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The map label for this gene is pyrB [H]

Identifier: 195952699

GI number: 195952699

Start: 280180

End: 281046

Strand: Direct

Name: pyrB [H]

Synonym: HY04AAS1_0321

Alternate gene names: 195952699

Gene position: 280180-281046 (Clockwise)

Preceding gene: 195952698

Following gene: 195952700

Centisome position: 17.97

GC content: 33.1

Gene sequence:

>867_bases
ATGAAATATATAATAGAAGCCCAAAACATAAAAGATACATACAAAACTATATTAAATAGGGCTAAAGAGTTTAAGGAAAA
AAGGATATATCCCACTTACAAAGCATCTTGTGCTCTTTTTTTTGAAGAACCATCCACAAGAACAAGGCTTTCTTTTGAAA
AAGCCGCCAAGAATTTGGGTTGTGAGACTTATTATATACAAGGTAATTTTTCATCGGCTGTAAAGGGTGAAAGTTTTTTT
GATACGCTTTTATCTTTTAAAAAGATTGACATAGATATCGTTGTTTTTCGTACATCTGATATCGTATTTGATTATGAGCC
ACTTCTAAATATAGATATAGGCCTTATAAATGCTGGAGATGGTACGCACGAGCATCCGTCTCAGGGTCTTCTTGATGTTT
TTACGCTTTTAGAAAAAGTAAATAGTTTGGAAAATCAAAATATACTATACGTAGGAGATGTTTACCACAGTAGAGTGTTT
AGGTCAGGAGCTTACTTTTTCAAGTTAGAAGGGGCAAATATAGGTGTTTGTGGATATCCAAGCTTTATACCAAAAGATTA
TCCTTTTATGGACAAAGCTTTTACAAATTTGGATGAGGCGATTGATTGGGCTCATCATGTGATAGCTCTACGCATTCAAA
AAGAAAGACACAAAGAAGCTTACAATTTAGAAAGTTATTTTGACATGTATGGTATAACAAAAGAAAGGTATAATAAGATA
AGAGGATTTTTGATGCATCCTGGCCCTGTAAATAGGGATGTTGATATAGATGGTGATTTGTTGTATAAAGATAAATCTTT
GATAGCAAATCAGATAGAAAACGGCGTGTTTGTCCGTATGGCTATGATAGAATATGTTTTAGGATAG

Upstream 100 bases:

>100_bases
AGCGCTTTTGCCACTTACGCTTTGAGACAGCTTGGTTTTGATGCTTTTAATATGATAGGTGGTATATTAAATTGGCCTTA
CGAAACAGAAAGAGGCTAAT

Downstream 100 bases:

>100_bases
GAGATGGTTTATGTATACATATATAAGATATATGGACACAAACAAGATAGGCCTTTTGGAAGGTGCAAATGAAGTTTTAC
CAAAAGACTCTTACGTAATC

Product: aspartate/ornithine carbamoyltransferase carbamoyl-P binding domain

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKYIIEAQNIKDTYKTILNRAKEFKEKRIYPTYKASCALFFEEPSTRTRLSFEKAAKNLGCETYYIQGNFSSAVKGESFF
DTLLSFKKIDIDIVVFRTSDIVFDYEPLLNIDIGLINAGDGTHEHPSQGLLDVFTLLEKVNSLENQNILYVGDVYHSRVF
RSGAYFFKLEGANIGVCGYPSFIPKDYPFMDKAFTNLDEAIDWAHHVIALRIQKERHKEAYNLESYFDMYGITKERYNKI
RGFLMHPGPVNRDVDIDGDLLYKDKSLIANQIENGVFVRMAMIEYVLG

Sequences:

>Translated_288_residues
MKYIIEAQNIKDTYKTILNRAKEFKEKRIYPTYKASCALFFEEPSTRTRLSFEKAAKNLGCETYYIQGNFSSAVKGESFF
DTLLSFKKIDIDIVVFRTSDIVFDYEPLLNIDIGLINAGDGTHEHPSQGLLDVFTLLEKVNSLENQNILYVGDVYHSRVF
RSGAYFFKLEGANIGVCGYPSFIPKDYPFMDKAFTNLDEAIDWAHHVIALRIQKERHKEAYNLESYFDMYGITKERYNKI
RGFLMHPGPVNRDVDIDGDLLYKDKSLIANQIENGVFVRMAMIEYVLG
>Mature_288_residues
MKYIIEAQNIKDTYKTILNRAKEFKEKRIYPTYKASCALFFEEPSTRTRLSFEKAAKNLGCETYYIQGNFSSAVKGESFF
DTLLSFKKIDIDIVVFRTSDIVFDYEPLLNIDIGLINAGDGTHEHPSQGLLDVFTLLEKVNSLENQNILYVGDVYHSRVF
RSGAYFFKLEGANIGVCGYPSFIPKDYPFMDKAFTNLDEAIDWAHHVIALRIQKERHKEAYNLESYFDMYGITKERYNKI
RGFLMHPGPVNRDVDIDGDLLYKDKSLIANQIENGVFVRMAMIEYVLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=29.5081967213115, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=30.1282051282051, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=276, Percent_Identity=29.7101449275362, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322331, Length=266, Percent_Identity=28.9473684210526, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642586, Length=272, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=9e-18,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33230; Mature: 33230

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIIEAQNIKDTYKTILNRAKEFKEKRIYPTYKASCALFFEEPSTRTRLSFEKAAKNLG
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
CETYYIQGNFSSAVKGESFFDTLLSFKKIDIDIVVFRTSDIVFDYEPLLNIDIGLINAGD
CEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCEEEECCCCEEEEEEEEECCC
GTHEHPSQGLLDVFTLLEKVNSLENQNILYVGDVYHSRVFRSGAYFFKLEGANIGVCGYP
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCEEEECCCC
SFIPKDYPFMDKAFTNLDEAIDWAHHVIALRIQKERHKEAYNLESYFDMYGITKERYNKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHCCHHHHHHHHCCCHHHHHHH
RGFLMHPGPVNRDVDIDGDLLYKDKSLIANQIENGVFVRMAMIEYVLG
HEEEECCCCCCCCCCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKYIIEAQNIKDTYKTILNRAKEFKEKRIYPTYKASCALFFEEPSTRTRLSFEKAAKNLG
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
CETYYIQGNFSSAVKGESFFDTLLSFKKIDIDIVVFRTSDIVFDYEPLLNIDIGLINAGD
CEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCEEEECCCCEEEEEEEEECCC
GTHEHPSQGLLDVFTLLEKVNSLENQNILYVGDVYHSRVFRSGAYFFKLEGANIGVCGYP
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCEEEEECCCEEEECCCC
SFIPKDYPFMDKAFTNLDEAIDWAHHVIALRIQKERHKEAYNLESYFDMYGITKERYNKI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHCCHHHHHHHHCCCHHHHHHH
RGFLMHPGPVNRDVDIDGDLLYKDKSLIANQIENGVFVRMAMIEYVLG
HEEEECCCCCCCCCCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]