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The map label for this gene is nasA [H]

Identifier: 197104437

GI number: 197104437

Start: 1069135

End: 1071777

Strand: Direct

Name: nasA [H]

Synonym: PHZ_c0971

Alternate gene names: 197104437

Gene position: 1069135-1071777 (Clockwise)

Preceding gene: 197104436

Following gene: 197104438

Centisome position: 26.75

GC content: 76.01

Gene sequence:

>2643_bases
GTGAGGACGACCTGCCCCTACTGCGGCGTCGGGTGCGGGATCTCGGCTGAGGCCGAGGGCCGAGCGCTGTCGGTGGCGGG
CGATCCGGCGCATCCGGCCAACCTCGGGCGGCTGTGCTCGAAGGGGAGCGCCCTGGGGGCCACGGTCGGCCTGGAGGGGC
GGCTGCTGCATCCGACGATCGGCGATCGCCCCGCGACTTGGGCCGAGGCGACCGGGCTGGTCGCGCGCCGGTTACGGGAG
GCCATCGCCCGCCACGGGCCGGACTCGGTCGCCTTCTACGTCTCGGGCCAGCTGCTGACCGAGGACTACTACGCCGCCAA
CAAGCTGATGAAGGGCTTCATCGGCTCGGCCAACATCGACACCAACTCGCGGCTGTGCATGGCCTCGGCGGTTGTGGCCC
ACAAGCTCGCCTTCGGCGCCGACCTCGTCCCGGGCTGCTACGAGGACCTGGACCTGGCCGACCTCGTGGTCCTGTCCGGA
CACAACGCCGCCTGGACCCACCCCGTGCTCTTCCGCCGCCTGGAAGCCGCCCGCGCCCGCGGCCAGCGCCATGTGGTGAT
CGACCCGCGCCGCACCGACACGGCCGAAGCCGCCGACCTGCACCTGCCCATCGCCCCGCAGACCGACGTGAGGCTGTGGA
ACGGCCTGCTGGCCGACCTCATCCGGCGGGGCGCGATCGACCTGGCGTTCCTCTCGGCCCACGTCGGGGGCTTCGCCGAG
ACCGAGGCCGAGCTCGCACGCGCCGACCAGTCGCCCGCCGCCGTCGCCGCCGACTGCGGCGTGCCGCTCGCCGACCTCGA
GCGCTTCTTCCGCTGGTTCGCCGCCACGCCGCGGACCGTCAGCCTCTTCTCCATGGGGGCCAACCAGTCGGCGCAGGGCG
TGGCCAAGGGCCTGGCCATCCTGAACGTCCACCTCGCCACCGGCCGCATCGGCAAGCCCGGGGCCTGCCCGTTCTCGATC
ACCGGCCAGCCCAACGCCATGGGCGGGCGCGAGACCGGCGGCATGGCCACCACGCTGGCCGCCCACATGGATTTCGAAGA
GGCCGCCCGCGCCCGGGTCCGGCGCTTCTGGGGCGCGCCGACGATCGCCGAGCGGCCCGGCCTGAGGGCCGTCGAGATGT
TCGAGGCGGTGCGCGAGGGCCGCATCAAGGCGCTGTGGATCATGGCCACCAATCCGGCGGTCAGCCTCCCCGACGCCGGC
CGCGTCCGCGAGGCGCTCGCCGCCTGTCCGTTCGTCGTGGTGTCCGACGTCATGGCCGCGACCGACACCTCGGCCTTCGC
CCACGTGCGCCTGCCCGCCCTGGCCTGGGGCGAGAAGGACGGGACCGTCACCAACTCCGAGCGCCGCATCTCGCGCCAGC
GGCCGCTGCTTCCCCCGCCCGGCGAGGCGCGCGCGGACTGGCGGATCGTCGCCGACGTGGCGACGGCCATGGGCTTCGGC
GAGGCCTTCGGCTGGCGCACGCCGGCGCAGGTGTTCCGCGAATGGGCGCGGCTGACCGCCTACGACAATCCGGACCGGGT
GCTGAACCTCGGCCCGCTCGCCGCCCTCACGCCCGAGGCCTACGACGCCCTGGAGCCGGTCCAGTGGCCGGTGACGGCCG
CCGGGGGCGCGCCGCGCCTGTTCACGGACGGGCGGTTCCAGACGCCTGACGGGCGGGGGCGCATGCATCCGGTGGCGGCC
GAGGGGCCGGCCGAGCCGGTGGATCCCGGCTATCCGCTGGCGCTCAACACCGGCCGGGTCCGCGACCACTGGCATACGCT
CACCCGCACCGGGCTGGCCCCCGAGCTGTGCCGCCATGCGCCCGAGCCCTATGTGGAGATCCACCCCGCCGACGCCGCGC
CCCTGGGGATCGCCGAGGGCGCGCTGACCCGGATCGTCACCCGGCAGGGCGAGGCCGTGGCGACCGCCCGCCTTTCCGAT
CGCCAGCGGCCGGGCTCGGTCTTCCTGCCGATGCACTGGACGCAGGCCTTCGCCCCGGCCGGCCGCGCCAACCCGCTGGT
CGCGGCGCGCACCGATCCCCGCTCGGGCCAGCCCGAGTTCAAGCACACGCCGGCCCGCGTGCGGCCCTATCGCGAGACCT
GGCGCGGCTTCCTGCTCGCCCCAGCGGCCGTCGAGCCGCCGCGAGGCTGCGACCTGGTCTGGCGGCGCATCCCACAGCCG
GCCTGCCAGCTGCACGAGTTCGCCGGCCGGGGGGACGGCGCCGAGCGCGAAGCGGTGCGCCGGGCGCTGGCCCGCGGCGC
GCCGGCCGAGGTGCTGCGCTTCGAGGACCCGGCCGCCGGCGTCCTGCGCGAGGCCTTCCTGGCGGAGGGGCGGCTCGAGC
GGGTGCTGTTCCTGGCGGCGGCCGGCGAGCTGCCGCCCCGGGACTGGCTGGCGGAGCTGTTCGCCGGCGGCGTGCTCGAC
CCCCTCCAGCGGGCGGCCCTGCTGGTCGGCCGCGCGCCCGGCCGCGCGGTGGAGGCCGGCCCCGTGGTCTGCGCCTGCCG
CGGCGTGCGCCAGCCGCGCATCGAGGCCGCCATCGCCGACGGGGCCCGCGACCTCGACGCCGTGGCCGGGGCGACCGGAG
CCGGCGCGTCCTGCGGCTCGTGCCGGCCCGAGATCGCCCGTCTCCTTTCCGCAACCAGTCCGCAGGTGCGCCATGCCGCC
TGA

Upstream 100 bases:

>100_bases
ACCTGGCCACCGGCGAGCCGCTGGGGGCCGACCGCGGCAAGGGCTGCGCGCCGGTGGCGCGGGTCCGGGTGGAGGGCGGT
CGCGTCCTCGTGGGGCGGGT

Downstream 100 bases:

>100_bases
TCCCGTTCCCGGCCGCGTCCGCCTAGTGGGCGCCGGCCCCGGCGCCGCCGACCTCCTGACCGTGCGCGCCCTGCGGGCGG
TGCAGTCGGCGCAGGCCCTG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MRTTCPYCGVGCGISAEAEGRALSVAGDPAHPANLGRLCSKGSALGATVGLEGRLLHPTIGDRPATWAEATGLVARRLRE
AIARHGPDSVAFYVSGQLLTEDYYAANKLMKGFIGSANIDTNSRLCMASAVVAHKLAFGADLVPGCYEDLDLADLVVLSG
HNAAWTHPVLFRRLEAARARGQRHVVIDPRRTDTAEAADLHLPIAPQTDVRLWNGLLADLIRRGAIDLAFLSAHVGGFAE
TEAELARADQSPAAVAADCGVPLADLERFFRWFAATPRTVSLFSMGANQSAQGVAKGLAILNVHLATGRIGKPGACPFSI
TGQPNAMGGRETGGMATTLAAHMDFEEAARARVRRFWGAPTIAERPGLRAVEMFEAVREGRIKALWIMATNPAVSLPDAG
RVREALAACPFVVVSDVMAATDTSAFAHVRLPALAWGEKDGTVTNSERRISRQRPLLPPPGEARADWRIVADVATAMGFG
EAFGWRTPAQVFREWARLTAYDNPDRVLNLGPLAALTPEAYDALEPVQWPVTAAGGAPRLFTDGRFQTPDGRGRMHPVAA
EGPAEPVDPGYPLALNTGRVRDHWHTLTRTGLAPELCRHAPEPYVEIHPADAAPLGIAEGALTRIVTRQGEAVATARLSD
RQRPGSVFLPMHWTQAFAPAGRANPLVAARTDPRSGQPEFKHTPARVRPYRETWRGFLLAPAAVEPPRGCDLVWRRIPQP
ACQLHEFAGRGDGAEREAVRRALARGAPAEVLRFEDPAAGVLREAFLAEGRLERVLFLAAAGELPPRDWLAELFAGGVLD
PLQRAALLVGRAPGRAVEAGPVVCACRGVRQPRIEAAIADGARDLDAVAGATGAGASCGSCRPEIARLLSATSPQVRHAA

Sequences:

>Translated_880_residues
MRTTCPYCGVGCGISAEAEGRALSVAGDPAHPANLGRLCSKGSALGATVGLEGRLLHPTIGDRPATWAEATGLVARRLRE
AIARHGPDSVAFYVSGQLLTEDYYAANKLMKGFIGSANIDTNSRLCMASAVVAHKLAFGADLVPGCYEDLDLADLVVLSG
HNAAWTHPVLFRRLEAARARGQRHVVIDPRRTDTAEAADLHLPIAPQTDVRLWNGLLADLIRRGAIDLAFLSAHVGGFAE
TEAELARADQSPAAVAADCGVPLADLERFFRWFAATPRTVSLFSMGANQSAQGVAKGLAILNVHLATGRIGKPGACPFSI
TGQPNAMGGRETGGMATTLAAHMDFEEAARARVRRFWGAPTIAERPGLRAVEMFEAVREGRIKALWIMATNPAVSLPDAG
RVREALAACPFVVVSDVMAATDTSAFAHVRLPALAWGEKDGTVTNSERRISRQRPLLPPPGEARADWRIVADVATAMGFG
EAFGWRTPAQVFREWARLTAYDNPDRVLNLGPLAALTPEAYDALEPVQWPVTAAGGAPRLFTDGRFQTPDGRGRMHPVAA
EGPAEPVDPGYPLALNTGRVRDHWHTLTRTGLAPELCRHAPEPYVEIHPADAAPLGIAEGALTRIVTRQGEAVATARLSD
RQRPGSVFLPMHWTQAFAPAGRANPLVAARTDPRSGQPEFKHTPARVRPYRETWRGFLLAPAAVEPPRGCDLVWRRIPQP
ACQLHEFAGRGDGAEREAVRRALARGAPAEVLRFEDPAAGVLREAFLAEGRLERVLFLAAAGELPPRDWLAELFAGGVLD
PLQRAALLVGRAPGRAVEAGPVVCACRGVRQPRIEAAIADGARDLDAVAGATGAGASCGSCRPEIARLLSATSPQVRHAA
>Mature_880_residues
MRTTCPYCGVGCGISAEAEGRALSVAGDPAHPANLGRLCSKGSALGATVGLEGRLLHPTIGDRPATWAEATGLVARRLRE
AIARHGPDSVAFYVSGQLLTEDYYAANKLMKGFIGSANIDTNSRLCMASAVVAHKLAFGADLVPGCYEDLDLADLVVLSG
HNAAWTHPVLFRRLEAARARGQRHVVIDPRRTDTAEAADLHLPIAPQTDVRLWNGLLADLIRRGAIDLAFLSAHVGGFAE
TEAELARADQSPAAVAADCGVPLADLERFFRWFAATPRTVSLFSMGANQSAQGVAKGLAILNVHLATGRIGKPGACPFSI
TGQPNAMGGRETGGMATTLAAHMDFEEAARARVRRFWGAPTIAERPGLRAVEMFEAVREGRIKALWIMATNPAVSLPDAG
RVREALAACPFVVVSDVMAATDTSAFAHVRLPALAWGEKDGTVTNSERRISRQRPLLPPPGEARADWRIVADVATAMGFG
EAFGWRTPAQVFREWARLTAYDNPDRVLNLGPLAALTPEAYDALEPVQWPVTAAGGAPRLFTDGRFQTPDGRGRMHPVAA
EGPAEPVDPGYPLALNTGRVRDHWHTLTRTGLAPELCRHAPEPYVEIHPADAAPLGIAEGALTRIVTRQGEAVATARLSD
RQRPGSVFLPMHWTQAFAPAGRANPLVAARTDPRSGQPEFKHTPARVRPYRETWRGFLLAPAAVEPPRGCDLVWRRIPQP
ACQLHEFAGRGDGAEREAVRRALARGAPAEVLRFEDPAAGVLREAFLAEGRLERVLFLAAAGELPPRDWLAELFAGGVLD
PLQRAALLVGRAPGRAVEAGPVVCACRGVRQPRIEAAIADGARDLDAVAGATGAGASCGSCRPEIARLLSATSPQVRHAA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=790, Percent_Identity=29.873417721519, Blast_Score=303, Evalue=3e-83,
Organism=Escherichia coli, GI3868721, Length=704, Percent_Identity=27.6988636363636, Blast_Score=282, Evalue=6e-77,
Organism=Escherichia coli, GI3868719, Length=614, Percent_Identity=23.7785016286645, Blast_Score=143, Evalue=5e-35,
Organism=Escherichia coli, GI3868720, Length=612, Percent_Identity=23.6928104575163, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1787778, Length=693, Percent_Identity=22.7994227994228, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081797, Length=731, Percent_Identity=22.7086183310534, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI1787870, Length=720, Percent_Identity=22.6388888888889, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI87081994, Length=354, Percent_Identity=26.8361581920904, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93772; Mature: 93772

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTCPYCGVGCGISAEAEGRALSVAGDPAHPANLGRLCSKGSALGATVGLEGRLLHPTI
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCEEECCCC
GDRPATWAEATGLVARRLREAIARHGPDSVAFYVSGQLLTEDYYAANKLMKGFIGSANID
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHCCCCCCC
TNSRLCMASAVVAHKLAFGADLVPGCYEDLDLADLVVLSGHNAAWTHPVLFRRLEAARAR
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
GQRHVVIDPRRTDTAEAADLHLPIAPQTDVRLWNGLLADLIRRGAIDLAFLSAHVGGFAE
CCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
TEAELARADQSPAAVAADCGVPLADLERFFRWFAATPRTVSLFSMGANQSAQGVAKGLAI
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCEEE
LNVHLATGRIGKPGACPFSITGQPNAMGGRETGGMATTLAAHMDFEEAARARVRRFWGAP
EEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
TIAERPGLRAVEMFEAVREGRIKALWIMATNPAVSLPDAGRVREALAACPFVVVSDVMAA
CCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHH
TDTSAFAHVRLPALAWGEKDGTVTNSERRISRQRPLLPPPGEARADWRIVADVATAMGFG
CCCCCEEEEECCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EAFGWRTPAQVFREWARLTAYDNPDRVLNLGPLAALTPEAYDALEPVQWPVTAAGGAPRL
HHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCHHHHHCCCCCCCEEECCCCCCE
FTDGRFQTPDGRGRMHPVAAEGPAEPVDPGYPLALNTGRVRDHWHTLTRTGLAPELCRHA
ECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHCC
PEPYVEIHPADAAPLGIAEGALTRIVTRQGEAVATARLSDRQRPGSVFLPMHWTQAFAPA
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHCCC
GRANPLVAARTDPRSGQPEFKHTPARVRPYRETWRGFLLAPAAVEPPRGCDLVWRRIPQP
CCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHCCHH
ACQLHEFAGRGDGAEREAVRRALARGAPAEVLRFEDPAAGVLREAFLAEGRLERVLFLAA
HHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHCCHHHHEEEEEE
AGELPPRDWLAELFAGGVLDPLQRAALLVGRAPGRAVEAGPVVCACRGVRQPRIEAAIAD
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHC
GARDLDAVAGATGAGASCGSCRPEIARLLSATSPQVRHAA
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MRTTCPYCGVGCGISAEAEGRALSVAGDPAHPANLGRLCSKGSALGATVGLEGRLLHPTI
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCEEECCCC
GDRPATWAEATGLVARRLREAIARHGPDSVAFYVSGQLLTEDYYAANKLMKGFIGSANID
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEHHHHHHHHHHHHHHCCCCCCC
TNSRLCMASAVVAHKLAFGADLVPGCYEDLDLADLVVLSGHNAAWTHPVLFRRLEAARAR
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
GQRHVVIDPRRTDTAEAADLHLPIAPQTDVRLWNGLLADLIRRGAIDLAFLSAHVGGFAE
CCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
TEAELARADQSPAAVAADCGVPLADLERFFRWFAATPRTVSLFSMGANQSAQGVAKGLAI
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCEEE
LNVHLATGRIGKPGACPFSITGQPNAMGGRETGGMATTLAAHMDFEEAARARVRRFWGAP
EEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
TIAERPGLRAVEMFEAVREGRIKALWIMATNPAVSLPDAGRVREALAACPFVVVSDVMAA
CCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHH
TDTSAFAHVRLPALAWGEKDGTVTNSERRISRQRPLLPPPGEARADWRIVADVATAMGFG
CCCCCEEEEECCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EAFGWRTPAQVFREWARLTAYDNPDRVLNLGPLAALTPEAYDALEPVQWPVTAAGGAPRL
HHCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCHHHHHCCCCCCCEEECCCCCCE
FTDGRFQTPDGRGRMHPVAAEGPAEPVDPGYPLALNTGRVRDHWHTLTRTGLAPELCRHA
ECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHCC
PEPYVEIHPADAAPLGIAEGALTRIVTRQGEAVATARLSDRQRPGSVFLPMHWTQAFAPA
CCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHCCC
GRANPLVAARTDPRSGQPEFKHTPARVRPYRETWRGFLLAPAAVEPPRGCDLVWRRIPQP
CCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHCCHH
ACQLHEFAGRGDGAEREAVRRALARGAPAEVLRFEDPAAGVLREAFLAEGRLERVLFLAA
HHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHCCHHHHEEEEEE
AGELPPRDWLAELFAGGVLDPLQRAALLVGRAPGRAVEAGPVVCACRGVRQPRIEAAIAD
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHC
GARDLDAVAGATGAGASCGSCRPEIARLLSATSPQVRHAA
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]