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The map label for this gene is flgJ [H]

Identifier: 194738323

GI number: 194738323

Start: 1222758

End: 1223708

Strand: Direct

Name: flgJ [H]

Synonym: SeSA_A1250

Alternate gene names: 194738323

Gene position: 1222758-1223708 (Clockwise)

Preceding gene: 194737316

Following gene: 194736355

Centisome position: 25.97

GC content: 56.15

Gene sequence:

>951_bases
ATGATCGGAGACGGTAAATTGCTGGCCAGCGCGGCCTGGGATGCGCAATCTCTGAACGAACTGAAAGCGAAAGCGGGCCA
GGACCCGGCGGCGAATATCCGTCCTGTGGCCCGTCAGGTGGAAGGGATGTTTGTTCAGATGATGCTGAAAAGTATGCGCG
AGGCTTTACCCAAAGATGGTTTATTCAGCAGCGATCAGACCCGTCTGTATACCAGCATGTATGACCAGCAGATCGCCCAG
CAGATGACCGCCGGTAAGGGGTTGGGGCTGGCGGATATGATGGTTAAACAGATGACGGGCGGGCAGACGATGCCTGCAGA
TGATGCGCCGCAAGTACCGCTTAAATTCTCCCTGGAGACGGTAAACAGCTATCAAAATCAGGCGCTGACCCAACTGGTGC
GCAAAGCCATACCGAAAACGCCGGACAGCAGCGATGTGCCGCTCTCCGGCGACAGTAAAGACTTTCTGGCCCAGCTTTCG
CTCCCGGCGAGGCTGGCCAGCGAACAAAGCGGGGTGCCGCATCATCTGATTCTGGCGCAGGCGGCGCTGGAGTCGGGCTG
GGGGCAACGGCAAATCCTGCGGGAGAATGGCGAACCCAGCTATAACGTATTTGGCGTGAAAGCGACCGCCAGTTGGAAAG
GGCCGGTGACGGAAATCACCACCACTGAATACGAAAATGGCGAAGCGAAAAAAGTGAAAGCGAAATTCCGCGTCTATAGC
TCGTATCTGGAGGCGTTATCGGATTATGTCGCGCTGTTAACGCGTAACCCACGCTACGCTGCCGTGACCACTGCCGCCAC
GGCAGAGCAGGGCGCAGTGGCCCTGCAAAACGCCGGATACGCCACTGACCCGAATTACGCGCGTAAATTGACCAGCATGA
TTCAGCAGTTGAAAGCGATGAGTGAAAAGGTCAGCAAAACCTACAGCGCGAATCTCGACAATCTCTTTTAA

Upstream 100 bases:

>100_bases
GCGCGCTGAATGCGCTTGGCGCGACGCCGATGGACCTGATGTCGATTTTGCAGTCCATGCAGAGCGCGGGCTGTCTACGC
GCCAAACTGGAAATCATCTG

Downstream 100 bases:

>100_bases
ATTGCTCAAGTCCACGTAGTCGCTGCCGATAACAACGAGTATTGAAGGATTAAAAGGAACCATCATGTCCAGCTTGATTA
ATCACGCCATGAGCGGACTT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDVPLSGDSKDFLAQLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF

Sequences:

>Translated_316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDVPLSGDSKDFLAQLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF
>Mature_316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDVPLSGDSKDFLAQLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=316, Percent_Identity=83.5443037974684, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34355; Mature: 34355

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG
CCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLET
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHH
VNSYQNQALTQLVRKAIPKTPDSSDVPLSGDSKDFLAQLSLPARLASEQSGVPHHLILAQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHH
AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
HHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
SEKVSKTYSANLDNLF
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG
CCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLET
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHH
VNSYQNQALTQLVRKAIPKTPDSSDVPLSGDSKDFLAQLSLPARLASEQSGVPHHLILAQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHH
AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
HHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
SEKVSKTYSANLDNLF
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]