFMESCRLVALVVHVGDARSCVLHPASTTHRQLNEEQQIASGVSPD
LIRLSVGIEHIDDLIADVNQALLASQK

Sequences:

>Translated_427_residues
MKKDWRIETQAIQEGFAPKDGDPRILPIYQSTTFKFSSAEHVAKLFDLEVGGHFYTRLSNPTAEGFETKIAAMEGGVAAM
ATSSGQAATSMAIMNICQAGQHVVAASTLYGGTYSLFANTFPKMGIEVTFVDPEADEAAIEAAFRPETRALFGETIGNPG
LNVLDFEKFSRIAKKMQVPLIIDNTFPTPYLCRPFEHGADIVIHSATKYIDGHATSVGGVIIDSGNFNWGNGKYPEMTEP
DSSYHGLKYLETFGKLAYIVKARVQLMRDLGSCPAPMNAFLFNLGLETLPLRMQRHSENALAMAKYLEKHPAVSWVTYPG
LESHKSYARSKKYLPKGASGVLTFGIKGGAAAGKKFMESCRLVALVVHVGDARSCVLHPASTTHRQLNEEQQIASGVSPD
LIRLSVGIEHIDDLIADVNQALLASQK
>Mature_427_residues
MKKDWRIETQAIQEGFAPKDGDPRILPIYQSTTFKFSSAEHVAKLFDLEVGGHFYTRLSNPTAEGFETKIAAMEGGVAAM
ATSSGQAATSMAIMNICQAGQHVVAASTLYGGTYSLFANTFPKMGIEVTFVDPEADEAAIEAAFRPETRALFGETIGNPG
LNVLDFEKFSRIAKKMQVPLIIDNTFPTPYLCRPFEHGADIVIHSATKYIDGHATSVGGVIIDSGNFNWGNGKYPEMTEP
DSSYHGLKYLETFGKLAYIVKARVQLMRDLGSCPAPMNAFLFNLGLETLPLRMQRHSENALAMAKYLEKHPAVSWVTYPG
LESHKSYARSKKYLPKGASGVLTFGIKGGAAAGKKFMESCRLVALVVHVGDARSCVLHPASTTHRQLNEEQQIASGVSPD
LIRLSVGIEHIDDLIADVNQALLASQK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=34.1232227488152, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=31.5165876777251, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=30.4038004750594, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1790375, Length=404, Percent_Identity=30.6930693069307, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1789383, Length=432, Percent_Identity=24.3055555555556, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=406, Percent_Identity=33.9901477832512, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=36.1904761904762, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=34.8448687350835, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=46.6819221967963, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=34.6511627906977, Blast_Score=215, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=25.5656108597285, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=345, Percent_Identity=26.6666666666667, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=33.4117647058824, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46469; Mature: 46469

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDWRIETQAIQEGFAPKDGDPRILPIYQSTTFKFSSAEHVAKLFDLEVGGHFYTRLSN
CCCCCEEHHHHHHCCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHCCCCEEEEECCC
PTAEGFETKIAAMEGGVAAMATSSGQAATSMAIMNICQAGQHVVAASTLYGGTYSLFANT
CCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHC
FPKMGIEVTFVDPEADEAAIEAAFRPETRALFGETIGNPGLNVLDFEKFSRIAKKMQVPL
CCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCCE
IIDNTFPTPYLCRPFEHGADIVIHSATKYIDGHATSVGGVIIDSGNFNWGNGKYPEMTEP
EEECCCCCCCEECCCCCCCCEEEECCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
DSSYHGLKYLETFGKLAYIVKARVQLMRDLGSCPAPMNAFLFNLGLETLPLRMQRHSENA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHCCCHH
LAMAKYLEKHPAVSWVTYPGLESHKSYARSKKYLPKGASGVLTFGIKGGAAAGKKFMESC
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
RLVALVVHVGDARSCVLHPASTTHRQLNEEQQIASGVSPDLIRLSVGIEHIDDLIADVNQ
HHEEEEEEECCCCEEEECCCCHHHHHCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
ALLASQK
HHHHCCC
>Mature Secondary Structure
MKKDWRIETQAIQEGFAPKDGDPRILPIYQSTTFKFSSAEHVAKLFDLEVGGHFYTRLSN
CCCCCEEHHHHHHCCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHCCCCEEEEECCC
PTAEGFETKIAAMEGGVAAMATSSGQAATSMAIMNICQAGQHVVAASTLYGGTYSLFANT
CCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHC
FPKMGIEVTFVDPEADEAAIEAAFRPETRALFGETIGNPGLNVLDFEKFSRIAKKMQVPL
CCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCCCE
IIDNTFPTPYLCRPFEHGADIVIHSATKYIDGHATSVGGVIIDSGNFNWGNGKYPEMTEP
EEECCCCCCCEECCCCCCCCEEEECCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
DSSYHGLKYLETFGKLAYIVKARVQLMRDLGSCPAPMNAFLFNLGLETLPLRMQRHSENA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHCCCHH
LAMAKYLEKHPAVSWVTYPGLESHKSYARSKKYLPKGASGVLTFGIKGGAAAGKKFMESC
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHH
RLVALVVHVGDARSCVLHPASTTHRQLNEEQQIASGVSPDLIRLSVGIEHIDDLIADVNQ
HHEEEEEEECCCCEEEECCCCHHHHHCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHH
ALLASQK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]