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The map label for this gene is merA [H]

Identifier: 197116855

GI number: 197116855

Start: 549832

End: 551355

Strand: Direct

Name: merA [H]

Synonym: Gbem_0457

Alternate gene names: 197116855

Gene position: 549832-551355 (Clockwise)

Preceding gene: 308535164

Following gene: 197116856

Centisome position: 11.91

GC content: 66.08

Gene sequence:

>1524_bases
ATGAGCGACCAATTTGTGTTTCAGCCGGACACGGCGGAGAACAGGGAACTTGAGGCCAACGTGTGCCCGAAAAGTTGGAT
CAACCCGGTGCCATCGGGACGTTACAACCTGGTGGTGGTCGGCGCCGGGACCGCGGGCCTTGTCTGCGCCGCTGGGGCGG
CTTCGTTGGGGGCGCGCGTGGCTCTGGTCGAGCGGCTGGCTCTCGGGGGAGACTGCCTGAACGTCGGCTGCGTCCCGTCG
AAAGCGCTGATCCGTGCCTCGCGTGCCGTCTTCGATGCCCGTCATAGCGGCGGCTTCGGCGTAGTCGGGGGCGACGCGCT
GCTGGCCGATTTTGCCGCGGCGCTGCAGCGGATGCGCAGGTTGAGGGCCGGGATCAGCCGCCACGACTCGGCGCTCCGCT
TCCGGGATGAGTTGGGTGTCGACGTCTACCTGGGACAAGGGACCTTCACCGCCCCGGACACCCTCCAAGTCGAAGGGGCC
GCGCTCCATTTCGCCAAGGCTGCGCTCTGCACCGGCGCCCGGGCGGCCATCCCCCCGGTTCCGGGTCTGGAGGAGGCGGG
ATGCCTGACCAACGAGACGGTCTTCTCGCTCACCTCGCTTCCCGCCAGGCTTGCGGTGATTGGTTCGGGCCCCGTCGGTT
GCGAGCTGGCGCAGGCCTTCGCCCGTTTCGGAAGCAAGGTGACCCTGGTCGAGCGGGGATCTGGGATTCTGGGGCGCGAG
GACCGCGATGCGGCCGCTATCCTCGAAACGGCTTTCCGGCGTGAGGGGATCGAACTGGAACTCGGGGCGAAGCTCGTCAG
GGCCTGGAGCAGCGGCTCGGAAAAACGGCTTCTCCTGGAGCGGGACGGGGTAGGGTTCGAGATTGCCGTCGACGCCATTC
TGGTCGGAGCGGGGCGCGCGCCCAACACCGAGGGGCTGGGGCTCGAGGCCGCGGGAGTGGAATACGACAAGAGCGGGGTC
AAGGTGAACGATTACCTGCAGACCACCAACCGGCGCATCTATGCCGCCGGAGACATCTGCTCCGGCTACAAGTTCACCCA
CGTAGCCGACGCGCAGGCGAGGATAGTGATAGAAAACTCTCTTTTTCCGGGGAGGAGGAAGAACTCCAGCCTGACCGTGC
CGTGGTGCACCTATACCGACCCCGAGGTGGCGCATGTCGGGATGTACCAAGCGGACGCCCTGGCGCGCGGGTTGAAGGTC
GAGACCCTCACCATCCCCTTTGGCGATGTGGATCGTGCCGTCCTGGACGGGGAGAGCGAAGGGTTTGCGCGGGTGCACCT
GAAAAAAGGGAGCGACGTCATCCTGGGCGCCACCATAGTCGCGCGCCACGCGGGGGAGATGATCGGCGAGGTCGCCCTTG
CCATAAACGCCGGCTTGGGGCTCTCCGCCATAGGGCGCACCATCCATCCCTACCCCACTCAGGCGGAGAGCCTCAGAAAG
CTTGCCGACAGCTACAACCGCGGCAGGTTGACCCCGCGAGTCAAGAAGCTGATGGGGGCATGGCTTCGCTGGCAAAGGGA
ATAA

Upstream 100 bases:

>100_bases
TGAATTATCCTCGCCTAAGTTGCTGCTTTGCCCATGCGAGCGGCTACTGTATCTTCACTATGTTAATGCATTGAGGAAAA
CCTTGCAAAGGATGACTGCG

Downstream 100 bases:

>100_bases
AAACCAGATAAAGATAAAGTGAGATTAAGATTAAGATTGAGATTGAGATTGAGATTGAGAAAAATCACTTCTCAGTGTAC
GTAGATCATCTACCGATAAT

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSDQFVFQPDTAENRELEANVCPKSWINPVPSGRYNLVVVGAGTAGLVCAAGAASLGARVALVERLALGGDCLNVGCVPS
KALIRASRAVFDARHSGGFGVVGGDALLADFAAALQRMRRLRAGISRHDSALRFRDELGVDVYLGQGTFTAPDTLQVEGA
ALHFAKAALCTGARAAIPPVPGLEEAGCLTNETVFSLTSLPARLAVIGSGPVGCELAQAFARFGSKVTLVERGSGILGRE
DRDAAAILETAFRREGIELELGAKLVRAWSSGSEKRLLLERDGVGFEIAVDAILVGAGRAPNTEGLGLEAAGVEYDKSGV
KVNDYLQTTNRRIYAAGDICSGYKFTHVADAQARIVIENSLFPGRRKNSSLTVPWCTYTDPEVAHVGMYQADALARGLKV
ETLTIPFGDVDRAVLDGESEGFARVHLKKGSDVILGATIVARHAGEMIGEVALAINAGLGLSAIGRTIHPYPTQAESLRK
LADSYNRGRLTPRVKKLMGAWLRWQRE

Sequences:

>Translated_507_residues
MSDQFVFQPDTAENRELEANVCPKSWINPVPSGRYNLVVVGAGTAGLVCAAGAASLGARVALVERLALGGDCLNVGCVPS
KALIRASRAVFDARHSGGFGVVGGDALLADFAAALQRMRRLRAGISRHDSALRFRDELGVDVYLGQGTFTAPDTLQVEGA
ALHFAKAALCTGARAAIPPVPGLEEAGCLTNETVFSLTSLPARLAVIGSGPVGCELAQAFARFGSKVTLVERGSGILGRE
DRDAAAILETAFRREGIELELGAKLVRAWSSGSEKRLLLERDGVGFEIAVDAILVGAGRAPNTEGLGLEAAGVEYDKSGV
KVNDYLQTTNRRIYAAGDICSGYKFTHVADAQARIVIENSLFPGRRKNSSLTVPWCTYTDPEVAHVGMYQADALARGLKV
ETLTIPFGDVDRAVLDGESEGFARVHLKKGSDVILGATIVARHAGEMIGEVALAINAGLGLSAIGRTIHPYPTQAESLRK
LADSYNRGRLTPRVKKLMGAWLRWQRE
>Mature_506_residues
SDQFVFQPDTAENRELEANVCPKSWINPVPSGRYNLVVVGAGTAGLVCAAGAASLGARVALVERLALGGDCLNVGCVPSK
ALIRASRAVFDARHSGGFGVVGGDALLADFAAALQRMRRLRAGISRHDSALRFRDELGVDVYLGQGTFTAPDTLQVEGAA
LHFAKAALCTGARAAIPPVPGLEEAGCLTNETVFSLTSLPARLAVIGSGPVGCELAQAFARFGSKVTLVERGSGILGRED
RDAAAILETAFRREGIELELGAKLVRAWSSGSEKRLLLERDGVGFEIAVDAILVGAGRAPNTEGLGLEAAGVEYDKSGVK
VNDYLQTTNRRIYAAGDICSGYKFTHVADAQARIVIENSLFPGRRKNSSLTVPWCTYTDPEVAHVGMYQADALARGLKVE
TLTIPFGDVDRAVLDGESEGFARVHLKKGSDVILGATIVARHAGEMIGEVALAINAGLGLSAIGRTIHPYPTQAESLRKL
ADSYNRGRLTPRVKKLMGAWLRWQRE

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=29.8728813559322, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=27.9317697228145, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI291045266, Length=475, Percent_Identity=25.6842105263158, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI22035672, Length=490, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI33519430, Length=461, Percent_Identity=24.0780911062907, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI33519428, Length=461, Percent_Identity=24.0780911062907, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI33519426, Length=461, Percent_Identity=24.0780911062907, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI148277065, Length=461, Percent_Identity=24.0780911062907, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI148277071, Length=461, Percent_Identity=24.0780911062907, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=31.6593886462882, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=30.1255230125523, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=29.3302540415704, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=25.2676659528908, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789065, Length=183, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=32.1120689655172, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17557007, Length=495, Percent_Identity=25.6565656565657, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=26.036866359447, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=26.036866359447, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=501, Percent_Identity=25.1497005988024, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=30.5732484076433, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=26.9396551724138, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325240, Length=488, Percent_Identity=25.2049180327869, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=33.1168831168831, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI17737741, Length=500, Percent_Identity=28.2, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=25.8726899383984, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=25.8726899383984, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640551, Length=484, Percent_Identity=25.8264462809917, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 53748; Mature: 53617

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQFVFQPDTAENRELEANVCPKSWINPVPSGRYNLVVVGAGTAGLVCAAGAASLGARV
CCCCEEECCCCCCCCEEECCCCCHHHCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHH
ALVERLALGGDCLNVGCVPSKALIRASRAVFDARHSGGFGVVGGDALLADFAAALQRMRR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
LRAGISRHDSALRFRDELGVDVYLGQGTFTAPDTLQVEGAALHFAKAALCTGARAAIPPV
HHHHHHHCCHHHHHHHHCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCC
PGLEEAGCLTNETVFSLTSLPARLAVIGSGPVGCELAQAFARFGSKVTLVERGSGILGRE
CCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCC
DRDAAAILETAFRREGIELELGAKLVRAWSSGSEKRLLLERDGVGFEIAVDAILVGAGRA
CCHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEECCCC
PNTEGLGLEAAGVEYDKSGVKVNDYLQTTNRRIYAAGDICSGYKFTHVADAQARIVIENS
CCCCCCCEEECCCEECCCCCEEHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEECC
LFPGRRKNSSLTVPWCTYTDPEVAHVGMYQADALARGLKVETLTIPFGDVDRAVLDGESE
CCCCCCCCCEEEEEEEECCCCCHHEECCHHHHHHHCCCEEEEEEECCCCCCHHHCCCCCC
GFARVHLKKGSDVILGATIVARHAGEMIGEVALAINAGLGLSAIGRTIHPYPTQAESLRK
CEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCCCCHHHHHH
LADSYNRGRLTPRVKKLMGAWLRWQRE
HHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDQFVFQPDTAENRELEANVCPKSWINPVPSGRYNLVVVGAGTAGLVCAAGAASLGARV
CCCEEECCCCCCCCEEECCCCCHHHCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHH
ALVERLALGGDCLNVGCVPSKALIRASRAVFDARHSGGFGVVGGDALLADFAAALQRMRR
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
LRAGISRHDSALRFRDELGVDVYLGQGTFTAPDTLQVEGAALHFAKAALCTGARAAIPPV
HHHHHHHCCHHHHHHHHCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCC
PGLEEAGCLTNETVFSLTSLPARLAVIGSGPVGCELAQAFARFGSKVTLVERGSGILGRE
CCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCC
DRDAAAILETAFRREGIELELGAKLVRAWSSGSEKRLLLERDGVGFEIAVDAILVGAGRA
CCHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEECCCC
PNTEGLGLEAAGVEYDKSGVKVNDYLQTTNRRIYAAGDICSGYKFTHVADAQARIVIENS
CCCCCCCEEECCCEECCCCCEEHHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEECC
LFPGRRKNSSLTVPWCTYTDPEVAHVGMYQADALARGLKVETLTIPFGDVDRAVLDGESE
CCCCCCCCCEEEEEEEECCCCCHHEECCHHHHHHHCCCEEEEEEECCCCCCHHHCCCCCC
GFARVHLKKGSDVILGATIVARHAGEMIGEVALAINAGLGLSAIGRTIHPYPTQAESLRK
CEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCCCCHHHHHH
LADSYNRGRLTPRVKKLMGAWLRWQRE
HHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]