Click here to switch to the map view.

The map label for this gene is narH [H]

Identifier: 197104152

GI number: 197104152

Start: 748699

End: 750225

Strand: Direct

Name: narH [H]

Synonym: PHZ_c0686

Alternate gene names: 197104152

Gene position: 748699-750225 (Clockwise)

Preceding gene: 197104151

Following gene: 197104153

Centisome position: 18.74

GC content: 66.86

Gene sequence:

>1527_bases
ATGAAGGTCCGCGCGCAAATCTGCATGGTGCTGAACCTCGACAAGTGCATCGGGTGCCACACCTGCTCGATCACCTGCAA
GAACGTGTGGACCAACCGCGAAGGCGTCGAATACGTCTGGTTCAACAACGTCGAGAGCAAGCCGGGCGTCGGCTATCCGA
AGGACTGGGAGAACCAGTCCCGCTGGAAGGGCGGCTGGGTCCGCAAGCCCAATGGCCGGCTGGAGCCCCGCATCGGCGCC
AAATGGCGGATCCTGGCGAAGATCTTCGCCAACCCCGACCTGCCCGAGATCGACGACTTCTACGAGCCCTACACCTTCGA
ATACGAGTGGCTGGAGAAGGCCCCCGAGCTGCAGGCCCAGCCGACCGCCAAGCCGCGCTCGCTGATCACCGGCGAGGTGA
TCAAGAAGGTGGAGTGGAGCGGGAACTGGGAGGACGACCTCGGCGGCGAGTTCTCCAAGCGCTCCCAGGACTACAATTTC
GAGGGCGTCCAGAAGGAGATCTACGGCCAGTTCGAGAACACCTTCCTGATGTACCTGCCCAGGCTGTGCGAGCACTGCCT
GAACCCCTCGTGCCTGGCCGCATGTCCCTCGGGGGCGATCTACAAGCGGGCCGAGGACGGCATTGTCCTCATCGACCAGG
AACGCTGCCGCGGCTGGCGGATGTGCGTCTCGGGATGCCCGTACAAGAAGGTCTATTACAACTGGTCGTCGGGTAAGTCG
GAGAAGTGCATCTTCTGCTTCCCGCGGATCGAGGCGGGCCAGCCGACGGTGTGCTCCGAGACCTGCGTCGGCCGCATCCG
CTACCTGGGCGTGATCCTCTACGACGCCGACCGCATCAGCGAGGCGGCGAGCGTGGAGCGGGAGGAGGACCTCTATCCCG
CGCAGTTGTCGGTGTTCCTCGACCCCAACGACCCGGCGGTGCAGGAGCAGGCCCGCCGGGACGGCGTGCCCGAGACCTGG
ATCGAGGCGGCGACCCGCTCGCCGGTCTGGAAGATGGCGTGCGAGTGGAAGGTCGCCTTCCCGCTGCACCCCGAGTACCG
GACCCTGCCGATGGTCTGGTACGTGCCGCCGCTGTCGCCGATCCAGTCCGCGGCCGAGGCCGGCAAGATGACGGTCAACA
ACGGCATGCCGGACGTGCGCTCGCTGCGCATCCCGCTTCGGTACCTCGCCAACCTGCTCACCGCCGGCGCCGAGGAGCCG
GTGGCCCAGGCGCTGGAGCGGATGCTGGCCATGCGGGCCTACATGCGGGCCAAGACGGTCGACGGCGTCGTCGACGAGGG
CATCGCCCAGCAGGTCGGCCTGACCGGCGCGGTGATCGAGGAGATGTACCGCTACATGGGGCTGGCGGCCTACGAGGACC
GCTACGTCATCCCGACCGCCCACCGGGAGGACACCGAGGACGCCTACATGCTGCGCGGCGCCGCCGGCTTCGCGTTCCGG
GAAGGCACCAACGGCACGACCAAGGCCGACCTGTTCGGCCGCCGCGACCGCACGCCCCGCAAGAAGTACATGGACATCCC
GACATGA

Upstream 100 bases:

>100_bases
TCGGCGCCAACCGGGACGAGTTCATCATCGTGCGGAAGATGAACCAGCTGAACTGGTTCGACAAAGCCGCCGCCCAGGTC
GAGCAGGAGGCGAAGGTCTG

Downstream 100 bases:

>100_bases
GACGGACGCTGAAGGCTCTCTCGCTCCTGCTGTCGTATCCGACCGAGGAGCTGAAGGCGGGCGCGGGCGAGTTGCGCGCG
GCGCTGGCCGACGAGGCCCT

Product: respiratory nitrate reductase, beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKVRAQICMVLNLDKCIGCHTCSITCKNVWTNREGVEYVWFNNVESKPGVGYPKDWENQSRWKGGWVRKPNGRLEPRIGA
KWRILAKIFANPDLPEIDDFYEPYTFEYEWLEKAPELQAQPTAKPRSLITGEVIKKVEWSGNWEDDLGGEFSKRSQDYNF
EGVQKEIYGQFENTFLMYLPRLCEHCLNPSCLAACPSGAIYKRAEDGIVLIDQERCRGWRMCVSGCPYKKVYYNWSSGKS
EKCIFCFPRIEAGQPTVCSETCVGRIRYLGVILYDADRISEAASVEREEDLYPAQLSVFLDPNDPAVQEQARRDGVPETW
IEAATRSPVWKMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGKMTVNNGMPDVRSLRIPLRYLANLLTAGAEEP
VAQALERMLAMRAYMRAKTVDGVVDEGIAQQVGLTGAVIEEMYRYMGLAAYEDRYVIPTAHREDTEDAYMLRGAAGFAFR
EGTNGTTKADLFGRRDRTPRKKYMDIPT

Sequences:

>Translated_508_residues
MKVRAQICMVLNLDKCIGCHTCSITCKNVWTNREGVEYVWFNNVESKPGVGYPKDWENQSRWKGGWVRKPNGRLEPRIGA
KWRILAKIFANPDLPEIDDFYEPYTFEYEWLEKAPELQAQPTAKPRSLITGEVIKKVEWSGNWEDDLGGEFSKRSQDYNF
EGVQKEIYGQFENTFLMYLPRLCEHCLNPSCLAACPSGAIYKRAEDGIVLIDQERCRGWRMCVSGCPYKKVYYNWSSGKS
EKCIFCFPRIEAGQPTVCSETCVGRIRYLGVILYDADRISEAASVEREEDLYPAQLSVFLDPNDPAVQEQARRDGVPETW
IEAATRSPVWKMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGKMTVNNGMPDVRSLRIPLRYLANLLTAGAEEP
VAQALERMLAMRAYMRAKTVDGVVDEGIAQQVGLTGAVIEEMYRYMGLAAYEDRYVIPTAHREDTEDAYMLRGAAGFAFR
EGTNGTTKADLFGRRDRTPRKKYMDIPT
>Mature_508_residues
MKVRAQICMVLNLDKCIGCHTCSITCKNVWTNREGVEYVWFNNVESKPGVGYPKDWENQSRWKGGWVRKPNGRLEPRIGA
KWRILAKIFANPDLPEIDDFYEPYTFEYEWLEKAPELQAQPTAKPRSLITGEVIKKVEWSGNWEDDLGGEFSKRSQDYNF
EGVQKEIYGQFENTFLMYLPRLCEHCLNPSCLAACPSGAIYKRAEDGIVLIDQERCRGWRMCVSGCPYKKVYYNWSSGKS
EKCIFCFPRIEAGQPTVCSETCVGRIRYLGVILYDADRISEAASVEREEDLYPAQLSVFLDPNDPAVQEQARRDGVPETW
IEAATRSPVWKMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGKMTVNNGMPDVRSLRIPLRYLANLLTAGAEEP
VAQALERMLAMRAYMRAKTVDGVVDEGIAQQVGLTGAVIEEMYRYMGLAAYEDRYVIPTAHREDTEDAYMLRGAAGFAFR
EGTNGTTKADLFGRRDRTPRKKYMDIPT

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=497, Percent_Identity=68.41046277666, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=494, Percent_Identity=67.4089068825911, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=170, Percent_Identity=32.9411764705882, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=34.8484848484849, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=36.7816091954023, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789370, Length=107, Percent_Identity=36.4485981308411, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=101, Percent_Identity=38.6138613861386, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=34.4444444444444, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57943; Mature: 57943

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRAQICMVLNLDKCIGCHTCSITCKNVWTNREGVEYVWFNNVESKPGVGYPKDWENQS
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RWKGGWVRKPNGRLEPRIGAKWRILAKIFANPDLPEIDDFYEPYTFEYEWLEKAPELQAQ
CCCCCCEECCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCEEHHHHHCCCCCCCC
PTAKPRSLITGEVIKKVEWSGNWEDDLGGEFSKRSQDYNFEGVQKEIYGQFENTFLMYLP
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCLAACPSGAIYKRAEDGIVLIDQERCRGWRMCVSGCPYKKVYYNWSSGKS
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEEHHHCCHHHHHHCCCCCCEEEEECCCCCC
EKCIFCFPRIEAGQPTVCSETCVGRIRYLGVILYDADRISEAASVEREEDLYPAQLSVFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHCCCCCEEEEEEE
DPNDPAVQEQARRDGVPETWIEAATRSPVWKMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAAEAGKMTVNNGMPDVRSLRIPLRYLANLLTAGAEEPVAQALERMLAMRAYMRAKTV
HHHHHHHCCEEECCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DGVVDEGIAQQVGLTGAVIEEMYRYMGLAAYEDRYVIPTAHREDTEDAYMLRGAAGFAFR
HHHHHHHHHHHHCCHHHHHHHHHHHHCEEEECCCEEECCCCCCCCCCCEEEECCCCCEEE
EGTNGTTKADLFGRRDRTPRKKYMDIPT
CCCCCCCHHHHCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MKVRAQICMVLNLDKCIGCHTCSITCKNVWTNREGVEYVWFNNVESKPGVGYPKDWENQS
CCCCEEEEEEEEHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RWKGGWVRKPNGRLEPRIGAKWRILAKIFANPDLPEIDDFYEPYTFEYEWLEKAPELQAQ
CCCCCCEECCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHHCCCCCEEHHHHHCCCCCCCC
PTAKPRSLITGEVIKKVEWSGNWEDDLGGEFSKRSQDYNFEGVQKEIYGQFENTFLMYLP
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCLAACPSGAIYKRAEDGIVLIDQERCRGWRMCVSGCPYKKVYYNWSSGKS
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEEHHHCCHHHHHHCCCCCCEEEEECCCCCC
EKCIFCFPRIEAGQPTVCSETCVGRIRYLGVILYDADRISEAASVEREEDLYPAQLSVFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHCCCCCEEEEEEE
DPNDPAVQEQARRDGVPETWIEAATRSPVWKMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAAEAGKMTVNNGMPDVRSLRIPLRYLANLLTAGAEEPVAQALERMLAMRAYMRAKTV
HHHHHHHCCEEECCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DGVVDEGIAQQVGLTGAVIEEMYRYMGLAAYEDRYVIPTAHREDTEDAYMLRGAAGFAFR
HHHHHHHHHHHHCCHHHHHHHHHHHHCEEEECCCEEECCCCCCCCCCCEEEECCCCCEEE
EGTNGTTKADLFGRRDRTPRKKYMDIPT
CCCCCCCHHHHCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]