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The map label for this gene is intS [H]

Identifier: 194737537

GI number: 194737537

Start: 379241

End: 380431

Strand: Direct

Name: intS [H]

Synonym: SeSA_A0374

Alternate gene names: 194737537

Gene position: 379241-380431 (Clockwise)

Preceding gene: 194736743

Following gene: 194736902

Centisome position: 8.05

GC content: 46.52

Gene sequence:

>1191_bases
ATGATGCTGAATGCCCGTAAGGTTGAAGCCGCCAAAGGAAAAGAGAAGAGCTATAAGCTCGCTGATGGCGGCGGCCTGTA
TCTTCAGGTAGAACCCAATGGTTCACGATATTGGCGAATGAAGTATCGCTTCGCGGGTAAAGAAAAACGCCTATCTTTTG
GCGTCTATCCAGCTGTCAGCCTTGCTGATGCTAGGCAAAAGCGCGAAGACGCAAAGAAATTACTCGCAGCAGGTAACGAT
CCTGGCGAAGTAAAGAAAGCGAAGAAGCTGGCTTTGACCTCTGCCACTGAGATAGTCAACCCGTTCAAAGAGGTCGCGTT
AGAGTGGCACAAAATGAAGTCACCAAAATGGTCAAAGGGCTACGCTTCCGACATCCTTGAGGCCTTCGAGAAGGATATCT
TTCCCCATATTGGGCAACGACCTATTGCGGATATTAAACCCCTCGAACTACTAGAAGTGCTGAGACTGATAGAAGCCAGA
GGTGCGATGGAAAAAGCGAAGAAAGTCCGCCAGCGATGTGGAGAAGTTTTCCGTTATGCCATCGTAACAGGGCGAGCAAT
TTACAATCCTGCACCGGATCTCGCCAGCGCAATGCAGGGCCATGAAGCCGTCCATTATCCTTTCCTTAAAGCCACCGAAT
TACCCGAGTTCTTTACCGCCCTGAATGCCTATACAGGAAGCCCGATTGTTGTGCTGGGAGCACATTTACTTATCCTGACA
GGACTGAGAACGGGTGAGCTACGCGCCGCTGAATGGTGTGAAATTGATTTTGATAACGCTCTTTGGGAAATACCAAAAGA
ACGCATGAAAATGCGTCGTGCGCATCTTGTGCCGCTTTCAAATCAAGCATTAGCCCTTCTTGAAACCTTAAAAGAACTGA
CTGGAAACTATCCTTTGATGTTTCCTGGGCGTAACGATCCTAGCAAATCTATGAGTGAAGCAAGCATTAATCAGGTGTTT
AAACGTATTGGATATGCAGGGCGTGTTACGGGGCATGGTTTCAGGCATACGATGAGTACGATTCTACATGAGAAAGGATT
TAACAGCGCTTGGATTGAAACCCAGCTTGCTCATTTGGACAAAAATTCTATTCGTGGAATTTATAACCATGCCCAATATC
TCGAAGGGCGAAGAGAAATGATGCAATGGTATGCTAATTATTTATCTGAGCTGGTTGGTAATCATAAATGA

Upstream 100 bases:

>100_bases
TTTTCAGTCGTTTGAGGTCAACCCTTGTCTCTTGAAATCAACATGCTATTGGGGGCACAATCAGGGGCATGTTCTGTTCG
GTCCAGGAAATGTGCCCCCA

Downstream 100 bases:

>100_bases
ATGTTTTAAAAATTAATAATACTGATTCTTTGCTCAGAGACTACTACCAATTAGATGAAGCTTCAGAAAAAATTGGTTGT
AATGAAAAGGATATACTCTG

Product: site-specific recombinase, phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMLNARKVEAAKGKEKSYKLADGGGLYLQVEPNGSRYWRMKYRFAGKEKRLSFGVYPAVSLADARQKREDAKKLLAAGND
PGEVKKAKKLALTSATEIVNPFKEVALEWHKMKSPKWSKGYASDILEAFEKDIFPHIGQRPIADIKPLELLEVLRLIEAR
GAMEKAKKVRQRCGEVFRYAIVTGRAIYNPAPDLASAMQGHEAVHYPFLKATELPEFFTALNAYTGSPIVVLGAHLLILT
GLRTGELRAAEWCEIDFDNALWEIPKERMKMRRAHLVPLSNQALALLETLKELTGNYPLMFPGRNDPSKSMSEASINQVF
KRIGYAGRVTGHGFRHTMSTILHEKGFNSAWIETQLAHLDKNSIRGIYNHAQYLEGRREMMQWYANYLSELVGNHK

Sequences:

>Translated_396_residues
MMLNARKVEAAKGKEKSYKLADGGGLYLQVEPNGSRYWRMKYRFAGKEKRLSFGVYPAVSLADARQKREDAKKLLAAGND
PGEVKKAKKLALTSATEIVNPFKEVALEWHKMKSPKWSKGYASDILEAFEKDIFPHIGQRPIADIKPLELLEVLRLIEAR
GAMEKAKKVRQRCGEVFRYAIVTGRAIYNPAPDLASAMQGHEAVHYPFLKATELPEFFTALNAYTGSPIVVLGAHLLILT
GLRTGELRAAEWCEIDFDNALWEIPKERMKMRRAHLVPLSNQALALLETLKELTGNYPLMFPGRNDPSKSMSEASINQVF
KRIGYAGRVTGHGFRHTMSTILHEKGFNSAWIETQLAHLDKNSIRGIYNHAQYLEGRREMMQWYANYLSELVGNHK
>Mature_396_residues
MMLNARKVEAAKGKEKSYKLADGGGLYLQVEPNGSRYWRMKYRFAGKEKRLSFGVYPAVSLADARQKREDAKKLLAAGND
PGEVKKAKKLALTSATEIVNPFKEVALEWHKMKSPKWSKGYASDILEAFEKDIFPHIGQRPIADIKPLELLEVLRLIEAR
GAMEKAKKVRQRCGEVFRYAIVTGRAIYNPAPDLASAMQGHEAVHYPFLKATELPEFFTALNAYTGSPIVVLGAHLLILT
GLRTGELRAAEWCEIDFDNALWEIPKERMKMRRAHLVPLSNQALALLETLKELTGNYPLMFPGRNDPSKSMSEASINQVF
KRIGYAGRVTGHGFRHTMSTILHEKGFNSAWIETQLAHLDKNSIRGIYNHAQYLEGRREMMQWYANYLSELVGNHK

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=392, Percent_Identity=47.9591836734694, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI145693166, Length=396, Percent_Identity=41.1616161616162, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788974, Length=388, Percent_Identity=38.4020618556701, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44805; Mature: 44805

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLNARKVEAAKGKEKSYKLADGGGLYLQVEPNGSRYWRMKYRFAGKEKRLSFGVYPAVS
CCCCCCHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEEEEECCCCCCCEECCCCHHH
LADARQKREDAKKLLAAGNDPGEVKKAKKLALTSATEIVNPFKEVALEWHKMKSPKWSKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YASDILEAFEKDIFPHIGQRPIADIKPLELLEVLRLIEARGAMEKAKKVRQRCGEVFRYA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IVTGRAIYNPAPDLASAMQGHEAVHYPFLKATELPEFFTALNAYTGSPIVVLGAHLLILT
HHHCCHHCCCCHHHHHHHCCCCCHHCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GLRTGELRAAEWCEIDFDNALWEIPKERMKMRRAHLVPLSNQALALLETLKELTGNYPLM
CCCCCCCCCCHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
FPGRNDPSKSMSEASINQVFKRIGYAGRVTGHGFRHTMSTILHEKGFNSAWIETQLAHLD
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCC
KNSIRGIYNHAQYLEGRREMMQWYANYLSELVGNHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMLNARKVEAAKGKEKSYKLADGGGLYLQVEPNGSRYWRMKYRFAGKEKRLSFGVYPAVS
CCCCCCHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEEEEECCCCCCCEECCCCHHH
LADARQKREDAKKLLAAGNDPGEVKKAKKLALTSATEIVNPFKEVALEWHKMKSPKWSKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YASDILEAFEKDIFPHIGQRPIADIKPLELLEVLRLIEARGAMEKAKKVRQRCGEVFRYA
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IVTGRAIYNPAPDLASAMQGHEAVHYPFLKATELPEFFTALNAYTGSPIVVLGAHLLILT
HHHCCHHCCCCHHHHHHHCCCCCHHCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GLRTGELRAAEWCEIDFDNALWEIPKERMKMRRAHLVPLSNQALALLETLKELTGNYPLM
CCCCCCCCCCHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
FPGRNDPSKSMSEASINQVFKRIGYAGRVTGHGFRHTMSTILHEKGFNSAWIETQLAHLD
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCC
KNSIRGIYNHAQYLEGRREMMQWYANYLSELVGNHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]