Click here to switch to the map view.

The map label for this gene is dapF [H]

Identifier: 197116758

GI number: 197116758

Start: 437484

End: 438320

Strand: Reverse

Name: dapF [H]

Synonym: Gbem_0358

Alternate gene names: 197116758

Gene position: 438320-437484 (Counterclockwise)

Preceding gene: 197116762

Following gene: 197116757

Centisome position: 9.5

GC content: 60.22

Gene sequence:

>837_bases
ATGAAATTCACAAAGATGCAGGGTGCAGGCAACGACTACGTGTATGTCAACTGTTTCGAAGAGGCGGTGCATGACCCTGC
CGCGGTGGCGATAAAGGTCTCCAACCGCAACTTCGGCATCGGATCGGACGGGCTGATCCTGATCATGCCGAGCGAAGTGG
CCGACGTAAGGATGCGGATGTTCAACTCGGATGGATCAGAAAGCGAGATGTGCGGCAACGGCATCCGCTGCGTGGCGAAA
TATGCCTACGACCACGGAATCGTGACGAAAAATGAGATAACTGCGGAAACCGGCGCCGGGATCCTGACCCTGCAGCTTTT
CACCGACGCCGGTTGCAAGGTGGAAAAGGTGCGGGTGAACATGGGGCCGCCGAGGCTGACCAGGAAGGAAATCCCCATGC
TGGGGGAACCGGAGGAGCGGGTGGTCAACGTGCCGCTCAACGTGCTCCACTCCAGCTTCAACATCACCTGCGCCTCCATG
GGGAACCCCCACTGCGTCATCTTCGTGGATGACGTGGACACGTTCCAGGTCGAGAAGTACGGTCCGCTGATCGAGAATCA
CGAGCTGTTCCCGCGCCGCACCAACGTGGAATTCGTTCAGATCGTCTCCCGCACCGAGGTGCGCCAGCGCACCTGGGAGC
GCGGCGCCGGCGAAACGCTTGCCTGCGGCACCGGTTCCAGCGCAGTCACCGCCGCCTGCGTCCTGAACGGGCTGACAGAG
AAGCGCATCTTGAACCACTTGACCGGCGGCGATCTCGAGATGGAGTGGGGGGAGGACGGCAACATCTACATGACCGGCCC
GGCGGTTGAAGTGTTTACCGGAGAAATCGACTTATGA

Upstream 100 bases:

>100_bases
TTAAAAGATGTCCCCGCGGTCAAGCTACTCCGGTTTTTTCTTGTTGGACCCAGCCGATTTTGCTATTAGAGAGTGATACT
TTTCCCGGGGGTCTTTCCTT

Downstream 100 bases:

>100_bases
ACTGCCCAATCTGCAGCAAATGGCAGGACGATCCGGACCAGAGGGTGGTCGAACTGAAGCACACCCTGGTCTCCTTGAAC
CGGGACCAGTTCTTTGCCGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKFTKMQGAGNDYVYVNCFEEAVHDPAAVAIKVSNRNFGIGSDGLILIMPSEVADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKNEITAETGAGILTLQLFTDAGCKVEKVRVNMGPPRLTRKEIPMLGEPEERVVNVPLNVLHSSFNITCASM
GNPHCVIFVDDVDTFQVEKYGPLIENHELFPRRTNVEFVQIVSRTEVRQRTWERGAGETLACGTGSSAVTAACVLNGLTE
KRILNHLTGGDLEMEWGEDGNIYMTGPAVEVFTGEIDL

Sequences:

>Translated_278_residues
MKFTKMQGAGNDYVYVNCFEEAVHDPAAVAIKVSNRNFGIGSDGLILIMPSEVADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKNEITAETGAGILTLQLFTDAGCKVEKVRVNMGPPRLTRKEIPMLGEPEERVVNVPLNVLHSSFNITCASM
GNPHCVIFVDDVDTFQVEKYGPLIENHELFPRRTNVEFVQIVSRTEVRQRTWERGAGETLACGTGSSAVTAACVLNGLTE
KRILNHLTGGDLEMEWGEDGNIYMTGPAVEVFTGEIDL
>Mature_278_residues
MKFTKMQGAGNDYVYVNCFEEAVHDPAAVAIKVSNRNFGIGSDGLILIMPSEVADVRMRMFNSDGSESEMCGNGIRCVAK
YAYDHGIVTKNEITAETGAGILTLQLFTDAGCKVEKVRVNMGPPRLTRKEIPMLGEPEERVVNVPLNVLHSSFNITCASM
GNPHCVIFVDDVDTFQVEKYGPLIENHELFPRRTNVEFVQIVSRTEVRQRTWERGAGETLACGTGSSAVTAACVLNGLTE
KRILNHLTGGDLEMEWGEDGNIYMTGPAVEVFTGEIDL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=39.5017793594306, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30486; Mature: 30486

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKMQGAGNDYVYVNCFEEAVHDPAAVAIKVSNRNFGIGSDGLILIMPSEVADVRMRM
CCCEECCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHH
FNSDGSESEMCGNGIRCVAKYAYDHGIVTKNEITAETGAGILTLQLFTDAGCKVEKVRVN
HCCCCCCHHHCCCCCEEEEEHHHCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEEE
MGPPRLTRKEIPMLGEPEERVVNVPLNVLHSSFNITCASMGNPHCVIFVDDVDTFQVEKY
CCCCCCCHHCCCCCCCCHHHEEECCHHHEECCCCEEEEECCCCEEEEEEECCCEEEECCC
GPLIENHELFPRRTNVEFVQIVSRTEVRQRTWERGAGETLACGTGSSAVTAACVLNGLTE
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHH
KRILNHLTGGDLEMEWGEDGNIYMTGPAVEVFTGEIDL
HHHHHHCCCCCEEEEECCCCCEEEECCEEEEEEEEECC
>Mature Secondary Structure
MKFTKMQGAGNDYVYVNCFEEAVHDPAAVAIKVSNRNFGIGSDGLILIMPSEVADVRMRM
CCCEECCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHH
FNSDGSESEMCGNGIRCVAKYAYDHGIVTKNEITAETGAGILTLQLFTDAGCKVEKVRVN
HCCCCCCHHHCCCCCEEEEEHHHCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEEE
MGPPRLTRKEIPMLGEPEERVVNVPLNVLHSSFNITCASMGNPHCVIFVDDVDTFQVEKY
CCCCCCCHHCCCCCCCCHHHEEECCHHHEECCCCEEEEECCCCEEEEEEECCCEEEECCC
GPLIENHELFPRRTNVEFVQIVSRTEVRQRTWERGAGETLACGTGSSAVTAACVLNGLTE
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCHH
KRILNHLTGGDLEMEWGEDGNIYMTGPAVEVFTGEIDL
HHHHHHCCCCCEEEEECCCCCEEEECCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA