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The map label for this gene is yneJ [H]

Identifier: 194737349

GI number: 194737349

Start: 2068560

End: 2069438

Strand: Direct

Name: yneJ [H]

Synonym: SeSA_A2178

Alternate gene names: 194737349

Gene position: 2068560-2069438 (Clockwise)

Preceding gene: 194736187

Following gene: 194736143

Centisome position: 43.93

GC content: 43.91

Gene sequence:

>879_bases
ATGGATCTTAACCAGCTTAAAACATTTGTTACCGTTGCGGAAACCGCCAGCCTGACTCGCGCAGCAGGACTTCTCTACCT
AAGTCAACCAGCGGTAAGCGCCCATATAAAGCAACTTGAAACCGAGTTCAATGTAAAGCTATTCAAACGCACCCCCAGAG
GCATGGAGCTGACCTCTACAGGTCTCATCATGAGAGATGAAGCACGTCTTGCACTTGATGCCGCAAACAAATTTGTAAAT
AAAGCCCGTACACTTCATAATATAGAGAACTGTGCCTTAGGCACTATTTCAGTTCCAGTAATCCTTAATCTACCATTAGT
ACTATCACAACTACGGCTGCGGCACCAGAATGTCAGTTTGACGATCCAGCAGAATATTTCGGGGTATATTATTGAAGACA
TTAAGAACGGTAAGCTCGATGCTGGCTTTGTCATTGGGGAAGTTAATGACTCTCAGTTAGGGATTATTCCTGTTTCTTCT
GTAACGCTCTGCCTTGTAGGTCCATGGGCCTGGAAATCCCAGCTTGAAAAAGCAGGTTGGGAGCAACTACAGGAGTTACC
CTGGATTGCTACCCCAGAAAAATGCTCCTTTACTGCTATAACAAAGTCTTTCTTTGAACGACATGATGTCACTGTAAAGG
CATCCATTATAGTTGATCAGGAAAAAACGCTAGCTGAATTGGTAGTAATGGGAGCAGGGATAACTCTGATGAGAGAAGAT
ATCGCTCTAGCTCTTCAGGATAATGAGGAGATTTATATCTGGCCGGGAGAAAAGACAGTGAGCCAGCTTTGTTTCCTTTG
GGATCGTGAAAAAGAGAAATCTTCTGTCTTATCTGCACTAATTGCAACGGTGAAAGATATCTGGCAAGTGCCAGCTTGA

Upstream 100 bases:

>100_bases
TTTTAACGAAGATATAGGAAAGCCGATAAATAAAGATTACTATAAAATCCATATTAATAAGAAGCAGTAATTTTTCCTGC
CGATTCGAACGGAGGATACA

Downstream 100 bases:

>100_bases
TTATCATAACTTCAATTCATATTGAAGATAACATTGCAACATCAGGCCAGGGTAAGAGAAAGTAGTCTGACTCTCAGGAA
TATGAATTATATTATAAGAA

Product: transcriptional regulator, LysR family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MDLNQLKTFVTVAETASLTRAAGLLYLSQPAVSAHIKQLETEFNVKLFKRTPRGMELTSTGLIMRDEARLALDAANKFVN
KARTLHNIENCALGTISVPVILNLPLVLSQLRLRHQNVSLTIQQNISGYIIEDIKNGKLDAGFVIGEVNDSQLGIIPVSS
VTLCLVGPWAWKSQLEKAGWEQLQELPWIATPEKCSFTAITKSFFERHDVTVKASIIVDQEKTLAELVVMGAGITLMRED
IALALQDNEEIYIWPGEKTVSQLCFLWDREKEKSSVLSALIATVKDIWQVPA

Sequences:

>Translated_292_residues
MDLNQLKTFVTVAETASLTRAAGLLYLSQPAVSAHIKQLETEFNVKLFKRTPRGMELTSTGLIMRDEARLALDAANKFVN
KARTLHNIENCALGTISVPVILNLPLVLSQLRLRHQNVSLTIQQNISGYIIEDIKNGKLDAGFVIGEVNDSQLGIIPVSS
VTLCLVGPWAWKSQLEKAGWEQLQELPWIATPEKCSFTAITKSFFERHDVTVKASIIVDQEKTLAELVVMGAGITLMRED
IALALQDNEEIYIWPGEKTVSQLCFLWDREKEKSSVLSALIATVKDIWQVPA
>Mature_292_residues
MDLNQLKTFVTVAETASLTRAAGLLYLSQPAVSAHIKQLETEFNVKLFKRTPRGMELTSTGLIMRDEARLALDAANKFVN
KARTLHNIENCALGTISVPVILNLPLVLSQLRLRHQNVSLTIQQNISGYIIEDIKNGKLDAGFVIGEVNDSQLGIIPVSS
VTLCLVGPWAWKSQLEKAGWEQLQELPWIATPEKCSFTAITKSFFERHDVTVKASIIVDQEKTLAELVVMGAGITLMRED
IALALQDNEEIYIWPGEKTVSQLCFLWDREKEKSSVLSALIATVKDIWQVPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=261, Percent_Identity=24.5210727969349, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI157672245, Length=170, Percent_Identity=31.7647058823529, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=27.1276595744681, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=274, Percent_Identity=27.7372262773723, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=28.1124497991968, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=156, Percent_Identity=32.6923076923077, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=302, Percent_Identity=24.8344370860927, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=26.1363636363636, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=151, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32455; Mature: 32455

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNQLKTFVTVAETASLTRAAGLLYLSQPAVSAHIKQLETEFNVKLFKRTPRGMELTST
CCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCEEECC
GLIMRDEARLALDAANKFVNKARTLHNIENCALGTISVPVILNLPLVLSQLRLRHQNVSL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHCEECEEECHHHHHHHHHHHHHHHHHCCEEE
TIQQNISGYIIEDIKNGKLDAGFVIGEVNDSQLGIIPVSSVTLCLVGPWAWKSQLEKAGW
EEECCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEECCHHHHHHHHHHHH
EQLQELPWIATPEKCSFTAITKSFFERHDVTVKASIIVDQEKTLAELVVMGAGITLMRED
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCEEEECC
IALALQDNEEIYIWPGEKTVSQLCFLWDREKEKSSVLSALIATVKDIWQVPA
EEEEEECCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLNQLKTFVTVAETASLTRAAGLLYLSQPAVSAHIKQLETEFNVKLFKRTPRGMELTST
CCHHHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCEEECC
GLIMRDEARLALDAANKFVNKARTLHNIENCALGTISVPVILNLPLVLSQLRLRHQNVSL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHCEECEEECHHHHHHHHHHHHHHHHHCCEEE
TIQQNISGYIIEDIKNGKLDAGFVIGEVNDSQLGIIPVSSVTLCLVGPWAWKSQLEKAGW
EEECCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEECCHHHHHHHHHHHH
EQLQELPWIATPEKCSFTAITKSFFERHDVTVKASIIVDQEKTLAELVVMGAGITLMRED
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCEEEECC
IALALQDNEEIYIWPGEKTVSQLCFLWDREKEKSSVLSALIATVKDIWQVPA
EEEEEECCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]