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The map label for this gene is ilvI [H]

Identifier: 194735986

GI number: 194735986

Start: 135698

End: 137452

Strand: Direct

Name: ilvI [H]

Synonym: SeSA_A0132

Alternate gene names: 194735986

Gene position: 135698-137452 (Clockwise)

Preceding gene: 194735367

Following gene: 194734361

Centisome position: 2.88

GC content: 54.19

Gene sequence:

>1755_bases
GTGATGACAGGTATAAAGTGGAGGCAAGCCGTGGAGATGTTGTCTGGAGCCGAGATGATCGTCCGATCGCTTATCGATCA
AGGCGTAAAGCAGGTGTTCGGCTATCCTGGAGGCGCAGTGCTTGATATTTATGATGCGCTACATACCGTTGGAGGGATCG
ATCATGTGCTGGTGCGTCATGAGCAGGCCGCGGTGCATATGGCGGATGGCCTGGCGCGAGCTACTGGCGAGGTTGGCGTG
GTGCTGGTGACTTCCGGACCGGGAGCGACCAATGCGATTACCGGGATTGCTACGGCCTATATGGATTCTATTCCGCTGGT
GATTCTTTCCGGCCAGGTCGCCACCTCATTGATTGGCTATGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCCCGAC
CGGTGGTCAAACATAGCTTCCTGGTCAAACAGACGGAAGATATTCCCCAGGTGCTTAAAAAGGCCTTCTGGCTGGCGGCA
AGCGGACGTCCTGGGCCGGTGGTCGTGGATTTGCCAAAAGATATTTTGAATCCGGCGAAAAAAATGCCGTATGCCTGGCC
GGAAACGGTCAGTATGCGCTCTTACAATCCCACAACATCAGGTCATAAAGGACAAATTAAGCGTGCCTTGCAAACGCTGG
CGTCGGCAAAAAAGCCGGTGGTCTATGTTGGGGGAGGGGCGATAAGCGCGGCCTGCTATACGCCGCTGCGCCACATCATT
GAAACCTTTAACCTGCCGGTGGTCTCTTCATTAATGGGACTGGGCGCGTTTCCTGCTACTCATCGCCAGTCGTTAGGAAT
GCTGGGAATGCATGGCACTTATGAAGCCAATATGACCATGCATAACGCCGACGTGATTTTCGCTGTTGGCGTACGATTTG
ACGACCGCACGACGAATAATCTCGCTAAATATTGTCCGAACGCGACGGTATTACACATCGACATTGACCCGACGTCCATA
TCCAAAACGGTTAACGCTGATATCCCGGTGGTGGGCGATGCCCGCCTGGTACTGGAACAAATGCTGGAGCTACTGGCGCA
GGATGCGCCGTCTCAGCCGCAGGATGATATTCGTGACTGGTGGCAGCAGATAGAGATCTGGCGCGCCCGTCAGTGCCTGA
AATATGACGCGGAAAGCGAAAGCATTAAACCCCAGGCGGTGATTGAAACCCTCTGGCGTCTGACGAAGGGCGACGCTTAC
GTGACGTCCGATGTAGGACAACACCAGATGTTTGCCGCCCTCTACTACCCGTTCGATAAGCCACGTCGCTGGATTAATTC
CGGCGGGCTCGGCACGATGGGTTTTGGCCTACCGGCTGCGCTGGGCGTTAAAATGGCCCTGCCGAAAGAAATGGTGGTCT
GCGTGACCGGCGATGGCAGCATACAGATGAACATTCAGGAGCTGTCGACAGCCTTACAGTATGAACTGCCGGTACTGGTT
CTGAACCTGAACAACCGTTATCTGGGGATGGTGAAACAGTGGCAGGATATGATCTATTCTGGTCGCCATTCTCAGTCTTA
TATGCAGTCGTTACCGGATTTTGTGCGCCTGGCGGAAGCATATGGCCATGTCGGGCTGCAGATTAACCGTCCGGATGAGC
TGGAAAGTAAACTCAGCGAAGCCCTTGAGCATGTGCGTAATAACCGACTGGTGTTCGTCGATGTCACCGTTGATGGCAGC
GAGCATGTCTATCCAATGCAGATTCGCGGGGGCGGGATGGACGAAATGTGGTTAAGCAAAACGGAGAGGACCTGA

Upstream 100 bases:

>100_bases
TTCTCTTCTTGCCTCCTGATTATTCTAACCTTCCCCATCTTTACGTCATTCCACCCAATTGCCAGGCGTGATAGCGAGCG
GTTAAGGTGTCCGTCAAACC

Downstream 100 bases:

>100_bases
TGATGCGCCGGATTTTATCGGTATTACTGGAAAACGAATCTGGGGCGTTATCGCGGGTTATCGGCCTCTTTTCGCAACGC
GGATATAATATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MMTGIKWRQAVEMLSGAEMIVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGV
VLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAA
SGRPGPVVVDLPKDILNPAKKMPYAWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYTPLRHII
ETFNLPVVSSLMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSI
SKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIEIWRARQCLKYDAESESIKPQAVIETLWRLTKGDAY
VTSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLV
LNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGS
EHVYPMQIRGGGMDEMWLSKTERT

Sequences:

>Translated_584_residues
MMTGIKWRQAVEMLSGAEMIVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGV
VLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAA
SGRPGPVVVDLPKDILNPAKKMPYAWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYTPLRHII
ETFNLPVVSSLMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSI
SKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIEIWRARQCLKYDAESESIKPQAVIETLWRLTKGDAY
VTSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLV
LNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGS
EHVYPMQIRGGGMDEMWLSKTERT
>Mature_584_residues
MMTGIKWRQAVEMLSGAEMIVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGV
VLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAA
SGRPGPVVVDLPKDILNPAKKMPYAWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYTPLRHII
ETFNLPVVSSLMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSI
SKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIEIWRARQCLKYDAESESIKPQAVIETLWRLTKGDAY
VTSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLV
LNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGS
EHVYPMQIRGGGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=26.9366197183099, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI21361361, Length=590, Percent_Identity=26.6101694915254, Blast_Score=165, Evalue=9e-41,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=92.1602787456446, Blast_Score=1108, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=39.8945518453427, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=34.5149253731343, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=29.1071428571429, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.3597122302158, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=27.2727272727273, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=27.0944741532977, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=579, Percent_Identity=25.73402417962, Blast_Score=153, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.8816466552316, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=25.2631578947368, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922626, Length=505, Percent_Identity=29.1089108910891, Blast_Score=191, Evalue=2e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64168; Mature: 64168

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTGIKWRQAVEMLSGAEMIVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRH
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
EQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGY
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
DAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPAK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHH
KMPYAWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYTPLRHII
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ETFNLPVVSSLMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNN
HHHCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCEECCEEEECCCEEEEEEEEECCCCHHH
LAKYCPNATVLHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDW
HHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
WQQIEIWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHH
PRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLV
HHHHHCCCCCCCCCCCCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEE
LNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSE
EECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHH
ALEHVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MMTGIKWRQAVEMLSGAEMIVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRH
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
EQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGY
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
DAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPAK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHH
KMPYAWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYTPLRHII
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ETFNLPVVSSLMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNN
HHHCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCEECCEEEECCCEEEEEEEEECCCCHHH
LAKYCPNATVLHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDW
HHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
WQQIEIWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHH
PRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLV
HHHHHCCCCCCCCCCCCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEE
LNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSE
EECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHH
ALEHVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
HHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]