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The map label for this gene is cspLA [H]

Identifier: 197117042

GI number: 197117042

Start: 767516

End: 767716

Strand: Direct

Name: cspLA [H]

Synonym: Gbem_0645

Alternate gene names: 197117042

Gene position: 767516-767716 (Clockwise)

Preceding gene: 197117041

Following gene: 197117043

Centisome position: 16.63

GC content: 58.21

Gene sequence:

>201_bases
ATGGCAAACGGTGTGGTGAAATGGTTCAACGACGCTAAGGGATTCGGCTTCATCGAGCAGGATAACGGGGTGGACGTGTT
CGTGCACTTCTCGTCGATCCAGGGAGACGGTTTCAAGTCCCTCGTCGAGGGTGACTCGGTCACCTTTGACGTCGTGCAGG
GCGCCAAGGGGCCGCAGGCGGCCAACGTGGTAAAGAACTAG

Upstream 100 bases:

>100_bases
CGTGTAATAGGGCAGGCAGCAGATGCGTCGAAGACGCACAAAGGGGGGGGCGGCGCAGTCGCCCCCCATCCAACAACAAG
CAGCAAAGGGAGAAAAAGTA

Downstream 100 bases:

>100_bases
CTAAGCCTATCCACCATCATTGGGCCCCGGATTTCCGGGGCTCTTTTTGTTTTCCCCGCCGCACTGCCGCCGCGGGGCGC
GGAGTACCTCCAGATGCGCA

Product: cold shock DNA/RNA-binding protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 65

Protein sequence:

>66_residues
MANGVVKWFNDAKGFGFIEQDNGVDVFVHFSSIQGDGFKSLVEGDSVTFDVVQGAKGPQAANVVKN

Sequences:

>Translated_66_residues
MANGVVKWFNDAKGFGFIEQDNGVDVFVHFSSIQGDGFKSLVEGDSVTFDVVQGAKGPQAANVVKN
>Mature_65_residues
ANGVVKWFNDAKGFGFIEQDNGVDVFVHFSSIQGDGFKSLVEGDSVTFDVVQGAKGPQAANVVKN

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=62.9032258064516, Blast_Score=82, Evalue=8e-18,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=63.4920634920635, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=60.3174603174603, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=61.2903225806452, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1787107, Length=65, Percent_Identity=55.3846153846154, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=58.0645161290323, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=55.5555555555556, Blast_Score=69, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7021; Mature: 6890

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGVVKWFNDAKGFGFIEQDNGVDVFVHFSSIQGDGFKSLVEGDSVTFDVVQGAKGPQA
CCCCEEEECCCCCCCCEEECCCCEEEEEEEECCCCCCCCHHHCCCCEEEEEECCCCCCCC
ANVVKN
CCCCCC
>Mature Secondary Structure 
ANGVVKWFNDAKGFGFIEQDNGVDVFVHFSSIQGDGFKSLVEGDSVTFDVVQGAKGPQA
CCCEEEECCCCCCCCEEECCCCEEEEEEEECCCCCCCCHHHCCCCEEEEEECCCCCCCC
ANVVKN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]