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The map label for this gene is bcr [H]

Identifier: 194737450

GI number: 194737450

Start: 2342949

End: 2344139

Strand: Reverse

Name: bcr [H]

Synonym: SeSA_A2461

Alternate gene names: 194737450

Gene position: 2344139-2342949 (Counterclockwise)

Preceding gene: 194737206

Following gene: 194738183

Centisome position: 49.78

GC content: 53.99

Gene sequence:

>1191_bases
GTGACCACCCGGCAGCACTCTTCCTTTGCCATTGTCTTTATTCTTGGCCTGTTGGCCATGTTAATGCCGCTGTCGATTGA
TATGTATCTTCCAGCGCTGCCGGTGATTTCTGCGCAATTCGGCGTGCCTGCCGGTAGCGCGCAGATGACGCTCAGCACCT
ATATTCTGGGGTTTGCGCTGGGTCAGCTTATCTATGGACCGATGGCGGATAGCCTCGGGCGTAAGCCGGTCATCCTGGGC
GGGACGCTGGTATTTGCCGCGGCGGCGGTTGCCTGTGCGTTGGCGCAGACTATCGATCAACTGATCGTGATGCGTTTCTT
TCACGGTTTGGCGGCCGCAGCGGCAAGCGTCGTCATCAATGCGCTGATGCGGGATATTTATCCAAAGGAAGAGTTTTCGC
GCATGATGTCATTTGTCATGCTGGTCACTACGATAGCGCCATTAATGGCGCCCATTGTCGGCGGCTGGGTGTTGGTATGG
TTAAGCTGGCACTATATCTTCTGGATACTGGCCATTGCGGCGATTCTGGCGTCAGTCATGATCTTTGCTCTGATTAAAGA
GACGCTGCCCGTTGAGCGGCGTCAGCCTTTTCATATTCGTACCACAATAGGTAACTTTGCCGCGTTGTTTCGCCACAAAC
GCGTACTGAGCTATATGCTGGCGAGCGGGTTTAGTTTTGCCGGTATGTTCTCTTTTTTGAGCGCGGGGCCGTTTGTTTAT
ATCGAAATTAATCATGTTCCGCCACAGGATTTCGGCTACTACTTCGCGTTGAACATCGTATTTCTGTTTGTGATGACGAT
TATCAACAGCCGTTTTGTCAGACGGGTAGGGGCGCTAAACATGTTTCGGGCCGGGCTATGGATTCAGTTTGCGATGGCGG
TGTGGATGGTTTTCAGCGCGCTGATGGGCATTGGATTCTGGGCGCTGGTGGTCGGCGTTGCGGCGTTTGTCGGCTGTGTG
TCGATGGTTTCGTCCAATGCGATGGCGGTCATTTTGGATGAGTTTCCGCATATGGCCGGAACGGCGTCTTCGTTGGCGGG
CACTTTCCGCTTTGGTATTGGCGCTATCGTCGGCGCGTTGCTGTCGCTGGCTACCTTTACCAGTGCGTGGCCGATGATCT
GGTCGATTGCGCTTTGCGCCGCCTGTTCCATTCTGTTTTATCTCTACGCCAGCCGTCCCAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TACGCTGGATGAGAATCTGGCTCCCGGCGAGTACCGCCCGTTGACTGAAGAAGAAATCGCCAGCGTCGGCTAACTATCTC
GTTAAATTCAGGAGTTCGAT

Downstream 100 bases:

>100_bases
CGGGCGCGGTGTCCCTCAATGGCGCCGAAAAATGCGCAGGCGGCGTTGGGGGGCGCTATCCGCTAGACGATGGCAAAGCC
AAACGGCTTTCATAGTTGAT

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSAQMTLSTYILGFALGQLIYGPMADSLGRKPVILG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILAIAAILASVMIFALIKETLPVERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVPPQDFGYYFALNIVFLFVMTIINSRFVRRVGALNMFRAGLWIQFAMAVWMVFSALMGIGFWALVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFTSAWPMIWSIALCAACSILFYLYASRPKKR

Sequences:

>Translated_396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSAQMTLSTYILGFALGQLIYGPMADSLGRKPVILG
GTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILAIAAILASVMIFALIKETLPVERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVPPQDFGYYFALNIVFLFVMTIINSRFVRRVGALNMFRAGLWIQFAMAVWMVFSALMGIGFWALVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFTSAWPMIWSIALCAACSILFYLYASRPKKR
>Mature_395_residues
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSAQMTLSTYILGFALGQLIYGPMADSLGRKPVILGG
TLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWL
SWHYIFWILAIAAILASVMIFALIKETLPVERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYI
EINHVPPQDFGYYFALNIVFLFVMTIINSRFVRRVGALNMFRAGLWIQFAMAVWMVFSALMGIGFWALVVGVAAFVGCVS
MVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFTSAWPMIWSIALCAACSILFYLYASRPKKR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=93.6868686868687, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=358, Percent_Identity=29.608938547486, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790146, Length=241, Percent_Identity=29.4605809128631, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87082312, Length=337, Percent_Identity=24.9258160237389, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790794, Length=252, Percent_Identity=22.6190476190476, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787065, Length=252, Percent_Identity=24.2063492063492, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081738, Length=362, Percent_Identity=26.2430939226519, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=27.810650887574, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=162, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=205, Percent_Identity=23.4146341463415, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325414, Length=202, Percent_Identity=24.7524752475248, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=202, Percent_Identity=24.7524752475248, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=182, Percent_Identity=23.0769230769231, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43255; Mature: 43123

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSAQMTLSTYILGFAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
GQLIYGPMADSLGRKPVILGGTLVFAAAAVACALAQTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILAIAAILASVM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFALIKETLPVERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVPPQDFGYYFALNIVFLFVMTIINSRFVRRVGALNMFRAGLWIQFAMAVWMVFSA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMGIGFWALVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFTSAWPMIWSIALCAACSILFYLYASRPKKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]