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The map label for this gene is rpoS [H]

Identifier: 194738113

GI number: 194738113

Start: 2970832

End: 2971824

Strand: Reverse

Name: rpoS [H]

Synonym: SeSA_A3074

Alternate gene names: 194738113

Gene position: 2971824-2970832 (Counterclockwise)

Preceding gene: 194737503

Following gene: 194734084

Centisome position: 63.11

GC content: 52.17

Gene sequence:

>993_bases
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGACGCGGAATTTGATGAGAACGGAGTAGAGGCTTTTGACGA
AAAAGCCTTGAGTGAAGAGGAACCCAGTGATAACGACCTGGCTGAAGAAGAGCTGTTATCGCAAGGGGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGGTATTCACCACTGTTAACAGCCGAAGAAGAAGTCTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCTTCTCGCCGTCGCATGATTGAGAGTAACCTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGACTGGCGTTGCTGGACCTGATTGAAGAGGGCAACCTGGGGCTTATCCGTGCAGTCGAGAAGT
TTGACCCGGAACGCGGGTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACAATCGAACGGGCGATTATGAAC
CAAACCCGTACGATTCGCTTGCCGATTCACATTGTTAAAGAGCTGAACGTATACCTGCGCACCGCACGTGAGTTGTCGCA
TAAACTGGACCACGAACCGAGTGCGGAAGAAATTGCAGAGCAACTGGATAAACCGGTTGATGACGTCAGCCGTATGCTTC
GTCTCAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGCGGTGATTCCGAAAAAGCGTTGCTGGACATTCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGACACCACGCAAGATGACGATATGAAACAGAGCATCGTCAAATGGTTGTTCGAACTGAA
CGCCAAACAGCGTGAAGTGCTGGCGCGCCGTTTCGGTCTGCTGGGATATGAAGCTGCGACACTGGAAGATGTAGGTCGTG
AAATCGGTCTTACGCGTGAACGTGTTCGTCAGATTCAGGTTGAAGGCCTGCGCCGTCTGCGCGAAATTCTGCAGACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Upstream 100 bases:

>100_bases
AATCCGTAAACCCGCTGCGTTATTTACCGCAGCGATAAAGCGGCGGAACCAGGCTTTGACTTGCTAGTTCCGTCAAGGGA
TCACGGGTAGGAGCCACCTT

Downstream 100 bases:

>100_bases
GTACCCTTGTCAAAAAAAGGCCAGTCGACAGACTGGCCTTTTTTTTTACCGTTTGTCTTCTGCCAGCAACGGCGGGATAC
TCGGCACCATTGGCGCGTTA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Sequences:

>Translated_330_residues
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE
>Mature_329_residues
SQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFAR
RALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQ
TRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADE
KENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQG
LNIEALFRE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=330, Percent_Identity=99.0909090909091, Blast_Score=653, Evalue=0.0,
Organism=Escherichia coli, GI1789448, Length=247, Percent_Identity=42.1052631578947, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37933; Mature: 37802

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLR
HCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
TARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
RVRQIQVEGLRRLREILQTQGLNIEALFRE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
SQNTLKVHDLNEDAEFDENGVEAFDEKALSEEEPSDNDLAEEELLSQGATQRVLDATQL
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
YLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLR
HCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
TARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
HHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
RVRQIQVEGLRRLREILQTQGLNIEALFRE
HHHHHHHHHHHHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]