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The map label for this gene is mltD [H]

Identifier: 197116870

GI number: 197116870

Start: 570523

End: 571977

Strand: Direct

Name: mltD [H]

Synonym: Gbem_0472

Alternate gene names: 197116870

Gene position: 570523-571977 (Clockwise)

Preceding gene: 197116869

Following gene: 197116871

Centisome position: 12.36

GC content: 62.68

Gene sequence:

>1455_bases
ATGATCAAAACGACCCTTATCATACTGTCACTGTTGTTCTCTACCACCGCTTTCGCCGCGCCGGCCAGCACGGCCGCCGA
ACAGCGAGACCTGGCCCGGGCCATGCAGGTCGCCTCCAACTCCTCCGACGTCGCGCTCTCCAAAGGGCTCGGCCTCACCC
CCTCCGATTTTTCCGACGACGAAGATTTCAGCGACGATTCCCAGGATTACGACCTTAAGCTGCCGGAGATGGAACTTCCC
GACTCCGATATTCCGCTTACCTTCAACACCAAGGTGGACTACTTCGTCCGCTACTTCCAGGGGCCGGCGCGCCCGGTCTT
CGCCAAGTGGCTCTCCCGCAGCGAGCGCTATATCCCGATGATGAAGACCGTGCTCAAGAAGGAAGGGCTCCCGGAGGATC
TGGTCTACCTGGCCATGATCGAGAGCGGATTCATGACCCACGCGGTGTCGGTGGCGAGCGCGGTCGGCCCGTGGCAGTTC
ATGCCGGCAACGGGAAAGCGCTATGCGCTGAGGATCGACCCCTGGGTCGACGAGAGACGCGATCCCCTGAAGGCCACGAT
CGCCGCGGCGCTCTATTTAAAAGAGCTCTATGCCCTGTTCAACAACGACTGGTACCTGGCGGCTGCAGGGTACAACGCGG
GTGAGAACAAGATCCTGCGCGCCATCAACATGTACAACACCCGCGACTTCTGGGAGATCTCCAAGGGGAGCTACCTGGCC
CGCGAGACCAAGGATTACGTCCCGAAACTTCTGGCTGCAGCCATCATCGCCAAGGAACCGGCCAAGCACGGCTTCGCAGA
CGTGGCCTACCTCCCCCCCATCGAATTCGACGTGGTGAAGATCCCGTCGCGCACGGACCTGGAGGTGGTAGCCAAGGCTT
GCGGGGTCTCGGTGCAGACCATCCGCGACTTGAACCCTGCGCTGCGCCGCGGCTCCACTCCGCCGGACTACCCGGGGTAC
CAGCTGAAGGTTCCCCGAGGGGCGGCAGCCTCCTTCGAGACCGAGTACGCCAAGGTGCCCGAGTCCGAGCGCTACACCGA
GAAGGTGAAGCACGTGCGCTACCGCGCCAAGAAGAAGGACACCCTCGTCTCCATCGCCAGACGCTTCAAGACCACGCCGC
AGGCTATTGCCGAGCTGAACGACCTGGGGCACAAGAAGGCCGCGAAGCTCCACGGCAAGGTGCTGACCGTCCCGGTGCTG
GTAGCGGCTCAGGGCGAGCAGACATCGACTCCGGCCCTGGCTGAGGCGAAGGCGGAGGCCAAGGAGTTCCACAAGTACTA
CACCGTGAAGAAGGGCGATACCCTTGCCTCCCTGGCCAAAAGGTTCAACGTCACGGCGCGGGTTCTCTCGGCCTGGAACA
ACCTGAAGACCAGGGTGGCGCTGAAACCCGGCAAGCGCATCATCGTCGCCAAGTACCAGGAGAAGAAGGGCGCCGGGGCA
CGGGACTCGGGATAG

Upstream 100 bases:

>100_bases
ACCCTGCTAAAAGCCCCTTTGAATTGACAACTCCGCTAAATTCCGCTATTCTGCGCGCCGGTGCGTTCGCCAGCGGTACA
ACCCCGTAAACAGGTATTGA

Downstream 100 bases:

>100_bases
CCGGCAGCGCACCAAACACCCACACCTAACAATTCACCCCTCAAGCAGCACGAAAGGATAAGCCAGACCATGTATAACCT
TCTCATCTCCGCAGGCGCGG

Product: lytic transglycosylase, SLT, LysM/LysM domain-containing protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIKTTLIILSLLFSTTAFAAPASTAAEQRDLARAMQVASNSSDVALSKGLGLTPSDFSDDEDFSDDSQDYDLKLPEMELP
DSDIPLTFNTKVDYFVRYFQGPARPVFAKWLSRSERYIPMMKTVLKKEGLPEDLVYLAMIESGFMTHAVSVASAVGPWQF
MPATGKRYALRIDPWVDERRDPLKATIAAALYLKELYALFNNDWYLAAAGYNAGENKILRAINMYNTRDFWEISKGSYLA
RETKDYVPKLLAAAIIAKEPAKHGFADVAYLPPIEFDVVKIPSRTDLEVVAKACGVSVQTIRDLNPALRRGSTPPDYPGY
QLKVPRGAAASFETEYAKVPESERYTEKVKHVRYRAKKKDTLVSIARRFKTTPQAIAELNDLGHKKAAKLHGKVLTVPVL
VAAQGEQTSTPALAEAKAEAKEFHKYYTVKKGDTLASLAKRFNVTARVLSAWNNLKTRVALKPGKRIIVAKYQEKKGAGA
RDSG

Sequences:

>Translated_484_residues
MIKTTLIILSLLFSTTAFAAPASTAAEQRDLARAMQVASNSSDVALSKGLGLTPSDFSDDEDFSDDSQDYDLKLPEMELP
DSDIPLTFNTKVDYFVRYFQGPARPVFAKWLSRSERYIPMMKTVLKKEGLPEDLVYLAMIESGFMTHAVSVASAVGPWQF
MPATGKRYALRIDPWVDERRDPLKATIAAALYLKELYALFNNDWYLAAAGYNAGENKILRAINMYNTRDFWEISKGSYLA
RETKDYVPKLLAAAIIAKEPAKHGFADVAYLPPIEFDVVKIPSRTDLEVVAKACGVSVQTIRDLNPALRRGSTPPDYPGY
QLKVPRGAAASFETEYAKVPESERYTEKVKHVRYRAKKKDTLVSIARRFKTTPQAIAELNDLGHKKAAKLHGKVLTVPVL
VAAQGEQTSTPALAEAKAEAKEFHKYYTVKKGDTLASLAKRFNVTARVLSAWNNLKTRVALKPGKRIIVAKYQEKKGAGA
RDSG
>Mature_484_residues
MIKTTLIILSLLFSTTAFAAPASTAAEQRDLARAMQVASNSSDVALSKGLGLTPSDFSDDEDFSDDSQDYDLKLPEMELP
DSDIPLTFNTKVDYFVRYFQGPARPVFAKWLSRSERYIPMMKTVLKKEGLPEDLVYLAMIESGFMTHAVSVASAVGPWQF
MPATGKRYALRIDPWVDERRDPLKATIAAALYLKELYALFNNDWYLAAAGYNAGENKILRAINMYNTRDFWEISKGSYLA
RETKDYVPKLLAAAIIAKEPAKHGFADVAYLPPIEFDVVKIPSRTDLEVVAKACGVSVQTIRDLNPALRRGSTPPDYPGY
QLKVPRGAAASFETEYAKVPESERYTEKVKHVRYRAKKKDTLVSIARRFKTTPQAIAELNDLGHKKAAKLHGKVLTVPVL
VAAQGEQTSTPALAEAKAEAKEFHKYYTVKKGDTLASLAKRFNVTARVLSAWNNLKTRVALKPGKRIIVAKYQEKKGAGA
RDSG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=376, Percent_Identity=29.7872340425532, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 53747; Mature: 53747

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTTLIILSLLFSTTAFAAPASTAAEQRDLARAMQVASNSSDVALSKGLGLTPSDFSDD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
EDFSDDSQDYDLKLPEMELPDSDIPLTFNTKVDYFVRYFQGPARPVFAKWLSRSERYIPM
CCCCCCCCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
MKTVLKKEGLPEDLVYLAMIESGFMTHAVSVASAVGPWQFMPATGKRYALRIDPWVDERR
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCCCCC
DPLKATIAAALYLKELYALFNNDWYLAAAGYNAGENKILRAINMYNTRDFWEISKGSYLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCHH
RETKDYVPKLLAAAIIAKEPAKHGFADVAYLPPIEFDVVKIPSRTDLEVVAKACGVSVQT
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHCCHHHH
IRDLNPALRRGSTPPDYPGYQLKVPRGAAASFETEYAKVPESERYTEKVKHVRYRAKKKD
HHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
TLVSIARRFKTTPQAIAELNDLGHKKAAKLHGKVLTVPVLVAAQGEQTSTPALAEAKAEA
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHH
KEFHKYYTVKKGDTLASLAKRFNVTARVLSAWNNLKTRVALKPGKRIIVAKYQEKKGAGA
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCC
RDSG
CCCC
>Mature Secondary Structure
MIKTTLIILSLLFSTTAFAAPASTAAEQRDLARAMQVASNSSDVALSKGLGLTPSDFSDD
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
EDFSDDSQDYDLKLPEMELPDSDIPLTFNTKVDYFVRYFQGPARPVFAKWLSRSERYIPM
CCCCCCCCCCCEECCCCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
MKTVLKKEGLPEDLVYLAMIESGFMTHAVSVASAVGPWQFMPATGKRYALRIDPWVDERR
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCCCCC
DPLKATIAAALYLKELYALFNNDWYLAAAGYNAGENKILRAINMYNTRDFWEISKGSYLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEECCCCCHH
RETKDYVPKLLAAAIIAKEPAKHGFADVAYLPPIEFDVVKIPSRTDLEVVAKACGVSVQT
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHCCHHHH
IRDLNPALRRGSTPPDYPGYQLKVPRGAAASFETEYAKVPESERYTEKVKHVRYRAKKKD
HHHCCHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
TLVSIARRFKTTPQAIAELNDLGHKKAAKLHGKVLTVPVLVAAQGEQTSTPALAEAKAEA
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHH
KEFHKYYTVKKGDTLASLAKRFNVTARVLSAWNNLKTRVALKPGKRIIVAKYQEKKGAGA
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCC
RDSG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]