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The map label for this gene is lysS [H]

Identifier: 197117228

GI number: 197117228

Start: 988677

End: 990155

Strand: Direct

Name: lysS [H]

Synonym: Gbem_0836

Alternate gene names: 197117228

Gene position: 988677-990155 (Clockwise)

Preceding gene: 197117227

Following gene: 197117229

Centisome position: 21.42

GC content: 62.47

Gene sequence:

>1479_bases
ATGGAAGAGTTGAGCGAACTGCTGCTGCAGAGAAGACGCAAGGTAGATGCATTGTGGGAGGCGGGTATCAACCCGTACCC
GAACGATTACCGGCCGCAACACACCTCGGCCGACGTCCGCGCCGCTTACGGCGACAAGGAAGTCATCGAGGAAGAGCCGC
AGAGCTTCGTGGTGGCCGGCCGCATATTGATGCGCCGCTCCTTCGGCAAGGCCGCCTTCGTACAGGTGCAGGACCGCAAA
GGGCGCATGCAGCTCTACATGAAGAAGGACACCCTGGGCGAGGAGCTCTTCGCCGAGTTCGAAAACTACGACATAGGCGA
CATCATCGGCGCCACCGGCACCCCTTTCCGCACCAAGACCGGGGAGCTTTCCCTCGCCGTCAGCGAACTGCGCCTGCTGA
CCAAGTCGCTCTTGCCGCTTCCCGAGAAGTTCCACGGACTTACCGACGTAGAGACCCGTTACCGCCAGCGCTACGTCGAC
CTGATCGTCTCCCCCGACGTGCGCGAGGTGTTCTACAAGCGCTCCCGCGTCATCCAGCTCATCCGCGACTTCATGACCAA
GAACGACTTCCTGGAAGTGGAAACCCCGATGATGCATCCCATCCCCGGCGGCGCCACGGCGAAGCCGTTCGCGACGCACC
ATAACGCGCTGGACATGGAGCTCTTCCTGCGCATCGCCCCCGAGCTCTACCTGAAGAGGCTCGTGGTCGGCGGCTTCGAG
CGCGTCTTCGAGATCAACCGCAACTTCAGAAACGAAGGGATCTCGGTGCGCCACAACCCCGAGTTCACCATGATGGAGTT
CTACCAGGCCTACGCCACCTTCGAGGACCTGATGAACTACACCGAGGAACTCCTCTGCCACGTGGCCCAGGAACTTCTCG
GCACCCTCGACTTCAGCTACGGCGGCGAGCCGATCAGCTTCCAGCGCCCCTGGCGCCGCTTCACCGTGAAGGAGGCGATC
CTCGAGTACGGCGACATCGACGCGAAGAGCCTTGAGGACCGCGACTTAGCCTACGCCTACGCCCAGAAGATCGGGCTGGA
ACTCCCCGAGGACGTGGGGTACGGCAAGCTCATCACCGAGATCTTCGAGGAGGTCGCCGAGATCAAGCTGATCCAGCCGA
CCTTCATCACCAACTACCCGACCGAGGTTTCGCCCCTTTCCAGAAAGAGCGACCAGGACCCCGAGTACGTGGACCGCTTC
GAGTTCTTCTGCGCCGGGCGCGAAATGGCCAACGCCTTCTCCGAGCTGAACGACCCGCGCGACCAGAAGGAGCGCTTCCT
GGCCCAGGTGGCCGCCAAGGACAAGGGGGACGAGGAGGCGCACTACATGGACGAGGACTACATCCGCGCCCTGGAGTACG
GGCTTCCTCCCACCGCCGGCGAGGGGATCGGCATCGACCGCCTGGTCATGCTCTTGACCGACTCCCCGTCCATCCGCGAC
GTCATCCTGTTCCCGCAGTTAAGGAAAGAGGCGAAATAG

Upstream 100 bases:

>100_bases
GTCCTATTCCCCTGTTTTCCCTTGACTTAGAGTTGCCAATTAGTTTAGTTTGACGGATTGTTTTCACCCCCCAAGGCCAA
CAAGAAAGGTTGCAGATTAC

Downstream 100 bases:

>100_bases
ATGCCCTTCGAGCTCTTCATAGGGCTGCGCTACCTTAAGGCCAAGCGCAAATCGACCTTCATCTCCCTGATCACCCTGAT
CTCCGTGGCAGGGGTCGCCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYRPQHTSADVRAAYGDKEVIEEEPQSFVVAGRILMRRSFGKAAFVQVQDRK
GRMQLYMKKDTLGEELFAEFENYDIGDIIGATGTPFRTKTGELSLAVSELRLLTKSLLPLPEKFHGLTDVETRYRQRYVD
LIVSPDVREVFYKRSRVIQLIRDFMTKNDFLEVETPMMHPIPGGATAKPFATHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMNYTEELLCHVAQELLGTLDFSYGGEPISFQRPWRRFTVKEAI
LEYGDIDAKSLEDRDLAYAYAQKIGLELPEDVGYGKLITEIFEEVAEIKLIQPTFITNYPTEVSPLSRKSDQDPEYVDRF
EFFCAGREMANAFSELNDPRDQKERFLAQVAAKDKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEAK

Sequences:

>Translated_492_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYRPQHTSADVRAAYGDKEVIEEEPQSFVVAGRILMRRSFGKAAFVQVQDRK
GRMQLYMKKDTLGEELFAEFENYDIGDIIGATGTPFRTKTGELSLAVSELRLLTKSLLPLPEKFHGLTDVETRYRQRYVD
LIVSPDVREVFYKRSRVIQLIRDFMTKNDFLEVETPMMHPIPGGATAKPFATHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMNYTEELLCHVAQELLGTLDFSYGGEPISFQRPWRRFTVKEAI
LEYGDIDAKSLEDRDLAYAYAQKIGLELPEDVGYGKLITEIFEEVAEIKLIQPTFITNYPTEVSPLSRKSDQDPEYVDRF
EFFCAGREMANAFSELNDPRDQKERFLAQVAAKDKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEAK
>Mature_492_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYRPQHTSADVRAAYGDKEVIEEEPQSFVVAGRILMRRSFGKAAFVQVQDRK
GRMQLYMKKDTLGEELFAEFENYDIGDIIGATGTPFRTKTGELSLAVSELRLLTKSLLPLPEKFHGLTDVETRYRQRYVD
LIVSPDVREVFYKRSRVIQLIRDFMTKNDFLEVETPMMHPIPGGATAKPFATHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMNYTEELLCHVAQELLGTLDFSYGGEPISFQRPWRRFTVKEAI
LEYGDIDAKSLEDRDLAYAYAQKIGLELPEDVGYGKLITEIFEEVAEIKLIQPTFITNYPTEVSPLSRKSDQDPEYVDRF
EFFCAGREMANAFSELNDPRDQKERFLAQVAAKDKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEAK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=41.2825651302605, Blast_Score=359, Evalue=4e-99,
Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=41.2825651302605, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI45439306, Length=353, Percent_Identity=24.0793201133144, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=58.3673469387755, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=57.3469387755102, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=32.398753894081, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788173, Length=296, Percent_Identity=27.7027027027027, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=499, Percent_Identity=42.2845691382766, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17535927, Length=499, Percent_Identity=42.2845691382766, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71994340, Length=499, Percent_Identity=42.2845691382766, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17551876, Length=471, Percent_Identity=22.7176220806794, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=276, Percent_Identity=25.3623188405797, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=490, Percent_Identity=42.8571428571429, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324256, Length=481, Percent_Identity=30.5613305613306, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=26.1363636363636, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321807, Length=457, Percent_Identity=23.8512035010941, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=41.6837782340862, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640851, Length=487, Percent_Identity=41.6837782340862, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=460, Percent_Identity=22.3913043478261, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24584738, Length=285, Percent_Identity=25.9649122807018, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56811; Mature: 56811

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDYRPQHTSADVRAAYGDKEVIEEEPQSFVVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHCCHHHHHHH
RILMRRSFGKAAFVQVQDRKGRMQLYMKKDTLGEELFAEFENYDIGDIIGATGTPFRTKT
HHHHHHHCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GELSLAVSELRLLTKSLLPLPEKFHGLTDVETRYRQRYVDLIVSPDVREVFYKRSRVIQL
CCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDFMTKNDFLEVETPMMHPIPGGATAKPFATHHNALDMELFLRIAPELYLKRLVVGGFE
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMNYTEELLCHVAQELLGTLDFSY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGEPISFQRPWRRFTVKEAILEYGDIDAKSLEDRDLAYAYAQKIGLELPEDVGYGKLITE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAEIKLIQPTFITNYPTEVSPLSRKSDQDPEYVDRFEFFCAGREMANAFSELNDPR
HHHHHHHEEEECCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
DQKERFLAQVAAKDKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEAK
EEECHHHHHCCC
>Mature Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDYRPQHTSADVRAAYGDKEVIEEEPQSFVVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHCCHHHHHHH
RILMRRSFGKAAFVQVQDRKGRMQLYMKKDTLGEELFAEFENYDIGDIIGATGTPFRTKT
HHHHHHHCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GELSLAVSELRLLTKSLLPLPEKFHGLTDVETRYRQRYVDLIVSPDVREVFYKRSRVIQL
CCHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDFMTKNDFLEVETPMMHPIPGGATAKPFATHHNALDMELFLRIAPELYLKRLVVGGFE
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMNYTEELLCHVAQELLGTLDFSY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGEPISFQRPWRRFTVKEAILEYGDIDAKSLEDRDLAYAYAQKIGLELPEDVGYGKLITE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAEIKLIQPTFITNYPTEVSPLSRKSDQDPEYVDRFEFFCAGREMANAFSELNDPR
HHHHHHHEEEECCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCH
DQKERFLAQVAAKDKGDEEAHYMDEDYIRALEYGLPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEAK
EEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA