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The map label for this gene is mlaE [H]

Identifier: 194737604

GI number: 194737604

Start: 3377288

End: 3378070

Strand: Reverse

Name: mlaE [H]

Synonym: SeSA_A3504

Alternate gene names: 194737604

Gene position: 3378070-3377288 (Counterclockwise)

Preceding gene: 194738105

Following gene: 194737260

Centisome position: 71.74

GC content: 57.73

Gene sequence:

>783_bases
ATGCTGTTAAATGCGCTGGCAGCACTCGGACACAGCGGGATCAAAACCGTCAGGACGTTCGGGCGCGCCGGGTTAATGTT
ATTCAATGCGATTATCGGTAAGCCGGAGTTTCGCAAACATGCGCCGCTGCTGGTGCGCCAGCTTTATAATGTGGGCGTCT
TGTCGATGCTTATCATTATTGTCTCCGGCGTATTCATCGGTATGGTGCTGGGGCTGCAAGGCTATCTGGTTCTGACTACT
TACAGCGCGGAGACCAGTCTTGGCATGTTAGTGGCGCTTTCGCTGCTGCGCGAGCTGGGGCCGGTGGTCGCGGCGTTGCT
GTTTGCCGGGCGGGCCGGGTCGGCGTTGACGGCGGAAATCGGCCTGATGCGGGCGACGGAGCAGCTCTCCAGTATGGAGA
TGATGGCGGTCGATCCATTGCGCCGGGTGATTTCGCCGCGCTTTTGGGCGGGCGTGATTTCGTTGCCGCTACTGACCATT
ATTTTTGTGGCGGTCGGTATCTGGGGCGGCTCGCTGGTCGGCGTAAGCTGGAAGGGAATCGACGCCGGGTTCTTCTGGTC
CGCGATGCAGAATGCCGTCGACTGGCGTATGGACCTGGTGAACTGTCTGATAAAGAGCGTGGTGTTCGCCATTACGGTAA
CATGGATTGCGTTATTCAACGGTTATGACGCAATACCGACTTCTGCCGGGATTAGCCGGGCAACGACGCGTACCGTGGTT
CACGCATCGTTGGCCGTTCTGGGGCTGGATTTTGTGCTGACCGCACTGATGTTTGGGAATTGA

Upstream 100 bases:

>100_bases
CGTCAGTTCCTTGACGGCATTGCCGACGGGCCGGTTCCGTTCCGCTATCCGGCGGGCGACTATCACCTTGATTTACTCGA
AACAGGGAGTTAAGCCACTC

Downstream 100 bases:

>100_bases
GTTCATGCAAACGAAAAAAAATGAAATTTGGGTAGGCGTTTTTTTGCTGGTAGCGTTGCTGGCGGCGCTGTTTGTTTGCC
TGAAGGCGGCAAATGTGACC

Product: toluene ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLNALAALGHSGIKTVRTFGRAGLMLFNAIIGKPEFRKHAPLLVRQLYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN

Sequences:

>Translated_260_residues
MLLNALAALGHSGIKTVRTFGRAGLMLFNAIIGKPEFRKHAPLLVRQLYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN
>Mature_260_residues
MLLNALAALGHSGIKTVRTFGRAGLMLFNAIIGKPEFRKHAPLLVRQLYNVGVLSMLIIIVSGVFIGMVLGLQGYLVLTT
YSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTI
IFVAVGIWGGSLVGVSWKGIDAGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
HASLAVLGLDFVLTALMFGN

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=260, Percent_Identity=96.1538461538462, Blast_Score=501, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27787; Mature: 27787

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNALAALGHSGIKTVRTFGRAGLMLFNAIIGKPEFRKHAPLLVRQLYNVGVLSMLIII
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTIIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DAGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HASLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLNALAALGHSGIKTVRTFGRAGLMLFNAIIGKPEFRKHAPLLVRQLYNVGVLSMLIII
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAEI
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTIIFVAVGIWGGSLVGVSWKGI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DAGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWIALFNGYDAIPTSAGISRATTRTVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HASLAVLGLDFVLTALMFGN
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]