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The map label for this gene is proB [H]

Identifier: 194737323

GI number: 194737323

Start: 376520

End: 377623

Strand: Direct

Name: proB [H]

Synonym: SeSA_A0371

Alternate gene names: 194737323

Gene position: 376520-377623 (Clockwise)

Preceding gene: 194738232

Following gene: 194736743

Centisome position: 8.0

GC content: 56.16

Gene sequence:

>1104_bases
ATGAGTGACAGCCAGACGCTGGTCGTAAAACTCGGCACCAGCGTGTTAACAGGCGGATCGCGCCGCCTGAACCGTGCCCA
TATCGTAGAACTTGTCCGCCAGTGCGCGCAGCTACACGCCGCGGGACATCGTATTGTTATTGTTACCTCTGGCGCGATTG
CCGCCGGACGTGAACATCTCGGCTACCCGGAACTGCCGGCAACGATCGCCTCTAAACAGCTTCTGGCGGCGGTAGGGCAA
AGCCGCCTGATTCAACTGTGGGAGCAGTTGTTTTCAATTTACGGTATCCATATCGGGCAAATGCTGTTGACGCGTGCGGA
TATGGAAGACAGAGAGCGCTTTCTGAACGCCCGCGATACGCTGCGTGCATTACTGGATAACCATATTGTGCCGGTTATCA
ATGAAAATGATGCCGTTGCGACAGCGGAAATTAAAGTGGGCGATAACGATAACCTCTCCGCGCTGGCGGCGATCCTGGCA
GGCGCGGATAAACTGCTGCTGCTGACCGATCAGCAAGGATTATTTACCGCCGATCCGCGTAGCAATCCGCAGGCGGAGCT
TATCAAAGATGTGTATGGCGTTGACGATGCGCTGCGCTCTATCGCGGGAGACAGTGTTTCCGGACTGGGGACCGGTGGTA
TGAGCACTAAGCTTCAGGCGGCAGACGTGGCCTGTCGCGCGGGTATTGATACGATTATTGCCTCAGGCAGTAAACCGGGC
GTGATCGGCGATGTGATGGAGGGGATTTCCGTCGGCACCCGTTTTCATGCGCAAGCCTCGCCGCTGGAGAACCGCAAACG
TTGGATCTTCGGGGCGCCGCCGGCAGGCGAAATTACGGTTGATGAAGGCGCGACCGCGGCAATGCTGGAACGTGGCAGTT
CACTTCTGCCAAAAGGCATCAAAAGCGTGACGGGCAATTTCTCGCGGGGGGAGGTCATCAGAATTTGTAACCTGCAAGGG
CGTGATATCGCTCATGGCGTCAGCCGTTATAACAGTGATGCTCTGCGGCGGATAGCCGGCCACCACTCTCAACAAATCGA
TGCGATTCTGGGCTATGAATATGGCCCGGTCGCTGTTCATCGTGATGACATGATTACTCGATAA

Upstream 100 bases:

>100_bases
ACAAAATTTCACGCTACGCGTACAGGTTGTTGGCAACCGGAGCCAGTCCTGCTAAAACGTTCATTTGGTCTTTATTTCTT
ATTTTGAACGGCAGAGAATC

Downstream 100 bases:

>100_bases
GGAACAGGCATATGCTGGAACAAATGGGCATTGCTGCTAAAGCGGCGTCGTATAAGCTGGCGCTGCTCTCCAGCTGCGAA
AAAAATCGCGTGCTGGAAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDTLRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQQGLFTADPRSNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPG
VIGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGIKSVTGNFSRGEVIRICNLQG
RDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVHRDDMITR

Sequences:

>Translated_367_residues
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDTLRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQQGLFTADPRSNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPG
VIGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGIKSVTGNFSRGEVIRICNLQG
RDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVHRDDMITR
>Mature_366_residues
SDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQS
RLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDTLRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAG
ADKLLLLTDQQGLFTADPRSNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPGV
IGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGIKSVTGNFSRGEVIRICNLQGR
DIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVHRDDMITR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=34.0136054421769, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=33.7837837837838, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=96.7302452316076, Blast_Score=724, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=33.0882352941176, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=33.0882352941176, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=34.5323741007194, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=34.9282296650718, Blast_Score=216, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=34.2372881355932, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39141; Mature: 39010

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLFTADPR
HHHHHHCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
SNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCHHHHHCCCCCCC
VIGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGI
HHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCCCCHHHH
KSVTGNFSRGEVIRICNLQGRDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
RDDMITR
CCCCCCC
>Mature Secondary Structure 
SDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
CCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLFTADPR
HHHHHHCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
SNPQAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCHHHHHCCCCCCC
VIGDVMEGISVGTRFHAQASPLENRKRWIFGAPPAGEITVDEGATAAMLERGSSLLPKGI
HHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCCCCHHHH
KSVTGNFSRGEVIRICNLQGRDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
RDDMITR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA