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The map label for this gene is hybC [H]

Identifier: 194735258

GI number: 194735258

Start: 3214229

End: 3215932

Strand: Reverse

Name: hybC [H]

Synonym: SeSA_A3329

Alternate gene names: 194735258

Gene position: 3215932-3214229 (Counterclockwise)

Preceding gene: 194737021

Following gene: 194736032

Centisome position: 68.29

GC content: 54.93

Gene sequence:

>1704_bases
ATGAGCCAGAGAATTACTATTGATCCGGTAACCCGTATTGAGGGTCATTTACGCATTGACTGCGAAATTGAGAACGGCGT
CGTATCCAAAGCATGGGCGTCCGGCACCATGTGGCGCGGTATGGAAGAGATCGTGAAGAACCGCGATCCGCGCGATGCGT
GGATGATTGTGCAGCGTATTTGCGGCGTTTGTACGACAACTCACGCGATCTCCTCCGTTCGTGCGGCGGAAAGCGCGCTG
AATATTGATGTTCCCGTCAACGCGCAGTATATCCGTAATATTATTCTGGCGGCGCATACCACCCATGACCACATTGTCCA
TTTCTACCAGCTCTCTGCTCTGGACTGGGTGGACATTACCTCTGCGCTGAAAGCCGATCCGGAAAAAGCCTCCGCGATGC
TGGATGGCGTGTCGAGCTGGCACCTGAACAGCGCGCAAGAGTTCACGAAAGTTCAGAACAAGATCAAGGATCTTGTCGCC
AGCGGTCAGTTAGGTATTTTCGCCAATGGCTACTGGGGGCACCCGGCAATGCAGTTACCGCCGGAGGTCAACCTGATTGC
GGTCGCCCACTATCTGCAGGCGCTGGAATGTCAGCGTGACGCTAACCGCGTGGTGGCGTTGCTGGGCGGTAAATCGCCGC
ATATCCAGAACCTGGCGGTCGGTGGGGTGGCGAACCCGATCAATCTCGACAGCATCGGTACGTTGAACCTTGAACGCCTG
ATGTACATCAAATCGTTTATCGATAAGCTGAGCGATTTTGTCGAGCAGGTGTATAAAGTGGATACCGCAGTCATCGCGGC
TTACTATCCGGAATGGCTGGAGCGCGGCAAAGGCGCGGTGAACTACCTGAGCGCGCCAGAATTCCCGACCGACGGTAAAA
ACGGTAGTTTCCTGTTCCCGGGCGGTTACATTACCAATGCCGATCTGTCGACATACCGTCCGATCACCTCTCACTCCGAC
GAGTTCCTGATTAAAGGTATTCAGGAGAGCGCGAAGCACGCCTGGTATAAAGATGAAGCGCCGCAGGCACCGTGGGAAGG
CACCACGATTCCGGCTTACGACGGTTGGTCGGATGACGGTAAATACTCATGGGTCAAGTCGCCGACATTCTACGGCAAAA
CCGTCGAAGTCGGGCCGCTGGCGAACATGCTGTGTAAACTGGCTGCAGGTCGTGAATCCACCCAGACCAAGCTCAATGAA
ATCATTGCGCTTTATCAGAAGCTGACCGGCAAAACGCTGGAAATGGCGCAACTTCACTCTACGCTGGGTCGTATCATCGG
GCGTACCGTTCACTGTTGTGAACTGCAAAACGTATTGCAGGATCAATACAACGCGCTGATTGTAAATATCGGCAAAGGCG
ATTACACCACGTTTGTGAAGCCGAACATTCCGGCGACGGGCGAGTTCAAAGGCGTGGGCTTCCTGGAGGCGCCGCGCGGT
ATGCTGTCTCACTGGATGGTGATTAAAGACGGTATTATCAGTAACTATCAGGCAGTGGTGCCATCGACCTGGAACTCAGG
TCCGCGTAACTTCAACGATGACGTGGGGCCGTACGAACAGTCGCTGGTCGGTACGCCGATCGCCGATCCGGCGAAGCCGC
TGGAAGTGGTACGTACCATCCACTCCTTCGACCCTTGCATGGCGTGCGCGGTACACGTGGTGGACGCCGATGGCAATGAA
GTGGTCTCGGTGAAGGTTTTGTAA

Upstream 100 bases:

>100_bases
TGGCTATTGAGATTTGTGCATACATCGTACTCATTCGTCTACTGCCGATACTTCCTCCTTTAAAACAAAACGATCATAAT
CGTCATGAGGCGAGCAAAGC

Downstream 100 bases:

>100_bases
TGCGGATATTAGTCTTAGGGGTCGGCAATATTTTGCTGACCGATGAAGCCATCGGGGTGCGTATTGTTGAAGCGTTAGAG
CAACGATACACCCTGCCGGA

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSD
EFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNE
IIALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL

Sequences:

>Translated_567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSD
EFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNE
IIALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL
>Mature_566_residues
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESALN
IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVAS
GQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERLM
YIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSDE
FLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEI
IALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRGM
LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNEV
VSVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=93.8271604938272, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=42.4703891708968, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62425; Mature: 62294

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHAISSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVASGQLGIFANGYWGHPAMQLP
HHHHCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
GGYITNADLSTYRPITSHSDEFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDG
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEIIALYQKLTGKTLEMAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHAISSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVASGQLGIFANGYWGHPAMQLP
HHHHCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
GGYITNADLSTYRPITSHSDEFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDG
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEIIALYQKLTGKTLEMAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HHCCHHHHHEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]