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The map label for this gene is mraW

Identifier: 197116881

GI number: 197116881

Start: 582897

End: 583835

Strand: Direct

Name: mraW

Synonym: Gbem_0483

Alternate gene names: 197116881

Gene position: 582897-583835 (Clockwise)

Preceding gene: 308535167

Following gene: 197116882

Centisome position: 12.63

GC content: 64.22

Gene sequence:

>939_bases
ATGGAAGACTTCCACCACATATCGGTACTGCCGGACGAGGTGCTGCAGGCGCTCTCCCCGAGATCGGGGGGGGTATACGT
GGACGGCACCTTGGGAGGCGCCGGGCACGCGGGGCTCATACTCACCGCCAGCGCCCCGGACGGCCAGCTGATCGGATTCG
ACCGGGACGAGGAAGCCATAGCCGTGGCACGTGAGCGGCTCCAGGTTTTCGGCGGCCGGGTCCGGATCATCCACCGCAAT
TTTGCCGGCATCGCTCAGGCTCTGGCGGAGATAGGTGTGGACGGGATCGACGGCTTCGTACTGGACCTTGGGGTGTCGAG
CCACCAGCTGGACCGCGACGAGCGGGGCTTCAGCTTCATGCACGACGCGCCGCTCGACATGCGCATGGACAGAAGCTCCG
GCCAGAGCGCGGCGGACCTGGTGAACACCCTCCCCGAGGGTGAGCTTTACCGGATCATCTCCGAGTACGGCGAGGAGCGC
TGGGCCAAGCGGGTCGCCTCTTTCATAGTGAAGGCCAGGGACGAGCGTCCCATCGAGACCACGCTTCAGTTGGTGGACGT
GATCAAGGGGTCGATACCGAAGGCAAAGTGGGAGGAGCGGCTTCATCCTGCCACCCGGACCTTCCAGGCCTTGAGGATCG
CGGTAAACGAGGAGTTGAAGAGCCTAGAAGAAGGGCTTGAGGGGCTGCTCTCCCTGCTGAAGCAGGGGGGACGCGGCGCG
GTGATCTCCTTTCACTCGCTGGAAGACCGTATCGTGAAGGAGGGCTTTCGCGCGGCGGCCACCGGTTGCACCTGCCCCAA
GGAACTCCCCATCTGCATCTGCGGACGGGTTCCGAGGTTCAAGCTGGTGACCAGGAAGCCGATAACCGCAGGCGAGGCCG
AAGTGGCAGCTAATCCGCGCTCCAGGAGCGCGAAGCTGCGGGTAGTGGAGAAGATTTAG

Upstream 100 bases:

>100_bases
GGTCGACATCTACAGCCAGTCGGTCTGGGCCAGGGTCTGCGAGCAGGATGAGCAAAACTTCCCGGTGGATTCCGCGGCGC
TTGCGGGACTCGGCCTCTAG

Downstream 100 bases:

>100_bases
AGAGGTAGCATCATGTCGCAAAGCAAGGTTGCCTACGGCAAGGTCGCCGCACCGGCAAGACCGGGCGCGGTCCCCAGGGA
GAGCTGGATCAGCTTTCCCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MEDFHHISVLPDEVLQALSPRSGGVYVDGTLGGAGHAGLILTASAPDGQLIGFDRDEEAIAVARERLQVFGGRVRIIHRN
FAGIAQALAEIGVDGIDGFVLDLGVSSHQLDRDERGFSFMHDAPLDMRMDRSSGQSAADLVNTLPEGELYRIISEYGEER
WAKRVASFIVKARDERPIETTLQLVDVIKGSIPKAKWEERLHPATRTFQALRIAVNEELKSLEEGLEGLLSLLKQGGRGA
VISFHSLEDRIVKEGFRAAATGCTCPKELPICICGRVPRFKLVTRKPITAGEAEVAANPRSRSAKLRVVEKI

Sequences:

>Translated_312_residues
MEDFHHISVLPDEVLQALSPRSGGVYVDGTLGGAGHAGLILTASAPDGQLIGFDRDEEAIAVARERLQVFGGRVRIIHRN
FAGIAQALAEIGVDGIDGFVLDLGVSSHQLDRDERGFSFMHDAPLDMRMDRSSGQSAADLVNTLPEGELYRIISEYGEER
WAKRVASFIVKARDERPIETTLQLVDVIKGSIPKAKWEERLHPATRTFQALRIAVNEELKSLEEGLEGLLSLLKQGGRGA
VISFHSLEDRIVKEGFRAAATGCTCPKELPICICGRVPRFKLVTRKPITAGEAEVAANPRSRSAKLRVVEKI
>Mature_312_residues
MEDFHHISVLPDEVLQALSPRSGGVYVDGTLGGAGHAGLILTASAPDGQLIGFDRDEEAIAVARERLQVFGGRVRIIHRN
FAGIAQALAEIGVDGIDGFVLDLGVSSHQLDRDERGFSFMHDAPLDMRMDRSSGQSAADLVNTLPEGELYRIISEYGEER
WAKRVASFIVKARDERPIETTLQLVDVIKGSIPKAKWEERLHPATRTFQALRIAVNEELKSLEEGLEGLLSLLKQGGRGA
VISFHSLEDRIVKEGFRAAATGCTCPKELPICICGRVPRFKLVTRKPITAGEAEVAANPRSRSAKLRVVEKI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=37.5366568914956, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI165377202, Length=208, Percent_Identity=39.4230769230769, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=46.0815047021944, Blast_Score=257, Evalue=6e-70,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=38.2978723404255, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=38.2978723404255, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_GEOBB (B5EBP3)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137308.1
- ProteinModelPortal:   B5EBP3
- GeneID:   6783312
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0483
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34109; Mature: 34109

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDFHHISVLPDEVLQALSPRSGGVYVDGTLGGAGHAGLILTASAPDGQLIGFDRDEEAI
CCCCHHEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCEECCCCCHHHH
AVARERLQVFGGRVRIIHRNFAGIAQALAEIGVDGIDGFVLDLGVSSHQLDRDERGFSFM
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCCCCCEE
HDAPLDMRMDRSSGQSAADLVNTLPEGELYRIISEYGEERWAKRVASFIVKARDERPIET
CCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TLQLVDVIKGSIPKAKWEERLHPATRTFQALRIAVNEELKSLEEGLEGLLSLLKQGGRGA
HHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VISFHSLEDRIVKEGFRAAATGCTCPKELPICICGRVPRFKLVTRKPITAGEAEVAANPR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCHHCCCCC
SRSAKLRVVEKI
CCCCEEEEEECC
>Mature Secondary Structure
MEDFHHISVLPDEVLQALSPRSGGVYVDGTLGGAGHAGLILTASAPDGQLIGFDRDEEAI
CCCCHHEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCEECCCCCHHHH
AVARERLQVFGGRVRIIHRNFAGIAQALAEIGVDGIDGFVLDLGVSSHQLDRDERGFSFM
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCCCEEEECCCCHHHCCCCCCCCCEE
HDAPLDMRMDRSSGQSAADLVNTLPEGELYRIISEYGEERWAKRVASFIVKARDERPIET
CCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TLQLVDVIKGSIPKAKWEERLHPATRTFQALRIAVNEELKSLEEGLEGLLSLLKQGGRGA
HHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VISFHSLEDRIVKEGFRAAATGCTCPKELPICICGRVPRFKLVTRKPITAGEAEVAANPR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCHHCCCCC
SRSAKLRVVEKI
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA