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The map label for this gene is yafJ [H]

Identifier: 194737580

GI number: 194737580

Start: 366270

End: 367037

Strand: Direct

Name: yafJ [H]

Synonym: SeSA_A0360

Alternate gene names: 194737580

Gene position: 366270-367037 (Clockwise)

Preceding gene: 194736690

Following gene: 194735550

Centisome position: 7.78

GC content: 51.69

Gene sequence:

>768_bases
ATGTGCGAACTGCTTGGGATGAGCGCCAATGTGCCGACGGATATCTGCTTTAGCTTCACCGGACTGGTGCAGCGTGGCGG
CGGAACCGGGCCGCATAAAGACGGCTGGGGAATTACCTTTTACGAAGGAAAGGGCTGTCGCACGTTTAAAGATCCGCAAC
CGAGCTACCATTCGCCGATTGCGAAACTGGTACAGAATTACCCGATCAAGTCCTGCTCGGTTATCGCCCATATCCGTCAG
GCTAATCGCGGTGAAGTGGCGCTGGAAAATACACACCCTTTTACCCGGGAATTATGGGGACGCAACTGGACTTATGCTCA
TAACGGTCAGCTCAACGGTTATAAGTCGCTGGAAACCGGTAATTTTCGTCCGGTGGGGGAAACCGACAGCGAAAAAGCGT
TTTGCTGGCTATTGCATAAATTAACGCAGCGTTATCCCCGCACGCCGGGCAACATGACAGCGGTATTTAAATATATTGCG
ACACTGGCGACGGTATTACGTGAAAAAGGCGTTTTTAACATGCTGCTGTCAGATGGGCGATATGTGATGGCGTTTTGCTC
AACCCATTTGCACTGGATCACTCGCCGGGCGCCGTTCGGCGTAGCCACGTTGGTGGATCAGGACATGGAAATTGATTTCA
GTTCACAGACCACGCCAAATGACGTGGTTACCGTGATTGCTACCCAGCCGCTAACGGGGAATGAAACCTGGCAAAAGATT
ATGCCAGGCGAATGGGCGTTATTTTGCCTCGGAGAGCGTATAATTTGA

Upstream 100 bases:

>100_bases
AATTATTTAGGGTATAGCATATTGTTTTCTTTGATATTTTTAGTTTTGATTGGCTGTAAAAATTACGCCTTCCCGCTTAA
GGCGTTTGTGGAGGTGATGT

Downstream 100 bases:

>100_bases
GGCCAGCTGTGGCTGAACGACTTCGTGACTCAACGGTTTGCTCACGACGTAACGTCCATCAACGATAGAAACAGTTGGCG
GCTTATGCGTCTGTTCAAAA

Product: glutamine amidotransferase, class II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII

Sequences:

>Translated_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII
>Mature_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPIAKLVQNYPIKSCSVIAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIA
TLATVLREKGVFNMLLSDGRYVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWALFCLGERII

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=93.3333333333333, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28670; Mature: 28670

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
AKLVQNYPIKSCSVIAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIATLATVLREKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHH
MPGEWALFCLGERII
CCCCEEEEECCCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSYHSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
AKLVQNYPIKSCSVIAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLNGYKSLETG
HHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMTAVFKYIATLATVLREKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
YVMAFCSTHLHWITRRAPFGVATLVDQDMEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEHHHHHHHHHHHHCCCCCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCCCCHHHHHH
MPGEWALFCLGERII
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]