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The map label for this gene is glgX [H]

Identifier: 197117214

GI number: 197117214

Start: 973354

End: 975474

Strand: Direct

Name: glgX [H]

Synonym: Gbem_0822

Alternate gene names: 197117214

Gene position: 973354-975474 (Clockwise)

Preceding gene: 197117213

Following gene: 197117215

Centisome position: 21.09

GC content: 64.64

Gene sequence:

>2121_bases
GTGAAAGTTACGGCAAAAACAAGCAAGAAAAACGCTCCGGCGTACCCCAAGGGGAACACCTCGCCGCTCGGCGCCACCGT
GTCGCACGGAGGCGTCAACTTCAGCGTCTTCGCCAGGGACTGCACCGGAGTCGAACTCCTGCTCTTCGATGCTGCCGACG
ACGCCATTCCGTCCCGCGTCATCACCCTCGACCCGCAGCAAAACCGCACCTACCATTACTGGCACGTCTTCGTCCCCGGC
ATAGGAGAAGGGCAGCTTTACGGCTACCGGGTGGCCGGCCCCTTCGAGCCGCAGCGCGGGCGCAGGTTCGATCCCGGCAA
GGTCCTGATCGATCCCTACGGCCGCGCCGTCGCGGTCCCCAAGGGGTACTGCAGGGGCGACGCCTGCCTCCCCGGGGACA
ACGCCGCGACCGCCATGAAGAGCGTCGTCGCCGACCCGCGCGACTACGACTGGGAGGGGGATCTTCCCCTCAAGCGCCCT
TACTCCAACACCGTCATCTACGAGATGCACGTGGCCGGCTTCACCAAGGACCCTTCCTCCGGGGTCTCGACCGACAAGCG
CGGCACCTACGCCGGGCTCGTGGAGAAGATCCCCTACTTAAAGGACCTGGGGGTCACCGCGGTGGAACTGCTGCCGGTGT
TCCAATTCGACCCCCACGACGCCCCCTTCGGGCTGGTCAATTACTGGGGGTACAGCCCCATCTCCTTCTTCGCCCCCCAT
GCCGGCTTCAGTTCGCGCACCGAACCGCTGGGACCTCTGGACGAGTTCCGCGACATGGTGAAGGCGCTGCACAAGGCGGG
AATAGAGGTGATCCTCGACGTGGTTTACAACCACACCTCCGAGGGGGACCACAAGGGACCTACCTTCTGCTACCGCGGCT
TCGCCAACGACGTCTACTATTCGCTTGCCCCCGACGGGAGCTACATCAACCACACCGGCTGCGGCAACACCATGAACGCG
AACCACCACGTGGTGCGCCGGCTCATCATCGACAGCCTGCACTACTGGGTCAAGGAGATGCACGTCGACGGCTTCCGTTT
CGATCTTGCCTCCATCCTCTCCCGCGACGGCCAGGGGCGCCCGCTCAAGAACCCCCCGATCCTCTGGGACATCGAGTCGG
ACCCGGCGCTGGCGGGGATCAAGCTGATCGCCGAGGCGTGGGACGCAGGCGGGCTCTACCAGGTGGGGAGCTTCATCGGC
GACAGCTGGAAGGAGTGGAACGGGGAGTTCCGCGACGACGTGCGCCGCTTCCTCAAAGGGGACGAAGGGGTGGTATCCCG
TTTCGCGGCAAGGATGCTGGCGAGCCCGGACATCTACGGGCACCAGGAGCGCGAACCGGAGCAGAGCATCAACTTCGTCA
CCTGCCACGACGGCTTTACCCTGAACGACCTGGTCTCCTACAACAAAAAGCACAACGAGGCCAACGGCGAGTCCAACAGG
GACGGCTCCGACGCCAACCTGAGCTGGAACTGCGGCGTCGAGGGGCCTGCCGGCGACCCGGCGGTCGAGGAACTGCGCAA
CCGGCAGGTCAAGAACTTCATGGCGGTGACGCTTTTAGCCCTTGGTACCCCGATGATCCTGATGGGGGACGAGATGCGGC
GCAGCCAGCAGGGTAACAACAACGCCTACTGCCAGGACAACCGCATCGGCTGGTTCGATTGGAGCCTCCACGAGCGCCAC
GCCGACATTTACCGCTTCACCAAGGAGATGATCAAGGCAAGGCTGATGCAGACCGGCACACTGGAGGACGCGCGGACCCT
GAGCCAGTTGCTGGGGCAGGCGCGCCTGGAATGGCACGGGGTCAACCTCGGGACCCCCGACTGGAGCCACGAGTCGCACA
GCATTGCCCTGACGGTCTGGAGCCGCTCCCGCCACGTTGTGTTCCATTACCTGGTCAATGCATACTGGGAACCGCTGACC
TTCACGCTTCCACCCCCGCGCAGGCTTGCCGGCGGCACCTGGTACCGCTGGATCGACACCTCTCTTCCTTCGCCCGACGA
CATCGTTCCCTGGGACCTTACCCCGGCGCATGCCTCGGGAAGCTACCAGCTCCCCCCGCGCAGCCTCGTGGTGCTGATCG
CTAAGGCGCGCAGTGCCAAAGGGCGCTCCGCTTCCGACTGA

Upstream 100 bases:

>100_bases
GACTATAGTGTCACAAGGCAAAAAAGGCATCGTCAATTCAAGTGCGGTGGCGACTGTGACTGCACCGGCCGCCCCTGAAA
GCTCGAAAGAAGGATCTACG

Downstream 100 bases:

>100_bases
GTTCACCCCCCTTGTCCCCATCCGCTTGCCATTACCTGCAAGGAGATCAGCATGTCCTCTACCTTTGGCACTCTCTTCAG
GGTATCCACCTTTGGCGAAA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MKVTAKTSKKNAPAYPKGNTSPLGATVSHGGVNFSVFARDCTGVELLLFDAADDAIPSRVITLDPQQNRTYHYWHVFVPG
IGEGQLYGYRVAGPFEPQRGRRFDPGKVLIDPYGRAVAVPKGYCRGDACLPGDNAATAMKSVVADPRDYDWEGDLPLKRP
YSNTVIYEMHVAGFTKDPSSGVSTDKRGTYAGLVEKIPYLKDLGVTAVELLPVFQFDPHDAPFGLVNYWGYSPISFFAPH
AGFSSRTEPLGPLDEFRDMVKALHKAGIEVILDVVYNHTSEGDHKGPTFCYRGFANDVYYSLAPDGSYINHTGCGNTMNA
NHHVVRRLIIDSLHYWVKEMHVDGFRFDLASILSRDGQGRPLKNPPILWDIESDPALAGIKLIAEAWDAGGLYQVGSFIG
DSWKEWNGEFRDDVRRFLKGDEGVVSRFAARMLASPDIYGHQEREPEQSINFVTCHDGFTLNDLVSYNKKHNEANGESNR
DGSDANLSWNCGVEGPAGDPAVEELRNRQVKNFMAVTLLALGTPMILMGDEMRRSQQGNNNAYCQDNRIGWFDWSLHERH
ADIYRFTKEMIKARLMQTGTLEDARTLSQLLGQARLEWHGVNLGTPDWSHESHSIALTVWSRSRHVVFHYLVNAYWEPLT
FTLPPPRRLAGGTWYRWIDTSLPSPDDIVPWDLTPAHASGSYQLPPRSLVVLIAKARSAKGRSASD

Sequences:

>Translated_706_residues
MKVTAKTSKKNAPAYPKGNTSPLGATVSHGGVNFSVFARDCTGVELLLFDAADDAIPSRVITLDPQQNRTYHYWHVFVPG
IGEGQLYGYRVAGPFEPQRGRRFDPGKVLIDPYGRAVAVPKGYCRGDACLPGDNAATAMKSVVADPRDYDWEGDLPLKRP
YSNTVIYEMHVAGFTKDPSSGVSTDKRGTYAGLVEKIPYLKDLGVTAVELLPVFQFDPHDAPFGLVNYWGYSPISFFAPH
AGFSSRTEPLGPLDEFRDMVKALHKAGIEVILDVVYNHTSEGDHKGPTFCYRGFANDVYYSLAPDGSYINHTGCGNTMNA
NHHVVRRLIIDSLHYWVKEMHVDGFRFDLASILSRDGQGRPLKNPPILWDIESDPALAGIKLIAEAWDAGGLYQVGSFIG
DSWKEWNGEFRDDVRRFLKGDEGVVSRFAARMLASPDIYGHQEREPEQSINFVTCHDGFTLNDLVSYNKKHNEANGESNR
DGSDANLSWNCGVEGPAGDPAVEELRNRQVKNFMAVTLLALGTPMILMGDEMRRSQQGNNNAYCQDNRIGWFDWSLHERH
ADIYRFTKEMIKARLMQTGTLEDARTLSQLLGQARLEWHGVNLGTPDWSHESHSIALTVWSRSRHVVFHYLVNAYWEPLT
FTLPPPRRLAGGTWYRWIDTSLPSPDDIVPWDLTPAHASGSYQLPPRSLVVLIAKARSAKGRSASD
>Mature_706_residues
MKVTAKTSKKNAPAYPKGNTSPLGATVSHGGVNFSVFARDCTGVELLLFDAADDAIPSRVITLDPQQNRTYHYWHVFVPG
IGEGQLYGYRVAGPFEPQRGRRFDPGKVLIDPYGRAVAVPKGYCRGDACLPGDNAATAMKSVVADPRDYDWEGDLPLKRP
YSNTVIYEMHVAGFTKDPSSGVSTDKRGTYAGLVEKIPYLKDLGVTAVELLPVFQFDPHDAPFGLVNYWGYSPISFFAPH
AGFSSRTEPLGPLDEFRDMVKALHKAGIEVILDVVYNHTSEGDHKGPTFCYRGFANDVYYSLAPDGSYINHTGCGNTMNA
NHHVVRRLIIDSLHYWVKEMHVDGFRFDLASILSRDGQGRPLKNPPILWDIESDPALAGIKLIAEAWDAGGLYQVGSFIG
DSWKEWNGEFRDDVRRFLKGDEGVVSRFAARMLASPDIYGHQEREPEQSINFVTCHDGFTLNDLVSYNKKHNEANGESNR
DGSDANLSWNCGVEGPAGDPAVEELRNRQVKNFMAVTLLALGTPMILMGDEMRRSQQGNNNAYCQDNRIGWFDWSLHERH
ADIYRFTKEMIKARLMQTGTLEDARTLSQLLGQARLEWHGVNLGTPDWSHESHSIALTVWSRSRHVVFHYLVNAYWEPLT
FTLPPPRRLAGGTWYRWIDTSLPSPDDIVPWDLTPAHASGSYQLPPRSLVVLIAKARSAKGRSASD

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=647, Percent_Identity=42.1947449768161, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789839, Length=285, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78752; Mature: 78752

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTAKTSKKNAPAYPKGNTSPLGATVSHGGVNFSVFARDCTGVELLLFDAADDAIPSRV
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCEEEEEEECCCCCCCCEE
ITLDPQQNRTYHYWHVFVPGIGEGQLYGYRVAGPFEPQRGRRFDPGKVLIDPYGRAVAVP
EEECCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECC
KGYCRGDACLPGDNAATAMKSVVADPRDYDWEGDLPLKRPYSNTVIYEMHVAGFTKDPSS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GVSTDKRGTYAGLVEKIPYLKDLGVTAVELLPVFQFDPHDAPFGLVNYWGYSPISFFAPH
CCCCCCCCHHHHHHHHCCHHHHCCCCHHHHEEEEECCCCCCCCCHHHHCCCCCHHHHCCC
AGFSSRTEPLGPLDEFRDMVKALHKAGIEVILDVVYNHTSEGDHKGPTFCYRGFANDVYY
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEE
SLAPDGSYINHTGCGNTMNANHHVVRRLIIDSLHYWVKEMHVDGFRFDLASILSRDGQGR
EECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
PLKNPPILWDIESDPALAGIKLIAEAWDAGGLYQVGSFIGDSWKEWNGEFRDDVRRFLKG
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHCC
DEGVVSRFAARMLASPDIYGHQEREPEQSINFVTCHDGFTLNDLVSYNKKHNEANGESNR
CHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
DGSDANLSWNCGVEGPAGDPAVEELRNRQVKNFMAVTLLALGTPMILMGDEMRRSQQGNN
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCC
NAYCQDNRIGWFDWSLHERHADIYRFTKEMIKARLMQTGTLEDARTLSQLLGQARLEWHG
CCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEC
VNLGTPDWSHESHSIALTVWSRSRHVVFHYLVNAYWEPLTFTLPPPRRLAGGTWYRWIDT
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEEEC
SLPSPDDIVPWDLTPAHASGSYQLPPRSLVVLIAKARSAKGRSASD
CCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKVTAKTSKKNAPAYPKGNTSPLGATVSHGGVNFSVFARDCTGVELLLFDAADDAIPSRV
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCEEEEEEECCCCCCCCEE
ITLDPQQNRTYHYWHVFVPGIGEGQLYGYRVAGPFEPQRGRRFDPGKVLIDPYGRAVAVP
EEECCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECC
KGYCRGDACLPGDNAATAMKSVVADPRDYDWEGDLPLKRPYSNTVIYEMHVAGFTKDPSS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GVSTDKRGTYAGLVEKIPYLKDLGVTAVELLPVFQFDPHDAPFGLVNYWGYSPISFFAPH
CCCCCCCCHHHHHHHHCCHHHHCCCCHHHHEEEEECCCCCCCCCHHHHCCCCCHHHHCCC
AGFSSRTEPLGPLDEFRDMVKALHKAGIEVILDVVYNHTSEGDHKGPTFCYRGFANDVYY
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEE
SLAPDGSYINHTGCGNTMNANHHVVRRLIIDSLHYWVKEMHVDGFRFDLASILSRDGQGR
EECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
PLKNPPILWDIESDPALAGIKLIAEAWDAGGLYQVGSFIGDSWKEWNGEFRDDVRRFLKG
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHCC
DEGVVSRFAARMLASPDIYGHQEREPEQSINFVTCHDGFTLNDLVSYNKKHNEANGESNR
CHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
DGSDANLSWNCGVEGPAGDPAVEELRNRQVKNFMAVTLLALGTPMILMGDEMRRSQQGNN
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCC
NAYCQDNRIGWFDWSLHERHADIYRFTKEMIKARLMQTGTLEDARTLSQLLGQARLEWHG
CCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEC
VNLGTPDWSHESHSIALTVWSRSRHVVFHYLVNAYWEPLTFTLPPPRRLAGGTWYRWIDT
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCCEEEEEEC
SLPSPDDIVPWDLTPAHASGSYQLPPRSLVVLIAKARSAKGRSASD
CCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]