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The map label for this gene is dusC [H]

Identifier: 194735562

GI number: 194735562

Start: 2291190

End: 2292128

Strand: Reverse

Name: dusC [H]

Synonym: SeSA_A2413

Alternate gene names: 194735562

Gene position: 2292128-2291190 (Counterclockwise)

Preceding gene: 194735226

Following gene: 194737549

Centisome position: 48.67

GC content: 57.29

Gene sequence:

>939_bases
ATGCGTGTTTTACTGGCGCCGATGGAAGGCGTGCTCGACGCGTTAGTGCGCGAGCTGCTGACCGAAGTGAATGATTACGA
TCTCTGCATCACCGAATTTGTGCGCGTGGTGGATCAGCTGCTGCCGGTAAAAGTGTTTCATCGCATCTGCCCGGAGTTGC
ATCATGCCAGCCGCACGCCGTCCGGCACGCCGGTGCGTATTCAGCTTCTGGGCCAGCATCCGCAGTGGCTGGCGGAAAAC
GCCGCGCGGGCGACGGCGTTGGGATCGTATGGCGTGGACCTGAACTGCGGCTGTCCGTCAAAAGTGGTGAACGGCAGCGG
CGGCGGCGCGACATTGCTCAAAGATCCCGAACTCATCTATCAGGGCGCGAAAGCGATGCGGGCCGCGGTACCGTCGCATC
TTCCGGTGACGGTAAAAGTGCGTCTCGGCTGGGATAGCGGCGATAGAAAATTTGAAATCGCCGATGCGGTGCAGCAGGCC
GGCGCCAGTGAACTGGTGGTGCATGGCCGTACCAAAGCGCAGGGCTACCGCGCCGAGCATATCGACTGGCAGGCGATCGG
TGAAATACGCCAGCGTCTGACTATTCCGGTTATCGCGAATGGCGAAATCTGGGACTGGCAGAGCGCGCAGGCATGTATGG
CGGCCAGCGGCTGCGATGCGGTGATGATTGGCCGTGGGGCGTTAAATATTCCTAACCTGAGCCGGGTGGTGAAGTATAAC
GAACCGCGTATGCCGTGGCCGGAAGTGGTTGCATTATTACAAAAATATACCCGACTGGAAAAGCAGGGCGATACCGGTTT
ATACCATGTCGCGCGTATTAAACAGTGGTTGGGATATTTACGTAAGGAATATATTGAGGCGACGGAACTCTTTCAGTCGA
TTCGGGCGTTAAACCGTTCGTCCGAGATTGCGCGGGCGATTCAGGCTATTAAAATCTAA

Upstream 100 bases:

>100_bases
CGTGACGTAGCGTGGCGTTTGCGCCGCCACCCGGCAAAAAGTTTTGCTCATCGCAGAGAGGCGTTATGATAGCGCCTCTT
TTTTTGCTGTGGATACCGAT

Downstream 100 bases:

>100_bases
TCTCTACAGCGTCCAGAACGTGTGCCGGACAGATACTATCGCTGCCCGGCGCAGGAATCAGAAAATCATCTTAAACACGC
CCGTTACCACCAACAGCCCA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMAASGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI

Sequences:

>Translated_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMAASGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI
>Mature_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMAASGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=29.004329004329, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=287, Percent_Identity=27.5261324041812, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=177, Percent_Identity=30.5084745762712, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI239788462, Length=177, Percent_Identity=30.5084745762712, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=90.3846153846154, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=29.5031055900621, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693211, Length=249, Percent_Identity=27.710843373494, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=32.5581395348837, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=144, Percent_Identity=34.7222222222222, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=202, Percent_Identity=28.7128712871287, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585320, Length=209, Percent_Identity=32.0574162679426, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=30.1675977653631, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=30.1675977653631, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34531; Mature: 34531

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMAASGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARAIQAIKI
HHHHHHHHHHCC
>Mature Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEIWDWQSAQACMAASGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARAIQAIKI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]