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The map label for this gene is apbE [H]

Identifier: 197104274

GI number: 197104274

Start: 892679

End: 893665

Strand: Direct

Name: apbE [H]

Synonym: PHZ_c0808

Alternate gene names: 197104274

Gene position: 892679-893665 (Clockwise)

Preceding gene: 197104273

Following gene: 197104275

Centisome position: 22.34

GC content: 76.9

Gene sequence:

>987_bases
ATGCGCCGCGTCGCGATCCCCCTCGATCTGACGCCCGAGGCCGCCCGTCCCCGCGAGGGGCGGGCGGTTTCGCTGTCCGG
TCCCACCATGGGCGTCGCCTGGACGCTGAAGGCGCGTGCGCCGGCCGGGCTGTCGGACGCCGCGGTCGCGGCGGCCGTGC
AGGGCGCCTGCGACCGCGTGGTCGCCGAGATGAGCCAGTGGGAGCCGGGCAGCGACCTTACCCGTTTCAACCGCGCCCCG
GCGGGAAGCTGGACCGACCTGCCGCCCGACTTCGCGACGGTGATGGAGGCGGCCCTGGAGCTGGCGCGGACCAGTGACGG
CGCCTTCGATCCGACCCTGGGCGACCTCGTGGACCTGTGGGGCTTCGGGCCCGCCGGGCCAGCCGCGACGATCCCCGACC
CTACGGCGCTGCGGGCGGCCCGCGCCGGCTGGGCGGACCTGCGCCTGGACGCGGGGCGGCTGTTCCAGCCGGGAGGCGTG
GCCCTTGACCTGTCCGGCGTCGCCAAGGGCTACGGCGTCGACCTCGCCGCTGCGGCGCTGCGAGGGCTGGGCGTGCGCGA
CTTCCTGCTCGAGATCGGCGGCGAGCTGCGCGGGGAAGGGGTGAAGGGCGACGGCGAGCCCTGGTGGGTGGAGGTCGAGC
GGCCGCCGGACGCGGATCTGGCCGAGCCGCCGGTGCTCGTCGCCCTGCACGGCCTCTCCGTCGCCACCTCCGGCGACTGG
CGCCGCGCCTTCGTCTCGGACGGGCGCCGCTACGCCCACACCCTCGATCCCGCGACGCGCGCGCCGCTCCCCGCGGACGT
GGCCGCCGCCACGGTGGTCCATCGCGACTGCCTGCACGCCGACGCGCTCTGCACCGCGCTGCTGGTGCTGGGCTCCCGCG
CCGAGGCCTTCGCGCGCGAGCACGACGTCGCCGCGCTGATCCTGCGCCGCGCGGCGGGGGGCTACGAGGAAGTCCTCACC
CCCGCCTTCGAGCGCCTGCTGGCCTGA

Upstream 100 bases:

>100_bases
TCTATTGGCTGGAAGCGGGCGTCCAGGGCCCCGGCGAGAACGGCCAGCGCCGCAGCCTTGGCTATGTCGCCACCTTCGAG
GTCCTGCCCGACTAGGAGCC

Downstream 100 bases:

>100_bases
GGCGCGCGCGAGGTTGCGCGACGTCAGTCTTGCGGCGGCGGCGGGCCGGCTCTACCGTCCGTTCAAACAAGTGTTGGGAG
GACCTCCAATGACCATCGGT

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRRVAIPLDLTPEAARPREGRAVSLSGPTMGVAWTLKARAPAGLSDAAVAAAVQGACDRVVAEMSQWEPGSDLTRFNRAP
AGSWTDLPPDFATVMEAALELARTSDGAFDPTLGDLVDLWGFGPAGPAATIPDPTALRAARAGWADLRLDAGRLFQPGGV
ALDLSGVAKGYGVDLAAAALRGLGVRDFLLEIGGELRGEGVKGDGEPWWVEVERPPDADLAEPPVLVALHGLSVATSGDW
RRAFVSDGRRYAHTLDPATRAPLPADVAAATVVHRDCLHADALCTALLVLGSRAEAFAREHDVAALILRRAAGGYEEVLT
PAFERLLA

Sequences:

>Translated_328_residues
MRRVAIPLDLTPEAARPREGRAVSLSGPTMGVAWTLKARAPAGLSDAAVAAAVQGACDRVVAEMSQWEPGSDLTRFNRAP
AGSWTDLPPDFATVMEAALELARTSDGAFDPTLGDLVDLWGFGPAGPAATIPDPTALRAARAGWADLRLDAGRLFQPGGV
ALDLSGVAKGYGVDLAAAALRGLGVRDFLLEIGGELRGEGVKGDGEPWWVEVERPPDADLAEPPVLVALHGLSVATSGDW
RRAFVSDGRRYAHTLDPATRAPLPADVAAATVVHRDCLHADALCTALLVLGSRAEAFAREHDVAALILRRAAGGYEEVLT
PAFERLLA
>Mature_328_residues
MRRVAIPLDLTPEAARPREGRAVSLSGPTMGVAWTLKARAPAGLSDAAVAAAVQGACDRVVAEMSQWEPGSDLTRFNRAP
AGSWTDLPPDFATVMEAALELARTSDGAFDPTLGDLVDLWGFGPAGPAATIPDPTALRAARAGWADLRLDAGRLFQPGGV
ALDLSGVAKGYGVDLAAAALRGLGVRDFLLEIGGELRGEGVKGDGEPWWVEVERPPDADLAEPPVLVALHGLSVATSGDW
RRAFVSDGRRYAHTLDPATRAPLPADVAAATVVHRDCLHADALCTALLVLGSRAEAFAREHDVAALILRRAAGGYEEVLT
PAFERLLA

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=327, Percent_Identity=29.3577981651376, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34339; Mature: 34339

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVAIPLDLTPEAARPREGRAVSLSGPTMGVAWTLKARAPAGLSDAAVAAAVQGACDRV
CCEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
VAEMSQWEPGSDLTRFNRAPAGSWTDLPPDFATVMEAALELARTSDGAFDPTLGDLVDLW
HHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGPAGPAATIPDPTALRAARAGWADLRLDAGRLFQPGGVALDLSGVAKGYGVDLAAAAL
CCCCCCCCCCCCCHHHHHHHHCCCHHEEECCCCEECCCCEEEECCCCCCCCCHHHHHHHH
RGLGVRDFLLEIGGELRGEGVKGDGEPWWVEVERPPDADLAEPPVLVALHGLSVATSGDW
HCCCHHHHHHHHCCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCEEECCCCH
RRAFVSDGRRYAHTLDPATRAPLPADVAAATVVHRDCLHADALCTALLVLGSRAEAFARE
HHHHHHCCCCEEHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HDVAALILRRAAGGYEEVLTPAFERLLA
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRVAIPLDLTPEAARPREGRAVSLSGPTMGVAWTLKARAPAGLSDAAVAAAVQGACDRV
CCEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
VAEMSQWEPGSDLTRFNRAPAGSWTDLPPDFATVMEAALELARTSDGAFDPTLGDLVDLW
HHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGPAGPAATIPDPTALRAARAGWADLRLDAGRLFQPGGVALDLSGVAKGYGVDLAAAAL
CCCCCCCCCCCCCHHHHHHHHCCCHHEEECCCCEECCCCEEEECCCCCCCCCHHHHHHHH
RGLGVRDFLLEIGGELRGEGVKGDGEPWWVEVERPPDADLAEPPVLVALHGLSVATSGDW
HCCCHHHHHHHHCCHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCEEECCCCH
RRAFVSDGRRYAHTLDPATRAPLPADVAAATVVHRDCLHADALCTALLVLGSRAEAFARE
HHHHHHCCCCEEHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HDVAALILRRAAGGYEEVLTPAFERLLA
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]