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The map label for this gene is murE [H]

Identifier: 194737109

GI number: 194737109

Start: 143371

End: 144858

Strand: Direct

Name: murE [H]

Synonym: SeSA_A0139

Alternate gene names: 194737109

Gene position: 143371-144858 (Clockwise)

Preceding gene: 194734823

Following gene: 194736510

Centisome position: 3.04

GC content: 59.01

Gene sequence:

>1488_bases
GTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGGCTGGACTACCCGCGCGAGAACTGCGAGAGATGACGCT
CGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCAGTGGTGGGTCATCAGGCGGACGGGCGTCGATATATCCCGC
AGGCGATAGCGCAAGGCGTAGCTGCCATTATTGCAGAGGCGAAAGACGAGGCGAGCGACGGTGAGATTCGCGAAATGCAC
GGCGTACCGGTCGTCTATCTCAGCCAGCTCAATGAGCGTTTATCTGCGCTGGCGGGCCGCTTTTACCATGAGCCATCTGA
AAACATGCGTCTGGTGGCGGTGACCGGCACCAATGGCAAGACCACCACCACCCAACTACTGGCGCAGTGGAGCCAGTTGC
TCGGCGAAACCAGCGCAGTGATGGGGACGGTAGGTAACGGACTGTTGGGTAAAGTGATCCCGACGGAGAACACAACCGGT
TCCGCAGTGGATGTTCAGCATGTGCTGGCCAGTCTGGTTGCGCAGGGCGCGACCTTCGGCGCGATGGAAGTCTCTTCTCA
CGGCCTGGTGCAGCATCGCGTGGCAGCGCTGAAATTTGCCGCCTCCGTGTTTACTAATTTGAGCCGCGACCATCTCGACT
ATCATGGCGATATGGCGCATTACGAGGCGGCAAAATGGATGCTTTATTCCACCCACCATCACGGTCAGGCAATCGTCAAC
GCCGATGATGAAGTTGGACGCCGCTGGCTGGCGTCGCTACCCGATGCGGTCGCGGTATCAATGGAAGGGCATATCAACCC
TAACTGTCACGGTCGTTGGCTGAAAGCTGAGGCGGTGGAGTACCACGACCGCGGAGCGACGATTCGTTTTGCTTCAAGCT
GGGGTGAAGGCGAAATCGAAAGCCGCCTGATGGGCGCGTTTAACGTCAGCAACTTACTGCTGGCATTGGCGACGCTGCTG
GCGCTGGGCTATCCGTTAACGGATTTGCTGAAAACCGCCGCGCGTTTGCAGCCGGTTTGCGGGCGTATGGAAGTGTTCAC
TGCGCCTGGTAAACCGACGGTGGTGGTGGATTACGCGCACACGCCGGATGCGCTGGAAAAAGCGTTGCAGGCGGCGCGCC
TGCACTGCGCCGGAAAATTGTGGTGCGTCTTTGGCTGTGGCGGGGATCGTGACAAAGGTAAGCGCCCACTCATGGGGGCC
ATTGCCGAAGAATTCGCGGATATCGTCGTGGTGACTGACGATAACCCGCGTACCGAGGAGCCGCGCGCCATTATCAACGA
TATTCTGGCCGGAATGCTGGACGCCGGGCAGGTCAGGGTAATGGAAGGCCGCGCTGAGGCGGTAACCAATGCCATTATGC
AGGCAAAAGACAATGACGTCGTGCTGATTGCAGGTAAAGGGCACGAGGATTACCAGATTGTCGGCACGCAGCGTCTTGAT
TATTCAGACCGCGTGACCGCAGCGCGTTTGCTGGGGGTGATCGCATGA

Upstream 100 bases:

>100_bases
GCCATCATGGGCGGCGTTCTGCGCACCATGAACATCGAACCGGACGCGCTGGCAACGGGCGAAAAAAATGAATTCGTAAT
TAATCAAGGCGAGGGAACAG

Downstream 100 bases:

>100_bases
TTAGCGTAACGCTCAGCAAAATTGCCGATGTCCTTGGCGCCGAACACCGCGGGGCGGATCTCACGCTCGATACGGTCATT
ACCGACACACGGAAAGTGAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MADRNLRDLLAPWVAGLPARELREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGVAAIIAEAKDEASDGEIREMH
GVPVVYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
SAVDVQHVLASLVAQGATFGAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVN
ADDEVGRRWLASLPDAVAVSMEGHINPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIESRLMGAFNVSNLLLALATLL
ALGYPLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGA
IAEEFADIVVVTDDNPRTEEPRAIINDILAGMLDAGQVRVMEGRAEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLD
YSDRVTAARLLGVIA

Sequences:

>Translated_495_residues
MADRNLRDLLAPWVAGLPARELREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGVAAIIAEAKDEASDGEIREMH
GVPVVYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
SAVDVQHVLASLVAQGATFGAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVN
ADDEVGRRWLASLPDAVAVSMEGHINPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIESRLMGAFNVSNLLLALATLL
ALGYPLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGA
IAEEFADIVVVTDDNPRTEEPRAIINDILAGMLDAGQVRVMEGRAEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLD
YSDRVTAARLLGVIA
>Mature_494_residues
ADRNLRDLLAPWVAGLPARELREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGVAAIIAEAKDEASDGEIREMHG
VPVVYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTGS
AVDVQHVLASLVAQGATFGAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVNA
DDEVGRRWLASLPDAVAVSMEGHINPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIESRLMGAFNVSNLLLALATLLA
LGYPLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGAI
AEEFADIVVVTDDNPRTEEPRAIINDILAGMLDAGQVRVMEGRAEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLDY
SDRVTAARLLGVIA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=495, Percent_Identity=91.7171717171717, Blast_Score=887, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53287; Mature: 53156

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRNLRDLLAPWVAGLPARELREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGV
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
AAIIAEAKDEASDGEIREMHGVPVVYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGK
HHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTGSAVDVQHVLASLVAQGATFG
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCCCC
AMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVN
EEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHEEEEEECCCCCEEEC
ADDEVGRRWLASLPDAVAVSMEGHINPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIE
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHCCCCCCEEEEECCCCCCHHH
SRLMGAFNVSNLLLALATLLALGYPLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECC
TPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCC
PRAIINDILAGMLDAGQVRVMEGRAEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLD
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECEECC
YSDRVTAARLLGVIA
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
ADRNLRDLLAPWVAGLPARELREMTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHH
AAIIAEAKDEASDGEIREMHGVPVVYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGK
HHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTGSAVDVQHVLASLVAQGATFG
HHHHHHHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCCCC
AMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVN
EEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHEEEEEECCCCCEEEC
ADDEVGRRWLASLPDAVAVSMEGHINPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIE
CCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEHHHHHCCCCCCEEEEECCCCCCHHH
SRLMGAFNVSNLLLALATLLALGYPLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAH
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECC
TPDALEKALQAARLHCAGKLWCVFGCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEE
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCC
PRAIINDILAGMLDAGQVRVMEGRAEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLD
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECEECC
YSDRVTAARLLGVIA
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]