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The map label for this gene is narK [H]

Identifier: 197104150

GI number: 197104150

Start: 742218

End: 744965

Strand: Direct

Name: narK [H]

Synonym: PHZ_c0684

Alternate gene names: 197104150

Gene position: 742218-744965 (Clockwise)

Preceding gene: 197104143

Following gene: 197104151

Centisome position: 18.57

GC content: 70.31

Gene sequence:

>2748_bases
ATGGCGGTGACCCCAACGACGGAAGCGTCGCCCGGCGCAGGCCGGGCCCTTTGGGTCAGTACGGCCGCGTTCACGGTCTG
CTTCGCCGTCTGGACGATCTTCTCCATCATCGGCCTTGGGATGAAGGACGAACTCGGGCTCAGCGAGACCCAGTTCGGCC
TGCTCGTCGGCACCCCGATCCTGACCGGTTCGCTCAGCCGCCTCGTGCTCGGCATCTGGGCCGACCAGTACGGCGGGCGG
CGGGTGTTCGCCATCGTGATGGCCCTGGGGGCCGCCGCGACCTTCCTGCTGTCGTTCGCCGACACCTACCTCGAGCTGCT
GCTGGCGGCCCTGGGCGTGGGGATCGCGGGCGGGTCCTTCGCCGTGGGCGTCGCCTACGTCTCCAAGTTCTACCCGCAGG
AGCGGCAGGGGACGGCGCTGGGGATCTTCGGGGCCGGCAACGTGGGCTCGGCCGTCACCAAGTTCCTGGCGCCGTTCGTG
ATGGTCGCCATGGGCTGGCAGACGGTCGCCCAGCTCTGGGCCGCCGCCCTCCTGGTGATGGCGGTGATCTTCTGGTTCAC
GACCCGCGAGGACCCGGACCTCGCCCGCCGGCGGGCGGAAGGGGTCAAGCCGGCCTCCTTCGCCGAACAGCTGCGCCCCC
TGGCCAACGTCCAGGTCTGGCGCTTCTCGCTCTACTACTTCTTCGTCTTCGGCGCCTTCGTGGCCCTGGCGCTGTGGCTG
CCGCGCTACCTCGTCGGCGTCTACGGGCTCGACGTGAAGGCGGCGGGCATGCTGGCGGCCTTCTACTCCATCCCGGCCAG
CCTGTTCCGGATCTACGGCGGGCGCCTCTCGGACCGCTTCGGCGCGCGCAAGGTCCTCTACTGGACGTTCGGCGTCTCGG
CGCTGTGCACCTTCATGCTCTCGTACCCGCCGACCGACTACGTGATCCACGGCATCCAGGGCCCGATCGCGTTCTCGACC
TCGATGGGGCTGGTCCCGTTCGTCGTGACCATCTTCGTGCTGGGCTTCTTCATGAGCCTGGGCAAGGCGGCCGTGTTCCG
GCACATCCCGGTCTACTACCCGCAGCGCGTCGGAGCAGTGGGCGGGGTGGTCGGGATGGTCGGCGGCGTGGGCGGCTTCG
TCCTGCCGCTCGTCTTCGGCGTGCTCAACGACCTGACCGGCGTCTGGACGAGCTGCTTCTTCCTGCTCTTCCTCCTCGTT
TCCCTGGCGCTCGCCTGGATGCACTTCGCGGTGCGGCGCATGGAGGCGGCGGCGACCGCGCCGCGCGAGACCCTTCCCGA
GCTGCCGGAGATGGCGCCGATCCACGACGCCGCGCGCGTGGCGCCGGGGCCGCAGCCCGCGGGCAAGGTCGTCCCCGTCC
GGCGGCCGGCGATCGCCGACTGGCGGCCCGAGGACCCCGTGTTCTGGCAGGCGAGCGGCCGGCGCATCGCCCGCCGCAAC
CTGTGGATCTCCACCTACTGTCTGCTGCTCGCCTTCGCGGTCTGGATGGTCTGGAGCGTGGTCGTCGCGCGCCTGCCGGC
CGTCGGATTCCAGTTCACGCCCGACCAGCTGTTCTGGCTGGCGGCGCTGCCGGGGATCTCCGGCGCGGCGCTGCGGATCT
TCTACGCCTTCCTCGTGCCGGTGTTCGGCGGCCGGCTCTGGACGGCGATGTCGACGGCCTCGCTGCTGATCCCGGCGTTC
GGCATCGGCTACGCCGTGCAGGATCCTTCGACGCCGTACCTGATCTTCGTGGTCCTGGCGCTGCTGTGCGGCCTGGGCGG
CGGCAACTTCGCCTCGTCGATGGCCAACATCAGCTTCTTCTTCCCGAAGGCGGAGAAGGGCTCGGCCCTGGCCCTCAACG
CCGGCCTCGGCAACCTGGGCGTCAGCGTCATGCAGTTCGTGGTGCCGGTCGCCGTCACCATGAGCATCTTCGGCATCCTG
GGCGGCGAGCCGCAGACCACCGTCGAGGGCGACCGGCTGTGGATGCAGAACGCCGGCTTCCTGTGGGTTCCGTTCGTGAT
CGCGGGCGCAGTGCTCGCCTGGCTCGGCATGGACGACCTGGCCAGCGCCAAGTCCTCATTCGCCGAGCAGGCCAGCATCT
TCCAGCGCCGGCACACCTGGCTGATGTGCTGGCTCTACACCGGCACCTTCGGCTCGTTCATCGGCTTCTCGGCGGGCTTC
CCGCTGCTGGCGCGGCTCGCTTTCCCGGACGTGGATTCCCTGCGCTACGTGTTCCTCGGCCCGCTGGTCGGGGCGGCCTC
GCGCGCGGCCACCGGCTGGCTGGCCGACCGGTACGGCGGGGCGCGCATCACCTTCCTCGTGTTCCTCGGCATGATCGCGG
CCGTGGGGGGGATGCTCTGGTTCCTGAACGCCGGTTCGTTCTGGGGCTTCTTCGCCATGGTGATGCTGCTGTTCTTCTTC
AGCGGCGTCGGCAACGCCTCGACCTTCCAGATGATCCCGGCCATCTGGCGCGAGACGGTGGGACGCGCCGCCGGCGCCGC
CCAGGCCGACCGCGAGGCGGCGGCGGTGATTGGCTTCACCTCCGCCATCGCCGCCTTCGGCGCCTTCTTCATCCCCAAGG
CCTACGGCGCGTCCATCGCCGGGACCGGGGCGGCCGACGCGGCCCTGTGGGGCTTCCTCGCCTTCTACGCGGTCTGCGCC
GTCCTGACCTTCGCGATCTACTCCCGTCCCGGCGGCCTGCTGCACGCCGTCGAGCGCCGCCAAGGCGCAGCCCCCGCAGG
ACCCACAGCCCTGGAAGGAAGCCGATGA

Upstream 100 bases:

>100_bases
ACGCACAGTGTTACGCAAAGTCGTCCGCTGGCGACAGCCGGCGTTCGGGGAACCGCGGCGTGACTCGCGAGTTGCCCAAT
CGAGGGAAGGCGGGACGGGG

Downstream 100 bases:

>100_bases
GCCATCTCCTGGACCGCCTGACCTATTTCCGCAGAGAGAAGGAGACGTTCTCGGACGGGCACGGCGTGACGGTCCGGGAG
GACCGCGGCTGGGAGGACGC

Product: Nitrate transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 915; Mature: 914

Protein sequence:

>915_residues
MAVTPTTEASPGAGRALWVSTAAFTVCFAVWTIFSIIGLGMKDELGLSETQFGLLVGTPILTGSLSRLVLGIWADQYGGR
RVFAIVMALGAAATFLLSFADTYLELLLAALGVGIAGGSFAVGVAYVSKFYPQERQGTALGIFGAGNVGSAVTKFLAPFV
MVAMGWQTVAQLWAAALLVMAVIFWFTTREDPDLARRRAEGVKPASFAEQLRPLANVQVWRFSLYYFFVFGAFVALALWL
PRYLVGVYGLDVKAAGMLAAFYSIPASLFRIYGGRLSDRFGARKVLYWTFGVSALCTFMLSYPPTDYVIHGIQGPIAFST
SMGLVPFVVTIFVLGFFMSLGKAAVFRHIPVYYPQRVGAVGGVVGMVGGVGGFVLPLVFGVLNDLTGVWTSCFFLLFLLV
SLALAWMHFAVRRMEAAATAPRETLPELPEMAPIHDAARVAPGPQPAGKVVPVRRPAIADWRPEDPVFWQASGRRIARRN
LWISTYCLLLAFAVWMVWSVVVARLPAVGFQFTPDQLFWLAALPGISGAALRIFYAFLVPVFGGRLWTAMSTASLLIPAF
GIGYAVQDPSTPYLIFVVLALLCGLGGGNFASSMANISFFFPKAEKGSALALNAGLGNLGVSVMQFVVPVAVTMSIFGIL
GGEPQTTVEGDRLWMQNAGFLWVPFVIAGAVLAWLGMDDLASAKSSFAEQASIFQRRHTWLMCWLYTGTFGSFIGFSAGF
PLLARLAFPDVDSLRYVFLGPLVGAASRAATGWLADRYGGARITFLVFLGMIAAVGGMLWFLNAGSFWGFFAMVMLLFFF
SGVGNASTFQMIPAIWRETVGRAAGAAQADREAAAVIGFTSAIAAFGAFFIPKAYGASIAGTGAADAALWGFLAFYAVCA
VLTFAIYSRPGGLLHAVERRQGAAPAGPTALEGSR

Sequences:

>Translated_915_residues
MAVTPTTEASPGAGRALWVSTAAFTVCFAVWTIFSIIGLGMKDELGLSETQFGLLVGTPILTGSLSRLVLGIWADQYGGR
RVFAIVMALGAAATFLLSFADTYLELLLAALGVGIAGGSFAVGVAYVSKFYPQERQGTALGIFGAGNVGSAVTKFLAPFV
MVAMGWQTVAQLWAAALLVMAVIFWFTTREDPDLARRRAEGVKPASFAEQLRPLANVQVWRFSLYYFFVFGAFVALALWL
PRYLVGVYGLDVKAAGMLAAFYSIPASLFRIYGGRLSDRFGARKVLYWTFGVSALCTFMLSYPPTDYVIHGIQGPIAFST
SMGLVPFVVTIFVLGFFMSLGKAAVFRHIPVYYPQRVGAVGGVVGMVGGVGGFVLPLVFGVLNDLTGVWTSCFFLLFLLV
SLALAWMHFAVRRMEAAATAPRETLPELPEMAPIHDAARVAPGPQPAGKVVPVRRPAIADWRPEDPVFWQASGRRIARRN
LWISTYCLLLAFAVWMVWSVVVARLPAVGFQFTPDQLFWLAALPGISGAALRIFYAFLVPVFGGRLWTAMSTASLLIPAF
GIGYAVQDPSTPYLIFVVLALLCGLGGGNFASSMANISFFFPKAEKGSALALNAGLGNLGVSVMQFVVPVAVTMSIFGIL
GGEPQTTVEGDRLWMQNAGFLWVPFVIAGAVLAWLGMDDLASAKSSFAEQASIFQRRHTWLMCWLYTGTFGSFIGFSAGF
PLLARLAFPDVDSLRYVFLGPLVGAASRAATGWLADRYGGARITFLVFLGMIAAVGGMLWFLNAGSFWGFFAMVMLLFFF
SGVGNASTFQMIPAIWRETVGRAAGAAQADREAAAVIGFTSAIAAFGAFFIPKAYGASIAGTGAADAALWGFLAFYAVCA
VLTFAIYSRPGGLLHAVERRQGAAPAGPTALEGSR
>Mature_914_residues
AVTPTTEASPGAGRALWVSTAAFTVCFAVWTIFSIIGLGMKDELGLSETQFGLLVGTPILTGSLSRLVLGIWADQYGGRR
VFAIVMALGAAATFLLSFADTYLELLLAALGVGIAGGSFAVGVAYVSKFYPQERQGTALGIFGAGNVGSAVTKFLAPFVM
VAMGWQTVAQLWAAALLVMAVIFWFTTREDPDLARRRAEGVKPASFAEQLRPLANVQVWRFSLYYFFVFGAFVALALWLP
RYLVGVYGLDVKAAGMLAAFYSIPASLFRIYGGRLSDRFGARKVLYWTFGVSALCTFMLSYPPTDYVIHGIQGPIAFSTS
MGLVPFVVTIFVLGFFMSLGKAAVFRHIPVYYPQRVGAVGGVVGMVGGVGGFVLPLVFGVLNDLTGVWTSCFFLLFLLVS
LALAWMHFAVRRMEAAATAPRETLPELPEMAPIHDAARVAPGPQPAGKVVPVRRPAIADWRPEDPVFWQASGRRIARRNL
WISTYCLLLAFAVWMVWSVVVARLPAVGFQFTPDQLFWLAALPGISGAALRIFYAFLVPVFGGRLWTAMSTASLLIPAFG
IGYAVQDPSTPYLIFVVLALLCGLGGGNFASSMANISFFFPKAEKGSALALNAGLGNLGVSVMQFVVPVAVTMSIFGILG
GEPQTTVEGDRLWMQNAGFLWVPFVIAGAVLAWLGMDDLASAKSSFAEQASIFQRRHTWLMCWLYTGTFGSFIGFSAGFP
LLARLAFPDVDSLRYVFLGPLVGAASRAATGWLADRYGGARITFLVFLGMIAAVGGMLWFLNAGSFWGFFAMVMLLFFFS
GVGNASTFQMIPAIWRETVGRAAGAAQADREAAAVIGFTSAIAAFGAFFIPKAYGASIAGTGAADAALWGFLAFYAVCAV
LTFAIYSRPGGLLHAVERRQGAAPAGPTALEGSR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=446, Percent_Identity=53.3632286995516, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1787743, Length=451, Percent_Identity=51.219512195122, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI2367379, Length=247, Percent_Identity=26.7206477732794, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1786686, Length=368, Percent_Identity=23.6413043478261, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 97888; Mature: 97757

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTPTTEASPGAGRALWVSTAAFTVCFAVWTIFSIIGLGMKDELGLSETQFGLLVGTPI
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCHH
LTGSLSRLVLGIWADQYGGRRVFAIVMALGAAATFLLSFADTYLELLLAALGVGIAGGSF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AVGVAYVSKFYPQERQGTALGIFGAGNVGSAVTKFLAPFVMVAMGWQTVAQLWAAALLVM
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVIFWFTTREDPDLARRRAEGVKPASFAEQLRPLANVQVWRFSLYYFFVFGAFVALALWL
HHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
PRYLVGVYGLDVKAAGMLAAFYSIPASLFRIYGGRLSDRFGARKVLYWTFGVSALCTFML
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEHHHHHHHHHHHH
SYPPTDYVIHGIQGPIAFSTSMGLVPFVVTIFVLGFFMSLGKAAVFRHIPVYYPQRVGAV
CCCCHHHEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GGVVGMVGGVGGFVLPLVFGVLNDLTGVWTSCFFLLFLLVSLALAWMHFAVRRMEAAATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRETLPELPEMAPIHDAARVAPGPQPAGKVVPVRRPAIADWRPEDPVFWQASGRRIARRN
CHHHHCCCCCCCCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCCCHHHHH
LWISTYCLLLAFAVWMVWSVVVARLPAVGFQFTPDQLFWLAALPGISGAALRIFYAFLVP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHHHHH
VFGGRLWTAMSTASLLIPAFGIGYAVQDPSTPYLIFVVLALLCGLGGGNFASSMANISFF
HHCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEE
FPKAEKGSALALNAGLGNLGVSVMQFVVPVAVTMSIFGILGGEPQTTVEGDRLWMQNAGF
ECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
LWVPFVIAGAVLAWLGMDDLASAKSSFAEQASIFQRRHTWLMCWLYTGTFGSFIGFSAGF
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCHHHHHHHCCCH
PLLARLAFPDVDSLRYVFLGPLVGAASRAATGWLADRYGGARITFLVFLGMIAAVGGMLW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLNAGSFWGFFAMVMLLFFFSGVGNASTFQMIPAIWRETVGRAAGAAQADREAAAVIGFT
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
SAIAAFGAFFIPKAYGASIAGTGAADAALWGFLAFYAVCAVLTFAIYSRPGGLLHAVERR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QGAAPAGPTALEGSR
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVTPTTEASPGAGRALWVSTAAFTVCFAVWTIFSIIGLGMKDELGLSETQFGLLVGTPI
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEECCHH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CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]