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The map label for this gene is alkB [H]

Identifier: 194735473

GI number: 194735473

Start: 2368259

End: 2368909

Strand: Reverse

Name: alkB [H]

Synonym: SeSA_A2490

Alternate gene names: 194735473

Gene position: 2368909-2368259 (Counterclockwise)

Preceding gene: 194735664

Following gene: 194736104

Centisome position: 50.31

GC content: 59.29

Gene sequence:

>651_bases
ATGCTGGATCTATTTGCTGATGAAGCGCCCTGGCAAGAGCCCCTGGCGCCTGGCGCGGTGGTGTTGCGCCGCTTTGCGTT
TCGCGCGGCGCAGTCGCTATTAGACGATATCGGGTTTGTCGCCAGTCAGTCTCCGTTTCGTCAGATGGTGACGCCGGGCG
GCTACACCATGTCGGTGGCGATGACCAACTGCGGCGCGTTAGGGTGGACCACCGATCGTCACGGTTATTGTTATGCCGTG
CGCGATCCGTTGACCGATAAACCCTGGCCTGCGCTGCCGTTATCGTTTGCCAGCGTATGTCGTCAGGCGGCAATCGCGGC
AGGGTATGCGAGCTTCCAACCGGACGCCTGCCTGATCAATCGCTACGCGCCCGGCGCAAAACTGTCGCTGCATCAGGATA
AAGACGAGCCGGATCTGCGCGCGCCTATCGTCTCAGTTTCGTTGGGGGTGCCGGCGATTTTTCAGTTTGGCGGCCTGCGT
CGCAGCGATCCGCTCCAGCGGATCTTACTGGAGCATGGCGATATCGTGGTCTGGGGCGGCGAGTCCCGGCTGTTTTATCA
TGGTATCCAGCCGCTTAAAGCGGGCTTTCATCCCATGACCGGTGAATTTCGTTACAATCTCACCTTCCGTCAGGCGGCTG
AAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCGGGTCGTGCGTCGTGATGGCGCGCTCTCCGGTTATCGTTGGGGCGTGCGTCGAAAAGCGCAGCTATTAAAGCGAGAA
GCGCAAAAAGAGGAGTAGCA

Downstream 100 bases:

>100_bases
AAATAAGAATTATTATTGCTGTGCAGGTGGAGTTAGTTAAACTGCGGGCTGTTATTATCGGTCCGGGTTGTATGCATGGA
ACTTCTTATTCTTGTCTGGC

Product: alpha-ketoglutarate-dependent dioxygenase AlkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAIFQFGGLR
RSDPLQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE

Sequences:

>Translated_216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAIFQFGGLR
RSDPLQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
>Mature_216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAIFQFGGLR
RSDPLQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=123, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=80.5555555555556, Blast_Score=367, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45555401, Length=127, Percent_Identity=37.7952755905512, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23801; Mature: 23801

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLIN
ECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAIFQFGGLRRSDPLQRILLEHGDIVVWGG
CCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
CCEEEEECCCHHHCCCCCCCCCEEEEEEHHHHCCCC
>Mature Secondary Structure
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLIN
ECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAIFQFGGLRRSDPLQRILLEHGDIVVWGG
CCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
CCEEEEECCCHHHCCCCCCCCCEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]