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The map label for this gene is narU [H]

Identifier: 194735231

GI number: 194735231

Start: 1629942

End: 1631342

Strand: Direct

Name: narU [H]

Synonym: SeSA_A1693

Alternate gene names: 194735231

Gene position: 1629942-1631342 (Clockwise)

Preceding gene: 194735466

Following gene: 194735877

Centisome position: 34.61

GC content: 52.39

Gene sequence:

>1401_bases
GTGAGGGGTAAAATGACACGACAAAACGAGAATTATAACCGTTATCTCTTGAGTGACTGGCGACCCGAAAATCCAGCCTT
CTGGGAGAATAAAGGGAAAGGCATCGCCAGAAGAAACTTATGGATTTCAGTAAGTTGTTTGCTTCTGGCATTTTGCGTCT
GGATGTTGTTTAGCGCTGTTGCCGTCAACCTCAATAAAATTGGATTTAATTTTACCACCGACCAACTCTTTTTATTAACC
GCATTACCTTCTCTTTCCGGTGCAATATTACGCGTTCCCTACTCCTTTATGGTACCACTGTTTGGTGGCCGTAAATGGAC
CGTATTAAGTACCGTAATATTGATTATTCCCTGCGCCTGGCTGGGATTCGCCGTGCAAAATCCCGCCACCCCTTTCGGGG
TATTTATGCTCATCGCTCTGTTGTGCGGTTTTGCGGGGGCAAACTTCGCGTCCAGTATGGGCAACATCAGTTTTTTCTTT
CCAAAGGCCAGACAAGGTAGCGCGTTGGGTATTAACGGCGGACTGGGAAATCTTGGCGTCAGTGTGATGCAGTTGATTGC
GCCGCTGGTTATTTTTCTGCCGATATTTACTTTTTTAGGCGTCCGGGGCGTGCCGCAACCTGATGGCTCCCTGCTCGCCC
TCACTAACGCCGCCTGGATCTGGGTGCCGTTACTGGCTGTCGCGACGCTTGCCGCCTGGTTTGGTATGAACGATATCGGC
AGTTCAAAAGCGTCTATCGCCTCGCAATTGCCGGTGCTAAAACGTCTGCACCTGTGGCTGCTTAGCCTGCTGTACCTGGC
GACATTCGGGTCATTCATCGGCTTTTCCGCAGGTTTCGCCATGCTCGCCAAAACGCAATTTCCCGACGTAAACATTCTGC
AACTGGCGTTCTTTGGCCCCTTTATCGGCGCGCTGGCGCGATCGGCAGGCGGCGTGATCTCCGATAAATTCGGCGGCGTT
CGCGTAACGCTCATTAATTTTATCTTTATGGCGCTGTTTACCGCCCTGCTATTCCTCACGCTGCCAGGCTCCGGCGCAGG
CAGCTTTAGCGCGTTCTATCTTGTTTTTATGGGCCTGTTTTTGACAGCGGGTCTCGGCAGCGGTTCTACCTTCCAGATGA
TTGCGGTGATCTTTCGTCAGATCACGCTCTACAACGTCAAACTGCGCGGCGGGAGCGATGAGCAGGCGCAGCGCGAAGCG
GTGACGGATACCGCCGCCGCGCTCGGGTTTATCTCCGCTATTGGCGCTGTGGGCGGCTTCTTTATTCCAAAAGCGTTTGG
CACCTCTTTGGCATTGACCGGCTCGCCGGTGGGCGCCATGAAAATCTTTTTGCTGTTTTACATCGCTTGCGTCCTGCTGA
CCTGGCTGGTCTACGGGCGTCGTAAGCCTAAACAACAATAA

Upstream 100 bases:

>100_bases
TATTCTTTCACTCAATATTCTAAAAAAACTTCAATAAGTTCTCAAAAGAGCGCGTGACGCCCTGTCTCTTTTTTATGTTT
TTTTCAAATGATATGCGCAT

Downstream 100 bases:

>100_bases
CAACATACATACCTGTAGGACGGCCATCCGGCAATGGTTTAACGCCAAATCGACAGGATGGCGGAAAATTTATCGAAGCA
GGAGAAATGTCATGAGTAAA

Product: nitrite extrusion protein 2

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRGKMTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLT
ALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFF
PKARQGSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIG
SSKASIASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGV
RVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREA
VTDTAAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRRKPKQQ

Sequences:

>Translated_466_residues
MRGKMTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLT
ALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFF
PKARQGSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIG
SSKASIASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGV
RVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREA
VTDTAAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRRKPKQQ
>Mature_466_residues
MRGKMTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLT
ALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFF
PKARQGSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIG
SSKASIASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGV
RVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREA
VTDTAAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRRKPKQQ

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=85.4978354978355, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=75.6578947368421, Blast_Score=678, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50266; Mature: 50266

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKMTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAV
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AVNLNKIGFNFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAW
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGV
HHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHH
SVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
SSKASIASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FIGALARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGF
HHCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRRKPKQQ
HHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRGKMTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAV
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AVNLNKIGFNFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAW
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGV
HHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHH
SVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
SSKASIASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FIGALARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGF
HHCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRRKPKQQ
HHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7926834 [H]