Click here to switch to the map view.

The map label for this gene is pflB [H]

Identifier: 194735585

GI number: 194735585

Start: 3313966

End: 3316260

Strand: Reverse

Name: pflB [H]

Synonym: SeSA_A3432

Alternate gene names: 194735585

Gene position: 3316260-3313966 (Counterclockwise)

Preceding gene: 194736109

Following gene: 194737040

Centisome position: 70.42

GC content: 54.9

Gene sequence:

>2295_bases
ATGAAGGTAAATATCGATACCAGCGATATGCTGTATGCCGAAGCATGGCGTGATTTTAAAGGTACGGACTGGAAAGAAGA
AATTAATGTCCGTGATTTTATTCAGCATAACTATACGCCCTATGAGGGGGATGAATCTTTTCTCGCCGACGCGACGCCAG
CGACCACGGCGCTGTGGGAAAAGGTGATGGCGGGAATACGTATCGAGAACGCGACCCATGCGCCGGTGGATTTCGATACC
AACATCGCGACGACGATTACCGCGCACGATGCGGGATATATTGAAAAAGATCTGGAAAAAATCGTCGGTTTACAAACGGA
TAAGCCGCTTAAACGCGCTTTGCATCCGTTTGGCGGCGTTAACATGATCAAAAGTTCATTCCACGCCTATGGCCGCGAAA
TGGACGCCGATTTTGAATATACCTTTACCGATCTGCGGAAAACCCATAATCAGGGCGTATTCGATGTTTACTCGCCGGAT
ATGCTGCGCTGCCGTAAATCCGGCGTATTAACCGGTTTGCCGGATGGTTATGGTCGCGGGCGCATTATTGGCGATTATCG
TCGCGTCGCGCTGTATGGCATCCGTTATCTGGTGCGCGAACGCGAGCTGCAATTCGCCGATCTGCAGTCGAATCTGGAGC
AGGGACAGAATCTTGAGGCGACGATTCGCCTGCGTGAGGAGTTGGCGGAGCATCGTCGCGCGCTGCTGCAAATGCAGGAG
ATGGCGGCGAAATACGGCTACGATATCTCCCGCCCGGCGCGCAACGCGCAGGAAGCGGTACAGTGGCTCTACTTCGCTTA
TCTGGCGGCGGTAAAATCGCAAAATGGCGGTGCAATGTCGCTGGGAAGAACCGCCTCGTTCCTTGATATCTACATTGAGC
GCGATTTCAACGCGGGCCTTCTGACAGAGCAGCAGGCGCAGGAACTGATCGACCATTTCATCATGAAGATCCGCATGGTG
CGCTTCCTGCGCACGCCGGAATTTGACTCGCTCTTTTCCGGTGATCCGATCTGGGCCACCGAAGTTATTGGCGGGATGGG
GCTTGATGGCCGCACGCTGGTGACGAAGAACTCCTTCCGTTATCTGCATACCCTGCACACGATGGGGCCAGCGCCGGAGC
CGAACCTGACCATACTGTGGTCAGAAGCGTTGCCGGTGGCATTCAAAAAATATGCTGCGCAGGTCTCCATCGTGACCTCA
TCTTTGCAGTATGAAAACGACGATTTAATGCGTACCGACTTTAACAGCGACGATTACGCCATCGCTTGTTGCGTCAGCCC
GATGGTCATCGGTAAGCAGATGCAGTTCTTCGGCGCGCGCGCCAACCTTGCCAAAACGTTGCTGTACGCTATCAATGGCG
GCGTTGACGAGAAACTGAAAATCCAGGTTGGGCCGAAAACCGCGCCGTTAACGGATGAGGTGCTGGATTACGACGCGGTA
ATGGAGAGCCTCGACCATTTTATGGACTGGCTGGCGGTGCAGTACATTAGCGCGCTGAACATCATTCACTACATGCACGA
TAAGTACAGCTATGAAGCTTCGCTGATGGCGCTGCACGACCGTGATGTGTATCGGACGATGGCGTGCGGCATCGCCGGAC
TGTCGGTGGCGGCGGATTCCCTGTCCGCTATCAGGTACGCTCAGGTGAAGCCGATCCGCAACGAAAACGGCCTTGCCATC
GACTTCGCTATTGAAGGCGAGTATCCGCAGTACGGCAACAACGATGAGCGCGTGGACAGCATCGCCTGCGACCTGGTGAA
ACGCTTTATGCAAAAAATTAGTGTGTTGCCGACGTATCGCAATGCGGTGCCGACGCAGTCCATTTTGACCATCACCTCGA
ACGTCGTGTACGGGCAGAAGACCGGCAATACGCCGGATGGACGCCGCGCCGGAACGCCGTTCGCGCCGGGGGCGAACCCG
ATGCACGGTCGTGACCGCAAAGGGGCGGTGGCCTCGCTGACGTCGGTGGCGAAACTGCCGTTTACCTACGCCAAAGACGG
TATTTCGTACACCTTCTCGATTGTGCCTGCGGCGCTCGGAAAAGAAGATGCGGTGCGGAAAACCAATCTGGTTGGCCTGC
TGGACGGCTATTTCCATCATGAAGCACAGGTAGAAGGCGGGCAGCATCTGAACGTCAATGTCATGAACCGTGAAATGTTG
TTGGATGCGATTGAACATCCGGAAAACTACCCGAACCTGACGATCCGCGTTTCCGGCTATGCCGTGCGCTTCAATGCGCT
GACTCGTGAGCAGCAACAGGACGTTATTTCGCGCACCTTTACTCAGGCGATGTAA

Upstream 100 bases:

>100_bases
AGAGAAAATGATTGCCCTTGATGCCATTCATTTAGGCAACGTGAAAGCACCAGTGGAATTTGCATAACTTAACGGCTGTT
CTGTAGAGAGATTATTTTTC

Downstream 100 bases:

>100_bases
CGTGAACGGGGCGATCGTGTGGATCGTCCCGCGACGGATCTTCACTGAACGGTAATCTTAGGGGGAGATATGATTAGCGC
ATTTGATATTTTTAAGATTG

Product: formate acetyltransferase

Products: CoA; pyruvate

Alternate protein names: Keto-acid formate-lyase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVRDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKDLEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLEQGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRNENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM

Sequences:

>Translated_764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVRDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKDLEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLEQGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRNENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
>Mature_764_residues
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVRDFIQHNYTPYEGDESFLADATPATTALWEKVMAGIRIENATHAPVDFDT
NIATTITAHDAGYIEKDLEKIVGLQTDKPLKRALHPFGGVNMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSNLEQGQNLEATIRLREELAEHRRALLQMQE
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGLLTEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADSLSAIRYAQVKPIRNENGLAI
DFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=93.4554973821989, Blast_Score=1501, Evalue=0.0,
Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=79.1223404255319, Blast_Score=1284, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=627, Percent_Identity=27.1132376395534, Blast_Score=174, Evalue=3e-44,
Organism=Escherichia coli, GI1790388, Length=641, Percent_Identity=25.4290171606864, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=73.4375, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: 2.3.1.54

Molecular weight: Translated: 85850; Mature: 85850

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVRDFIQHNYTPYEGDESFLADATPATTALWE
CEEECCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KVMAGIRIENATHAPVDFDTNIATTITAHDAGYIEKDLEKIVGLQTDKPLKRALHPFGGV
HHHHCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCH
NMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQSNLEQGQNLEATIRLREELAEHRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LTEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEEECCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIRYAQVKPIRNENGLAIDFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEECHHHHH
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
HHHHHCCCCCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVNIDTSDMLYAEAWRDFKGTDWKEEINVRDFIQHNYTPYEGDESFLADATPATTALWE
CEEECCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KVMAGIRIENATHAPVDFDTNIATTITAHDAGYIEKDLEKIVGLQTDKPLKRALHPFGGV
HHHHCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCH
NMIKSSFHAYGREMDADFEYTFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQSNLEQGQNLEATIRLREELAEHRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAAKYGYDISRPARNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFNAGL
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LTEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEALPVAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEEECCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLTDEVLDYDAV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHH
MESLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIRYAQVKPIRNENGLAIDFAIEGEYPQYGNNDERVDSIACDLVKRFMQKISVLPTYR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDAVRKTNLVGLLDGYFHHEAQVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEEECHHHHH
LDAIEHPENYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAM
HHHHHCCCCCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901 [H]