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The map label for this gene is fixL [H]

Identifier: 197116933

GI number: 197116933

Start: 645801

End: 647465

Strand: Reverse

Name: fixL [H]

Synonym: Gbem_0535

Alternate gene names: 197116933

Gene position: 647465-645801 (Counterclockwise)

Preceding gene: 197116934

Following gene: 197116932

Centisome position: 14.03

GC content: 60.72

Gene sequence:

>1665_bases
ATGTGGGTTTTGGCCTCGTTCTTCCCCCTGGCGGCGTTGGCGCTGCAGTGGCTTTTGTGGGAAAGACTGCGCCCCTATGT
CTGGCTCTTCTTCTACCCCGCAGTATTCCTAAGCTCCTGGGCCGGGGGAAGGGTAGCGGGGCTCCTGGCTACAGCTTTCT
CCGCGACGGTCGTCTGGTATTTCTTCATCCCCCCCGAACAAAGTTTCTCGCTGGAGCACCCCTCGACAGCAATCGCGCTG
CTCATCTTCGCCGGCATGGGCATTCTTTTCTCGCTGTTCCACGAACGACTCAGAAGAGCGGACCGACAGATCCGGCTGAC
GCTGGCGGAGGCGACCTCGGGGCGCGAGCAACTTGAACGGCGGATGGAGGAGCGCACCAAAGAACTGGTCGATCTGGTCG
ACGAGCTGCGACGCAAGGAGACGGACCTGCGGAGATCCCAAGAGCTGCTGAAACTTTTCATCGAGTACGCGCCGGTGCCG
CTTGCCATGTTCGACAGCGAGATGCGTTACCTCTACGCGAGCCGGCGCTGGCGCAGCGACTACGGCCTGGGAAACCGCCC
CCTCGTCAAGGTCAGCCACTACGAAATATTCCCGGAGATCCCCCAGCACTGGAAGGAGCTGCACCAGCGCGGGCTCGCCG
GAGAGATCCTGCGCGAGGAGGCCGAAGAATTCCGGCGCGCCGACGGCACGGTCCAGTGGCTGCGCTGGGAACTCCGTCCC
TGGTACGACGCTGGGGGAATGGTGGGGGGAATCGTCATCTTCAGCGAGGACATCACCAACAGAAAGTGCGCCGAGGACGC
GCTGCAAAAACTCAACGAGGAGCTAGAACTGCGCGTGGTCCAGCGTACCGAGACACTAGACCAGATCCTGGGCGAGCGGG
AGGCGCAGAATGCGGAGCTGCAGCGTGCCTACCACGAGCTCGAGGCGGAAACGGCGCAGCGTATCCGCATGGTAGAGGAA
CTGCGGCAAAAGGAACAGTTGCTGATCCATCAAAGCCGTCTCGCGGCGATGGGGGAGATGCTCGGCTACATCGCGCACCA
GTGGCGCCAGCCGCTTAACGTCCTCGGGCTGCACCTGCAGGTGCTGGGGCTTTCCTACCAGCACGGGACCTTCACCCGGG
AACTCCTGGAGCAGAGCGTGGAAAAAGCGATGGGCATCATCAGGCACCTCTCCAGGACCATCGACGATTTCCGCGACTTT
CTGGTCCTCAACAAGGAGAAGACCCTGTTCCAGGTCGACGAGGTGGTGGTAAAGACGGTCGGGCTCATCGAGGAGAATCT
CAAAAAGGCCGGGATCAACATCGAGGTCGCCTGCACCAACCCCCCGGAGGTAAACGGCTTTCCCAACGAGTACAGCCACG
TGATCCTGAACCTTTTGACCAATGCGAAGGACGCCTTCTCGGAGCGCCAGACTGAGCATCCGGTGATCAGGGTGCATTCG
GGATTCGAGCAGGGGAAGACGGTGGTGACCATCGGCGACAATGCCGGGGGAATCCCCGAAGAGATAATCGGCAAGATCTT
CGACGCCTACTTCACCACCAAGGGGTTGGGAAAAGGAAGCGGGGTTGGCCTTTTTATGTCCAAGATGATCATCGAGAAAA
ACATGGGAGGCAGCCTCACCGTTCGCAACGTCAACGGCGGCGCCGAGTTCAAGATCGAGGTCTGA

Upstream 100 bases:

>100_bases
AAATCCCAATGATAATGCCCCTTCACGCCGGCGTTTCGACCGGCGCACTACAGCGGCAGCAACAAAGGAGCCCGGCCACA
ACCCGCGCAGGAGGCTGCGG

Downstream 100 bases:

>100_bases
ACTCGCCGCAAGCAAGGGAGGTCGACCATGACCCGCGCACCCTCATCTTGGCTCCTGCTGGCGCTGATCTCACTGTTTTG
CTTCAGCGCCGAGGCCGGCG

Product: PAS domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MWVLASFFPLAALALQWLLWERLRPYVWLFFYPAVFLSSWAGGRVAGLLATAFSATVVWYFFIPPEQSFSLEHPSTAIAL
LIFAGMGILFSLFHERLRRADRQIRLTLAEATSGREQLERRMEERTKELVDLVDELRRKETDLRRSQELLKLFIEYAPVP
LAMFDSEMRYLYASRRWRSDYGLGNRPLVKVSHYEIFPEIPQHWKELHQRGLAGEILREEAEEFRRADGTVQWLRWELRP
WYDAGGMVGGIVIFSEDITNRKCAEDALQKLNEELELRVVQRTETLDQILGEREAQNAELQRAYHELEAETAQRIRMVEE
LRQKEQLLIHQSRLAAMGEMLGYIAHQWRQPLNVLGLHLQVLGLSYQHGTFTRELLEQSVEKAMGIIRHLSRTIDDFRDF
LVLNKEKTLFQVDEVVVKTVGLIEENLKKAGINIEVACTNPPEVNGFPNEYSHVILNLLTNAKDAFSERQTEHPVIRVHS
GFEQGKTVVTIGDNAGGIPEEIIGKIFDAYFTTKGLGKGSGVGLFMSKMIIEKNMGGSLTVRNVNGGAEFKIEV

Sequences:

>Translated_554_residues
MWVLASFFPLAALALQWLLWERLRPYVWLFFYPAVFLSSWAGGRVAGLLATAFSATVVWYFFIPPEQSFSLEHPSTAIAL
LIFAGMGILFSLFHERLRRADRQIRLTLAEATSGREQLERRMEERTKELVDLVDELRRKETDLRRSQELLKLFIEYAPVP
LAMFDSEMRYLYASRRWRSDYGLGNRPLVKVSHYEIFPEIPQHWKELHQRGLAGEILREEAEEFRRADGTVQWLRWELRP
WYDAGGMVGGIVIFSEDITNRKCAEDALQKLNEELELRVVQRTETLDQILGEREAQNAELQRAYHELEAETAQRIRMVEE
LRQKEQLLIHQSRLAAMGEMLGYIAHQWRQPLNVLGLHLQVLGLSYQHGTFTRELLEQSVEKAMGIIRHLSRTIDDFRDF
LVLNKEKTLFQVDEVVVKTVGLIEENLKKAGINIEVACTNPPEVNGFPNEYSHVILNLLTNAKDAFSERQTEHPVIRVHS
GFEQGKTVVTIGDNAGGIPEEIIGKIFDAYFTTKGLGKGSGVGLFMSKMIIEKNMGGSLTVRNVNGGAEFKIEV
>Mature_554_residues
MWVLASFFPLAALALQWLLWERLRPYVWLFFYPAVFLSSWAGGRVAGLLATAFSATVVWYFFIPPEQSFSLEHPSTAIAL
LIFAGMGILFSLFHERLRRADRQIRLTLAEATSGREQLERRMEERTKELVDLVDELRRKETDLRRSQELLKLFIEYAPVP
LAMFDSEMRYLYASRRWRSDYGLGNRPLVKVSHYEIFPEIPQHWKELHQRGLAGEILREEAEEFRRADGTVQWLRWELRP
WYDAGGMVGGIVIFSEDITNRKCAEDALQKLNEELELRVVQRTETLDQILGEREAQNAELQRAYHELEAETAQRIRMVEE
LRQKEQLLIHQSRLAAMGEMLGYIAHQWRQPLNVLGLHLQVLGLSYQHGTFTRELLEQSVEKAMGIIRHLSRTIDDFRDF
LVLNKEKTLFQVDEVVVKTVGLIEENLKKAGINIEVACTNPPEVNGFPNEYSHVILNLLTNAKDAFSERQTEHPVIRVHS
GFEQGKTVVTIGDNAGGIPEEIIGKIFDAYFTTKGLGKGSGVGLFMSKMIIEKNMGGSLTVRNVNGGAEFKIEV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=27.8008298755187, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=24.7899159663866, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63632; Mature: 63632

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVLASFFPLAALALQWLLWERLRPYVWLFFYPAVFLSSWAGGRVAGLLATAFSATVVWY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
FFIPPEQSFSLEHPSTAIALLIFAGMGILFSLFHERLRRADRQIRLTLAEATSGREQLER
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCHHHHHH
RMEERTKELVDLVDELRRKETDLRRSQELLKLFIEYAPVPLAMFDSEMRYLYASRRWRSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
YGLGNRPLVKVSHYEIFPEIPQHWKELHQRGLAGEILREEAEEFRRADGTVQWLRWELRP
CCCCCCCEEEEECHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCC
WYDAGGMVGGIVIFSEDITNRKCAEDALQKLNEELELRVVQRTETLDQILGEREAQNAEL
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QRAYHELEAETAQRIRMVEELRQKEQLLIHQSRLAAMGEMLGYIAHQWRQPLNVLGLHLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VLGLSYQHGTFTRELLEQSVEKAMGIIRHLSRTIDDFRDFLVLNKEKTLFQVDEVVVKTV
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLIEENLKKAGINIEVACTNPPEVNGFPNEYSHVILNLLTNAKDAFSERQTEHPVIRVHS
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
GFEQGKTVVTIGDNAGGIPEEIIGKIFDAYFTTKGLGKGSGVGLFMSKMIIEKNMGGSLT
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VRNVNGGAEFKIEV
EEECCCCCEEEEEC
>Mature Secondary Structure
MWVLASFFPLAALALQWLLWERLRPYVWLFFYPAVFLSSWAGGRVAGLLATAFSATVVWY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEE
FFIPPEQSFSLEHPSTAIALLIFAGMGILFSLFHERLRRADRQIRLTLAEATSGREQLER
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEHHHCCCHHHHHH
RMEERTKELVDLVDELRRKETDLRRSQELLKLFIEYAPVPLAMFDSEMRYLYASRRWRSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
YGLGNRPLVKVSHYEIFPEIPQHWKELHQRGLAGEILREEAEEFRRADGTVQWLRWELRP
CCCCCCCEEEEECHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCC
WYDAGGMVGGIVIFSEDITNRKCAEDALQKLNEELELRVVQRTETLDQILGEREAQNAEL
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QRAYHELEAETAQRIRMVEELRQKEQLLIHQSRLAAMGEMLGYIAHQWRQPLNVLGLHLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VLGLSYQHGTFTRELLEQSVEKAMGIIRHLSRTIDDFRDFLVLNKEKTLFQVDEVVVKTV
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLIEENLKKAGINIEVACTNPPEVNGFPNEYSHVILNLLTNAKDAFSERQTEHPVIRVHS
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
GFEQGKTVVTIGDNAGGIPEEIIGKIFDAYFTTKGLGKGSGVGLFMSKMIIEKNMGGSLT
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VRNVNGGAEFKIEV
EEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]