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The map label for this gene is narI [H]

Identifier: 197104154

GI number: 197104154

Start: 750933

End: 751724

Strand: Direct

Name: narI [H]

Synonym: PHZ_c0688

Alternate gene names: 197104154

Gene position: 750933-751724 (Clockwise)

Preceding gene: 197104153

Following gene: 197104155

Centisome position: 18.79

GC content: 68.43

Gene sequence:

>792_bases
ATGGAGTCCTTCATCAATCAGCTCGCCTTCGGCTGGTTTCCCTACCTGGCGGTGACGGTGCTGATCGTCGGCAGCATCTT
CCGCTTCGACAAGGCGCAGTACACTTGGCGCTCGCAGTCCAGCCAGTTCCTGCGGCGACGCCAGATGCTGATCGGCTCGA
ACCTGTTCCACATGGGCGTGCTGGTGCTGCTGGGCGGCCACTTCGTGGGGCTGCTCACGCCGATCAACGTGTTCGACGCC
CTGGGCGTGCAGCACGGCTTCAAGCAGATGGCGGCGCTGGTGGTCGGCGGCATCGCCGGGATCCTGGCCTGGGTCGGGGC
CAGCCTGCTGCTGCATCGCCGGCTGTTCGACTCCCGAATCCGCCGCAGCTCGCACTGGACCGACATCGCCGTCCTGGTGC
TGCTCTGGCTGCAGCTCACCCTGGGCCTGGCCACCACCTGGTGGACCATGAAGCACCTCGACGGCGAGGAGATGCTGCTC
TTCATGGGCTGGGCCAACGGCCTGCTCACCTTCAACCCGCAGGCGGCCGACCTGATCGTGGACGCCGCCCTGGTCTACAA
GCTGCACATCATCCTGGGCCTGACGCTGTTCCTCGTGACCCCGTTCAGCCGGCTGGTCCACATCTGGAGCATCCCGCTCT
TCTTCCTGCTGCGGCCGGGCTACCAGATCGTGCGGACCCGCGGACCGGTGCGCCGGCCGCAGCCCGCGTCGCTGTCGCAC
GGCCCCGCCGGCGCGGCGCCGACCTATGGCGGCCCGGCGCCGCAGCGCGCCGCCGCCGAGGAATTCCAGTGA

Upstream 100 bases:

>100_bases
GCCCGAGGCGCCCCAGCCCACGGGCTTCGTCGCCAAACTCCGGGCCTGGAAACGGCCCGCCGAGCCGGCCCCGCCGGCCG
CATCCTGAGGGAGCCGCGTC

Downstream 100 bases:

>100_bases
GCGAGGCCCGCTTCGTCGTCACGCGCTCGGCCGGGCGCGGCGCCGCCCGGCCGGCGCCTCCGCCTGCTCCCGAGGCCGTT
TCCCAGGCCGTTCCCCAGGC

Product: NarI, respiratory nitrate reductase, gamma subunit

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MESFINQLAFGWFPYLAVTVLIVGSIFRFDKAQYTWRSQSSQFLRRRQMLIGSNLFHMGVLVLLGGHFVGLLTPINVFDA
LGVQHGFKQMAALVVGGIAGILAWVGASLLLHRRLFDSRIRRSSHWTDIAVLVLLWLQLTLGLATTWWTMKHLDGEEMLL
FMGWANGLLTFNPQAADLIVDAALVYKLHIILGLTLFLVTPFSRLVHIWSIPLFFLLRPGYQIVRTRGPVRRPQPASLSH
GPAGAAPTYGGPAPQRAAAEEFQ

Sequences:

>Translated_263_residues
MESFINQLAFGWFPYLAVTVLIVGSIFRFDKAQYTWRSQSSQFLRRRQMLIGSNLFHMGVLVLLGGHFVGLLTPINVFDA
LGVQHGFKQMAALVVGGIAGILAWVGASLLLHRRLFDSRIRRSSHWTDIAVLVLLWLQLTLGLATTWWTMKHLDGEEMLL
FMGWANGLLTFNPQAADLIVDAALVYKLHIILGLTLFLVTPFSRLVHIWSIPLFFLLRPGYQIVRTRGPVRRPQPASLSH
GPAGAAPTYGGPAPQRAAAEEFQ
>Mature_263_residues
MESFINQLAFGWFPYLAVTVLIVGSIFRFDKAQYTWRSQSSQFLRRRQMLIGSNLFHMGVLVLLGGHFVGLLTPINVFDA
LGVQHGFKQMAALVVGGIAGILAWVGASLLLHRRLFDSRIRRSSHWTDIAVLVLLWLQLTLGLATTWWTMKHLDGEEMLL
FMGWANGLLTFNPQAADLIVDAALVYKLHIILGLTLFLVTPFSRLVHIWSIPLFFLLRPGYQIVRTRGPVRRPQPASLSH
GPAGAAPTYGGPAPQRAAAEEFQ

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=226, Percent_Identity=45.1327433628319, Blast_Score=196, Evalue=9e-52,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=42.2907488986784, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 29372; Mature: 29372

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]