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The map label for this gene is rpoH [H]

Identifier: 197116970

GI number: 197116970

Start: 696093

End: 696950

Strand: Direct

Name: rpoH [H]

Synonym: Gbem_0573

Alternate gene names: 197116970

Gene position: 696093-696950 (Clockwise)

Preceding gene: 197116968

Following gene: 197116975

Centisome position: 15.08

GC content: 59.91

Gene sequence:

>858_bases
ATGACACACAGCATGCCGGTACCTATCGACAACCTGACCCTTTACCTCGCGGAAATCAACCGTTTTCCGCTGCTTACGGC
GGACGAGGAAATGGATTTGGCTGGCAGGTTCCGTCGCGAGGGAGACCTTGAGGCGGCGCACCGGCTGATCACCGCGAACC
TTCGTTTCGTGGTGAAAATAGCAGGCGAATACCGTTCCTACGGGATGAAGATGCTCGACCTGATCCAGGAAGGGAATATC
GGGCTGATGATGGCGGTGAGAAAGTTCGACCCTGACAAGGGGGTGCGGCTCATCTCCTACGCGGTCTGGTGGATTAGGGC
CTACATCCAGAACCACATCATCTCCGCCTGGAGCCTGTTAAAGATCGGGACCACGCAGGCGCAGAGAAAGCTCTTCTTTA
AGCTGAACCAGGCGAAGCAGGCGATCGTCAACATGACCGGCGAGGGCGACCTGCACGCGGCGGCGATATCGCTGGACGTG
AAGGACAACGAGGCCCGGGAGATGGAACAGCGCCTCCGGGGCGAATTGTCGCTCGACGTCGAGATGTTCGAAGGCGAGGG
AGGGGGGACCTTCCTGGAGAGCCTTCCCGACCTGCGGCAGAACCAGGAGGAGATGCTGGCCGAGCGCCAGGAGACCACCA
TCCTAGAGCGAAACGTAGCCAGCGCGCTGGAAAACCTGAACGAGAAAGAGAAGTTCGTCATCGAGCAGCGCGTAGCCGCG
GAGGACCCGATGACCCTGCAGGAAATAGCCGATCACTTCTCCATCTCGCGCGAGCGGGTCCGCCAGATAGAAGAGGGTGC
GCTCAAGAAGATGAAGCGCTTCCTCGCCCCGCTGGTGATGGAAGCCGAGGCGGCGTAA

Upstream 100 bases:

>100_bases
CTTGATTTTTTACATGCTGCTTGATATATTGTTAGCGCTCACGAGAGCGGAGTGCTAACAGCAGGAGGCAAAACCTGCAC
TCACAGGGGGTAACAGTACT

Downstream 100 bases:

>100_bases
CCGAAGCTGCCGCTGATACAACAAAATTTAAAAGGGCGCCTCCGGAAATGGAAGCGCCCTTTTTTTATGCTGCATTCTTT
ACTTACGAAGGTTGGTTCCC

Product: RNA polymerase sigma-32 factor RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTHSMPVPIDNLTLYLAEINRFPLLTADEEMDLAGRFRREGDLEAAHRLITANLRFVVKIAGEYRSYGMKMLDLIQEGNI
GLMMAVRKFDPDKGVRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQRKLFFKLNQAKQAIVNMTGEGDLHAAAISLDV
KDNEAREMEQRLRGELSLDVEMFEGEGGGTFLESLPDLRQNQEEMLAERQETTILERNVASALENLNEKEKFVIEQRVAA
EDPMTLQEIADHFSISRERVRQIEEGALKKMKRFLAPLVMEAEAA

Sequences:

>Translated_285_residues
MTHSMPVPIDNLTLYLAEINRFPLLTADEEMDLAGRFRREGDLEAAHRLITANLRFVVKIAGEYRSYGMKMLDLIQEGNI
GLMMAVRKFDPDKGVRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQRKLFFKLNQAKQAIVNMTGEGDLHAAAISLDV
KDNEAREMEQRLRGELSLDVEMFEGEGGGTFLESLPDLRQNQEEMLAERQETTILERNVASALENLNEKEKFVIEQRVAA
EDPMTLQEIADHFSISRERVRQIEEGALKKMKRFLAPLVMEAEAA
>Mature_284_residues
THSMPVPIDNLTLYLAEINRFPLLTADEEMDLAGRFRREGDLEAAHRLITANLRFVVKIAGEYRSYGMKMLDLIQEGNIG
LMMAVRKFDPDKGVRLISYAVWWIRAYIQNHIISAWSLLKIGTTQAQRKLFFKLNQAKQAIVNMTGEGDLHAAAISLDVK
DNEAREMEQRLRGELSLDVEMFEGEGGGTFLESLPDLRQNQEEMLAERQETTILERNVASALENLNEKEKFVIEQRVAAE
DPMTLQEIADHFSISRERVRQIEEGALKKMKRFLAPLVMEAEAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is essential for normal fruiting body formation [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=39.6428571428571, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789098, Length=278, Percent_Identity=32.3741007194245, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=30.4721030042918, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32539; Mature: 32408

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSMPVPIDNLTLYLAEINRFPLLTADEEMDLAGRFRREGDLEAAHRLITANLRFVVKI
CCCCCCCCHHHHHEEEEHHCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHEEEEEE
AGEYRSYGMKMLDLIQEGNIGLMMAVRKFDPDKGVRLISYAVWWIRAYIQNHIISAWSLL
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQRKLFFKLNQAKQAIVNMTGEGDLHAAAISLDVKDNEAREMEQRLRGELSLDV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCEEEE
EMFEGEGGGTFLESLPDLRQNQEEMLAERQETTILERNVASALENLNEKEKFVIEQRVAA
EEEECCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
EDPMTLQEIADHFSISRERVRQIEEGALKKMKRFLAPLVMEAEAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
THSMPVPIDNLTLYLAEINRFPLLTADEEMDLAGRFRREGDLEAAHRLITANLRFVVKI
CCCCCCCHHHHHEEEEHHCCCCEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHEEEEEE
AGEYRSYGMKMLDLIQEGNIGLMMAVRKFDPDKGVRLISYAVWWIRAYIQNHIISAWSLL
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGTTQAQRKLFFKLNQAKQAIVNMTGEGDLHAAAISLDVKDNEAREMEQRLRGELSLDV
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCEEEE
EMFEGEGGGTFLESLPDLRQNQEEMLAERQETTILERNVASALENLNEKEKFVIEQRVAA
EEEECCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
EDPMTLQEIADHFSISRERVRQIEEGALKKMKRFLAPLVMEAEAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501037 [H]