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The map label for this gene is tnpR [H]

Identifier: 197118126

GI number: 197118126

Start: 2006364

End: 2007050

Strand: Direct

Name: tnpR [H]

Synonym: Gbem_1741

Alternate gene names: 197118126

Gene position: 2006364-2007050 (Clockwise)

Preceding gene: 197118125

Following gene: 197118127

Centisome position: 43.47

GC content: 56.33

Gene sequence:

>687_bases
ATGGCTACAGGGAAGCTCATTTCATACCTCAGGGTGTCAACCGACAAGCAGGGGAAGGCTGGACTAGGGATAGAAGCGCA
GAGGGCAAGTATCACCAGCTACCTCAACGGTGGAACCTGGGAGTTGCTGCAGGAGTATGTGGAGGTGGAGTCGGGGAAGA
CCTCTGACAGGCCACAGCTGCAGGAAGCTCTCAAGGCATGCAAGAGGACAGGAGCTGCTCTGGTCATCGCCAAGCTGGAT
AGGTTGAGCCGCGACCCTGACTTCCTCGGTACGCTGATGAAGAGTGATATCGAGTTCATCGCCTGTGACATGCCCGATGC
CAACAAGTTTATGGTACGGATCATGGCGGCACTGGCAGAGAAGGAACGGGAGATGATTTCCGAAAGGACCAAGGCGGCAC
TCAAGGCTGCGAAGACCAGAGGAGTCAAGCTCGGCAAGCCAGAAAATGCAACCCCAGAGGGGAGAGCTAAAGGTATCGCT
CAGAGCCTGGAGAAGAGGCAAGAGAAAGCCAGTCGGTTCACTGCTGAGGTTCTGCCGCTAATCCTGGAGCACAAAGCGCA
GGGTCAGAGCCTCAGGGGGATTGCAGACGTATTGAACTGTTCCAGCGTCCTGACCGCAAGCGGAAAGAGTGGTGCGTGGA
CAGCCTCTGCCGTGAAGGTCATCCTGGACAGGTCAACACTGCCATGA

Upstream 100 bases:

>100_bases
CCTATCACCCATCACCATGCAGTATCTCTTGACATCCTCTGTCTATCGTGTATGTTTTGGTCGAACGTCCATTCAAGCAA
AACGTACGGAGGTCCACAGC

Downstream 100 bases:

>100_bases
TTCCGAGTACCTGGAGATAACTCTTCGCTTTGGTACAAGAACGGCAAGGGCATGTGGCTAGGCCTCCAGGTTCCGGACGC
AGGTAGCAAGATGTCTCTTC

Product: resolvase-like serine recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MATGKLISYLRVSTDKQGKAGLGIEAQRASITSYLNGGTWELLQEYVEVESGKTSDRPQLQEALKACKRTGAALVIAKLD
RLSRDPDFLGTLMKSDIEFIACDMPDANKFMVRIMAALAEKEREMISERTKAALKAAKTRGVKLGKPENATPEGRAKGIA
QSLEKRQEKASRFTAEVLPLILEHKAQGQSLRGIADVLNCSSVLTASGKSGAWTASAVKVILDRSTLP

Sequences:

>Translated_228_residues
MATGKLISYLRVSTDKQGKAGLGIEAQRASITSYLNGGTWELLQEYVEVESGKTSDRPQLQEALKACKRTGAALVIAKLD
RLSRDPDFLGTLMKSDIEFIACDMPDANKFMVRIMAALAEKEREMISERTKAALKAAKTRGVKLGKPENATPEGRAKGIA
QSLEKRQEKASRFTAEVLPLILEHKAQGQSLRGIADVLNCSSVLTASGKSGAWTASAVKVILDRSTLP
>Mature_227_residues
ATGKLISYLRVSTDKQGKAGLGIEAQRASITSYLNGGTWELLQEYVEVESGKTSDRPQLQEALKACKRTGAALVIAKLDR
LSRDPDFLGTLMKSDIEFIACDMPDANKFMVRIMAALAEKEREMISERTKAALKAAKTRGVKLGKPENATPEGRAKGIAQ
SLEKRQEKASRFTAEVLPLILEHKAQGQSLRGIADVLNCSSVLTASGKSGAWTASAVKVILDRSTLP

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=158, Percent_Identity=36.7088607594937, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 24664; Mature: 24533

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGKLISYLRVSTDKQGKAGLGIEAQRASITSYLNGGTWELLQEYVEVESGKTSDRPQL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
QEALKACKRTGAALVIAKLDRLSRDPDFLGTLMKSDIEFIACDMPDANKFMVRIMAALAE
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
KEREMISERTKAALKAAKTRGVKLGKPENATPEGRAKGIAQSLEKRQEKASRFTAEVLPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEHKAQGQSLRGIADVLNCSSVLTASGKSGAWTASAVKVILDRSTLP
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATGKLISYLRVSTDKQGKAGLGIEAQRASITSYLNGGTWELLQEYVEVESGKTSDRPQL
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
QEALKACKRTGAALVIAKLDRLSRDPDFLGTLMKSDIEFIACDMPDANKFMVRIMAALAE
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
KEREMISERTKAALKAAKTRGVKLGKPENATPEGRAKGIAQSLEKRQEKASRFTAEVLPL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEHKAQGQSLRGIADVLNCSSVLTASGKSGAWTASAVKVILDRSTLP
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2997130 [H]