Click here to switch to the map view.

The map label for this gene is nifH2 [H]

Identifier: 197118116

GI number: 197118116

Start: 1994920

End: 1995744

Strand: Direct

Name: nifH2 [H]

Synonym: Gbem_1731

Alternate gene names: 197118116

Gene position: 1994920-1995744 (Clockwise)

Preceding gene: 197118115

Following gene: 197118117

Centisome position: 43.23

GC content: 66.42

Gene sequence:

>825_bases
ATGGCTGTGGAACATCTGGTTATCGCAGGCAAGAGCGGAGTGGGGAAGTCGACCACCGCGGCCAATCTGAGCGCCGCCCT
GGCGGAGAAGGGCAAGCGGGTGCTCCTCATCGGATACGACAGCCGCTGGAGTTCGACCGGCATCCTGTGCGGCGGCAACG
AGCTGACCCCGCTGCCGGGATGGCAGGGAGAGGATGCGCCGCTCCAGGCGCTGGGGCACGCGGGGGCGCTCTGCCTGGAA
GCGGGGGCGGCGCTGGAGTCCGGAGCCGCGACCGGGGGTGAGATCCTCACCCATCCGCTCATCGCCGACTACGACCCGGA
GTACGTGGTGCACGACACCGCCTGGGAGCCGGCAGGGAGCTTCACGCTCCCGCCGGGAATCGACGGCGTCTCCCGCCTTC
TGGCCGTGACCTCGGCGGACAAGTGCGCGCTGCAGGCGGTGAACGACATCTTCGCCTGGCTCAACACCGTGGCCTCGGCC
GAGTGCCGCTTCGGCGGGGTGGTCGCCAACAACCTCTCTGGGCCGCTGTACGAGGCGATAGTCTCCGACTTCGCCAGCCA
GACGGGAGCCGTCATTTCGGCCACCGTTCCCCGCTCCATCACGGTCTCCGTGAGCGACTTCTACAGCCAGACGCTGCTGC
AGTCGGCCCCCTTTTCCCACGTTTCCTATGCCTACCGGAAATTGGCCCGGGACTTGGAGAAAAGCGGGGAGAAGCGCCGC
CCGAAACCGCTTAGTAGTGACGCGCTGTCCAACTGGGCGCAGAAATGGGGGGACATAATCACCGAGTTGGAGACGGGGGT
GGTTAAGGATGGACTCGGAATCTAG

Upstream 100 bases:

>100_bases
ATTATCCTGATCGGGCTGGCCGTCGAGAACCTGCTCTTCGCGGGGATCGAGCGACGGACGGTACTACGCTGGGGAATGAA
GCACTGAAAGGAGGTGCGCC

Downstream 100 bases:

>100_bases
CCCGGATGCTTATGATTAAAACCGTTGACTTCCTGCAGTTATCAAAGTACCTCCTTGGCAAATTCTCATAATCCATTCCA
CGCGAGACACCAATGAATAT

Product: iron-sulfur ATPase, NifH/FrxC family

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAVEHLVIAGKSGVGKSTTAANLSAALAEKGKRVLLIGYDSRWSSTGILCGGNELTPLPGWQGEDAPLQALGHAGALCLE
AGAALESGAATGGEILTHPLIADYDPEYVVHDTAWEPAGSFTLPPGIDGVSRLLAVTSADKCALQAVNDIFAWLNTVASA
ECRFGGVVANNLSGPLYEAIVSDFASQTGAVISATVPRSITVSVSDFYSQTLLQSAPFSHVSYAYRKLARDLEKSGEKRR
PKPLSSDALSNWAQKWGDIITELETGVVKDGLGI

Sequences:

>Translated_274_residues
MAVEHLVIAGKSGVGKSTTAANLSAALAEKGKRVLLIGYDSRWSSTGILCGGNELTPLPGWQGEDAPLQALGHAGALCLE
AGAALESGAATGGEILTHPLIADYDPEYVVHDTAWEPAGSFTLPPGIDGVSRLLAVTSADKCALQAVNDIFAWLNTVASA
ECRFGGVVANNLSGPLYEAIVSDFASQTGAVISATVPRSITVSVSDFYSQTLLQSAPFSHVSYAYRKLARDLEKSGEKRR
PKPLSSDALSNWAQKWGDIITELETGVVKDGLGI
>Mature_273_residues
AVEHLVIAGKSGVGKSTTAANLSAALAEKGKRVLLIGYDSRWSSTGILCGGNELTPLPGWQGEDAPLQALGHAGALCLEA
GAALESGAATGGEILTHPLIADYDPEYVVHDTAWEPAGSFTLPPGIDGVSRLLAVTSADKCALQAVNDIFAWLNTVASAE
CRFGGVVANNLSGPLYEAIVSDFASQTGAVISATVPRSITVSVSDFYSQTLLQSAPFSHVSYAYRKLARDLEKSGEKRRP
KPLSSDALSNWAQKWGDIITELETGVVKDGLGI

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 28598; Mature: 28467

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEHLVIAGKSGVGKSTTAANLSAALAEKGKRVLLIGYDSRWSSTGILCGGNELTPLPG
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCCC
WQGEDAPLQALGHAGALCLEAGAALESGAATGGEILTHPLIADYDPEYVVHDTAWEPAGS
CCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCC
FTLPPGIDGVSRLLAVTSADKCALQAVNDIFAWLNTVASAECRFGGVVANNLSGPLYEAI
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHH
VSDFASQTGAVISATVPRSITVSVSDFYSQTLLQSAPFSHVSYAYRKLARDLEKSGEKRR
HHHHHHHCCCEEEEECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
PKPLSSDALSNWAQKWGDIITELETGVVKDGLGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVEHLVIAGKSGVGKSTTAANLSAALAEKGKRVLLIGYDSRWSSTGILCGGNELTPLPG
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCCCCC
WQGEDAPLQALGHAGALCLEAGAALESGAATGGEILTHPLIADYDPEYVVHDTAWEPAGS
CCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCC
FTLPPGIDGVSRLLAVTSADKCALQAVNDIFAWLNTVASAECRFGGVVANNLSGPLYEAI
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHH
VSDFASQTGAVISATVPRSITVSVSDFYSQTLLQSAPFSHVSYAYRKLARDLEKSGEKRR
HHHHHHHCCCEEEEECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
PKPLSSDALSNWAQKWGDIITELETGVVKDGLGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA