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The map label for this gene is ispB [H]

Identifier: 197104247

GI number: 197104247

Start: 860883

End: 861890

Strand: Direct

Name: ispB [H]

Synonym: PHZ_c0781

Alternate gene names: 197104247

Gene position: 860883-861890 (Clockwise)

Preceding gene: 197104245

Following gene: 197104248

Centisome position: 21.54

GC content: 73.51

Gene sequence:

>1008_bases
TTGGACGCCGCCCAGGCCATCGCTGCACCGAAGCCCTCCATCGACGCGCTGATGGCGCTGGCCGGCCCCGACATGGCGGC
CGTCGACGCCCTGATCCGCGAGCACATGGACAGTCCGGTGCCGGTGATCCCGGCGCTGGCCGACCACCTGATCGCCGGCT
CGGCCAAGCGGTTGCGGCCGCTGCTCACCATCGCCGCGGCCAGGCTGGCCGGCGCGCGGGACGACTCCTGCCTGAAGCTG
GCCGCCGCCGTGGAGTTCATCCACACGGCCACCCTGCTGCACGACGACGTGGTGGATTCGTCCCAGCTGCGCCGCGGCCG
CGTGGCCGCCCACCTGATCTGGGGCGCGCCGTCCAGCGTGCTGGTCGGCGACTTCCTGTTCGCCCGGGCCTTCGAGCTGA
TGGTCGGCGCCGGCTCGATGGCCGCGCTGGAGATCCTGGCCCGCGCCAGCCGGGTGATCTCCGAGGGCGAGGTGCTGCAG
CTCACCCGCAGCCACGACCTCGACCTCACCCAGGAGGTCTACATCGAGATCATCGGGGCCAAGACCGCCGAGCTGTTCGC
CGCCGCCGCCGAGGCCGGCGCGGTGGCGGCCGGAGCTTCGCCCGAGCGCTGCCGGGCCCTGCGCCGCTTCGGCCAGGACC
TGGGCTTGGCCTTCCAGCTCGTCGACGACGCCCTCGACTATTCCGGCGACGCCAAGACCCTGGGCAAGAACGCCGGCGAC
GACTTCCGGGAGGGCAAGGTCACCCTGCCGCTGCTCTACGCCATGGCCCGCACGGGCGCGGCCGAGCGCGACTTCTGGCA
GCGCACCGTCGACCGCCGCGAGCAGACCGAGGCCGACTTCGACCGCGCCCGCGAGCTGATGGGCCAGACCCGGGCGCTCG
ACGACACCCTCGCCCTGGCCGCCCGCTACGCCGACAGCGCCAAGGCCGCGCTCGCCGACTTTCCGTCGAACAGCTGGCGC
CCGGCGCTCGAGGACCTGGCGGACTTCTCGGTGGCCCGGAAGGCCTAG

Upstream 100 bases:

>100_bases
ACGCTTGCGGCTCGCTTGACCGTGCCCTATCGCGTCACCATTGTCCCGGCAAACGGCTACCGGTCGGGGCCCGGACGCCT
TGTCGCCAAAGGAATTCCGC

Downstream 100 bases:

>100_bases
GGAAAGCAGCGCCTTAGGAACCTAAGCCGGTCTCGCGCGCTCCCTCCGCCCCGAGGGAGTGACGATGTTTCCTGGCATGA
GACTGGCCCGCCTGGGCGCC

Product: polyprenyl synthetase family protein

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MDAAQAIAAPKPSIDALMALAGPDMAAVDALIREHMDSPVPVIPALADHLIAGSAKRLRPLLTIAAARLAGARDDSCLKL
AAAVEFIHTATLLHDDVVDSSQLRRGRVAAHLIWGAPSSVLVGDFLFARAFELMVGAGSMAALEILARASRVISEGEVLQ
LTRSHDLDLTQEVYIEIIGAKTAELFAAAAEAGAVAAGASPERCRALRRFGQDLGLAFQLVDDALDYSGDAKTLGKNAGD
DFREGKVTLPLLYAMARTGAAERDFWQRTVDRREQTEADFDRARELMGQTRALDDTLALAARYADSAKAALADFPSNSWR
PALEDLADFSVARKA

Sequences:

>Translated_335_residues
MDAAQAIAAPKPSIDALMALAGPDMAAVDALIREHMDSPVPVIPALADHLIAGSAKRLRPLLTIAAARLAGARDDSCLKL
AAAVEFIHTATLLHDDVVDSSQLRRGRVAAHLIWGAPSSVLVGDFLFARAFELMVGAGSMAALEILARASRVISEGEVLQ
LTRSHDLDLTQEVYIEIIGAKTAELFAAAAEAGAVAAGASPERCRALRRFGQDLGLAFQLVDDALDYSGDAKTLGKNAGD
DFREGKVTLPLLYAMARTGAAERDFWQRTVDRREQTEADFDRARELMGQTRALDDTLALAARYADSAKAALADFPSNSWR
PALEDLADFSVARKA
>Mature_335_residues
MDAAQAIAAPKPSIDALMALAGPDMAAVDALIREHMDSPVPVIPALADHLIAGSAKRLRPLLTIAAARLAGARDDSCLKL
AAAVEFIHTATLLHDDVVDSSQLRRGRVAAHLIWGAPSSVLVGDFLFARAFELMVGAGSMAALEILARASRVISEGEVLQ
LTRSHDLDLTQEVYIEIIGAKTAELFAAAAEAGAVAAGASPERCRALRRFGQDLGLAFQLVDDALDYSGDAKTLGKNAGD
DFREGKVTLPLLYAMARTGAAERDFWQRTVDRREQTEADFDRARELMGQTRALDDTLALAARYADSAKAALADFPSNSWR
PALEDLADFSVARKA

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=317, Percent_Identity=27.1293375394322, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758430, Length=208, Percent_Identity=25.9615384615385, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI83700220, Length=208, Percent_Identity=25.9615384615385, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=44.2367601246106, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1786623, Length=242, Percent_Identity=32.6446280991736, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=243, Percent_Identity=32.0987654320988, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=31.2292358803987, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325188, Length=258, Percent_Identity=27.1317829457364, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24651612, Length=301, Percent_Identity=27.2425249169435, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35733; Mature: 35733

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAQAIAAPKPSIDALMALAGPDMAAVDALIREHMDSPVPVIPALADHLIAGSAKRLRP
CCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
LLTIAAARLAGARDDSCLKLAAAVEFIHTATLLHDDVVDSSQLRRGRVAAHLIWGAPSSV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCHH
LVGDFLFARAFELMVGAGSMAALEILARASRVISEGEVLQLTRSHDLDLTQEVYIEIIGA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCC
KTAELFAAAAEAGAVAAGASPERCRALRRFGQDLGLAFQLVDDALDYSGDAKTLGKNAGD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
DFREGKVTLPLLYAMARTGAAERDFWQRTVDRREQTEADFDRARELMGQTRALDDTLALA
CHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYADSAKAALADFPSNSWRPALEDLADFSVARKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAAQAIAAPKPSIDALMALAGPDMAAVDALIREHMDSPVPVIPALADHLIAGSAKRLRP
CCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
LLTIAAARLAGARDDSCLKLAAAVEFIHTATLLHDDVVDSSQLRRGRVAAHLIWGAPSSV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCHH
LVGDFLFARAFELMVGAGSMAALEILARASRVISEGEVLQLTRSHDLDLTQEVYIEIIGA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCC
KTAELFAAAAEAGAVAAGASPERCRALRRFGQDLGLAFQLVDDALDYSGDAKTLGKNAGD
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
DFREGKVTLPLLYAMARTGAAERDFWQRTVDRREQTEADFDRARELMGQTRALDDTLALA
CHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYADSAKAALADFPSNSWRPALEDLADFSVARKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]