Click here to switch to the map view.

The map label for this gene is pgaB [H]

Identifier: 194737619

GI number: 194737619

Start: 347917

End: 348738

Strand: Direct

Name: pgaB [H]

Synonym: SeSA_A0340

Alternate gene names: 194737619

Gene position: 347917-348738 (Clockwise)

Preceding gene: 194736030

Following gene: 194736448

Centisome position: 7.39

GC content: 51.58

Gene sequence:

>822_bases
ATGTCTTATGCACGACATTTACTGGTATTAATGTATCACCATGTCAGTGATAAACCCGGACAGATAACCTTATCTCCCCG
TACGTTCCGGGCGCAGATGAAATGGCTGGCTGAATCTGGCTGGAAAACCGTTACCGCAGCAGAGGTGGAAGCTTTTTATC
ATGGTGCAAGATTGCCTCGTAAAAGCGTCATGCTGACCTTTGATGGCGGCTGGCTGGATAACTGGTTGCAGGTTTTTCCG
GTGCTGCAGGAGTTTAATCTGCATGCGCATCTCTTTCTTGTGACCAGTTTGATCAGTGACGGACCGGTCCGTATTCCTGC
AGGCGAACCGGTGTACTCTCATGATGAGTGTCAGATGCTGGTTAAACAAGGCCGGGCTGATGAGGTCATGCTGCGCTGGT
CAGAGGTCCGGGAGATGCACCTCAGTGGCCTTGTTGAGTTTCACTCGCACACGCACACCCACCGACGCTGGGACCAGAAA
CCTGTGTCCCGTAATCCGTCGGATTTGCTTCGTGTCGATATTCTTCTTAGTCGTAAGCGGATGAGGGAGATGCTGGGTTA
CTGCAGTCAGCATCTGTGCTGGCCTGAGGGCTGGTATTGTTCTGACTATATTCATGTGGCTGAAGAGCTGGGGTTCACAT
ACCTGTATACCACAGAAAGGCGTATGAACAATCCAGTCATCGGTTCACAGCGTATTGGTCGTATCAACACAAAGGAGCGA
AAGAATGTGGGCTGGCTGAAACGTCGTCTGTTTTATCACACCACGCCCGGATTTTCTTCGCTGCTGGCCCGGCATAAGGG
GGCACGTCGGATAGCTGACTGA

Upstream 100 bases:

>100_bases
GGTATTCATATGTGATATTTAAGATTTTATGTTTAGGGGGCTTTTTCTTGTCCCCTCAGGCGTAAAAATAATTTATTTTT
TACATAAAGGAATAAAGCAT

Downstream 100 bases:

>100_bases
GCCGGAGACCAGGATGAAAGTGCTGCATACCGAATCCTCCCCCATCATCGGCGGGCAGGGGTTGTAGGCTATATCCCAAA
TGATGGTGCTGATTTTGAGC

Product: polysaccharide deacetylase family protein

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSYARHLLVLMYHHVSDKPGQITLSPRTFRAQMKWLAESGWKTVTAAEVEAFYHGARLPRKSVMLTFDGGWLDNWLQVFP
VLQEFNLHAHLFLVTSLISDGPVRIPAGEPVYSHDECQMLVKQGRADEVMLRWSEVREMHLSGLVEFHSHTHTHRRWDQK
PVSRNPSDLLRVDILLSRKRMREMLGYCSQHLCWPEGWYCSDYIHVAEELGFTYLYTTERRMNNPVIGSQRIGRINTKER
KNVGWLKRRLFYHTTPGFSSLLARHKGARRIAD

Sequences:

>Translated_273_residues
MSYARHLLVLMYHHVSDKPGQITLSPRTFRAQMKWLAESGWKTVTAAEVEAFYHGARLPRKSVMLTFDGGWLDNWLQVFP
VLQEFNLHAHLFLVTSLISDGPVRIPAGEPVYSHDECQMLVKQGRADEVMLRWSEVREMHLSGLVEFHSHTHTHRRWDQK
PVSRNPSDLLRVDILLSRKRMREMLGYCSQHLCWPEGWYCSDYIHVAEELGFTYLYTTERRMNNPVIGSQRIGRINTKER
KNVGWLKRRLFYHTTPGFSSLLARHKGARRIAD
>Mature_272_residues
SYARHLLVLMYHHVSDKPGQITLSPRTFRAQMKWLAESGWKTVTAAEVEAFYHGARLPRKSVMLTFDGGWLDNWLQVFPV
LQEFNLHAHLFLVTSLISDGPVRIPAGEPVYSHDECQMLVKQGRADEVMLRWSEVREMHLSGLVEFHSHTHTHRRWDQKP
VSRNPSDLLRVDILLSRKRMREMLGYCSQHLCWPEGWYCSDYIHVAEELGFTYLYTTERRMNNPVIGSQRIGRINTKERK
NVGWLKRRLFYHTTPGFSSLLARHKGARRIAD

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=247, Percent_Identity=28.3400809716599, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786322, Length=232, Percent_Identity=28.0172413793103, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 31968; Mature: 31837

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYARHLLVLMYHHVSDKPGQITLSPRTFRAQMKWLAESGWKTVTAAEVEAFYHGARLPR
CCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCC
KSVMLTFDGGWLDNWLQVFPVLQEFNLHAHLFLVTSLISDGPVRIPAGEPVYSHDECQML
CEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHH
VKQGRADEVMLRWSEVREMHLSGLVEFHSHTHTHRRWDQKPVSRNPSDLLRVDILLSRKR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MREMLGYCSQHLCWPEGWYCSDYIHVAEELGFTYLYTTERRMNNPVIGSQRIGRINTKER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHH
KNVGWLKRRLFYHTTPGFSSLLARHKGARRIAD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYARHLLVLMYHHVSDKPGQITLSPRTFRAQMKWLAESGWKTVTAAEVEAFYHGARLPR
CHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCC
KSVMLTFDGGWLDNWLQVFPVLQEFNLHAHLFLVTSLISDGPVRIPAGEPVYSHDECQML
CEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHH
VKQGRADEVMLRWSEVREMHLSGLVEFHSHTHTHRRWDQKPVSRNPSDLLRVDILLSRKR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MREMLGYCSQHLCWPEGWYCSDYIHVAEELGFTYLYTTERRMNNPVIGSQRIGRINTKER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCCCHHH
KNVGWLKRRLFYHTTPGFSSLLARHKGARRIAD
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]