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The map label for this gene is spaL

Identifier: 194735197

GI number: 194735197

Start: 2943873

End: 2945168

Strand: Reverse

Name: spaL

Synonym: SeSA_A3047

Alternate gene names: 194735197

Gene position: 2945168-2943873 (Counterclockwise)

Preceding gene: 194735438

Following gene: 194734552

Centisome position: 62.54

GC content: 54.17

Gene sequence:

>1296_bases
ATGAAAACACCTCGTTTACTGCAATATCTGGCCTACCCACAAAAAATAACCGGCCCAATTATTGAGGCGGAATTGCGCGA
TGTAGCCATTGGCGAACTGTGTGAAATACGCCGTGGCTGGCACCAAAAACAGGTTGTTGCACGTGCGCAGGTGGTTGGCT
TACAGCGGGAACGCACCGTGCTGAGCCTTATCGGCAATGCCCAGGGGCTGAGCCGCGATGTCGTGCTTTATCCCACTGGA
CGTGCGTTATCGGCGTGGGTGGGATACTCGGTATTAGGCGCGGTGTTGGATCCGACAGGGAAAATCGTTGAGCGTTTTAC
CCCTGAAGTGGCGCCGATTAGCGAAGAACGCGTTATTGATGTCGCACCGCCTTCTTACGCTTCGCGCGTTGGCGTCCGTG
AACCGCTGATTACCGGTGTGCGCGCGATTGACGGGTTATTGACCTGTGGCGTAGGCCAGCGAATGGGCATTTTTGCCTCC
GCAGGATGCGGTAAGACCATGCTGATGCATATGCTGATCGAGCAAACGGAGGCGGATGTTTTTGTTATTGGTCTTATCGG
TGAACGAGGCCGCGAGGTCACTGAATTCGTGGATATGTTGCGCGCTTCGCATAAGAAAGAAAAATGCGTGCTGGTTTTTG
CCACTTCCGATTTCCCCTCGGTCGATCGCTGCAATGCAGCGCAACTGGCGACAACCGTAGCGGAATATTTTCGCGACCAG
GGAAAACGGGTCGTGCTTTTTATCGATTCCATGACCCGTTATGCGCGTGCTTTGCGAGACGTGGCACTGGCGTCGGGAGA
GCGTCCGGCCCGTCGAGGTTATCCCGCCTCCGTATTCGATAATTTGCCCCGCTTGCTGGAACGCCCCGGGGCGACCAGCG
AGGGAAGCATTACTGCCTTTTATACGGTACTGCTGGAAAGCGAGGAAGAGGCGGACCCGATGGCGGATGAAATTCGCTCT
ATCCTTGACGGTCACCTGTATCTGAGCAGAAAGCTGGCCGGGCAGGGACATTACCCGGCAATCGATGTACTGAAAAGCGT
AAGCCGCGTTTTTGGACAAGTCACGACGCCGACACATGCTGAACAGGCATCTGCCGTGCGTAAATTAATGACGCGTCTGG
AAGAGCTCCAGCTTTTCATTGACTTGGGAGAATATCGTCCTGGCGAAAATATCGATAACGATCGGGCGATGCAGATGCGG
GATAGCCTGAAAGCCTGGTTATGCCAGCCGGTAGCGCAATATTCATCCTTTGATGACACGTTGAGCGGTATGAATGCATT
CGCTGACCAGAATTAA

Upstream 100 bases:

>100_bases
CCTTAGTGCATCCGGATTTTTTATCTGACGGTGAAAAGTTCTCTACTGCCTTGAATGGGTTTTACAACTATCTGGAAGTT
TTTAGTCGGTCGCTAATGAG

Downstream 100 bases:

>100_bases
AGTATTGCAGCGGCGCTGTACGGTATTTCATTCACAGTGTGAGTCGATATTACTTCGCTATCAGGATGAGGACCGCGGGC
TGCAGGCCGAGGAGGAGGCG

Product: ATP synthase SpaL

Products: NA

Alternate protein names: Invasion protein InvC

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTVLSLIGNAQGLSRDVVLYPTG
RALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVIDVAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFAS
AGCGKTMLMHMLIEQTEADVFVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ
GKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAFYTVLLESEEEADPMADEIRS
ILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHAEQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMR
DSLKAWLCQPVAQYSSFDDTLSGMNAFADQN

Sequences:

>Translated_431_residues
MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTVLSLIGNAQGLSRDVVLYPTG
RALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVIDVAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFAS
AGCGKTMLMHMLIEQTEADVFVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ
GKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAFYTVLLESEEEADPMADEIRS
ILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHAEQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMR
DSLKAWLCQPVAQYSSFDDTLSGMNAFADQN
>Mature_431_residues
MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTVLSLIGNAQGLSRDVVLYPTG
RALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVIDVAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFAS
AGCGKTMLMHMLIEQTEADVFVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ
GKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAFYTVLLESEEEADPMADEIRS
ILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHAEQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMR
DSLKAWLCQPVAQYSSFDDTLSGMNAFADQN

Specific function: Necessary for efficient entry of S.typhimurium into cultured epithelial cells. Probable catalytic subunit of a protein translocase. May energize the protein export apparatus encoded in the inv locus which is required for the surface presentation of determ

COG id: COG1157

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway ATPase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family

Homologues:

Organism=Homo sapiens, GI32189394, Length=358, Percent_Identity=29.0502793296089, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI50345984, Length=373, Percent_Identity=26.8096514745308, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4757810, Length=373, Percent_Identity=26.8096514745308, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI19913424, Length=265, Percent_Identity=30.5660377358491, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI19913428, Length=292, Percent_Identity=27.7397260273973, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI19913426, Length=374, Percent_Identity=25.668449197861, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1788251, Length=423, Percent_Identity=37.3522458628842, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1790170, Length=440, Percent_Identity=28.8636363636364, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790172, Length=350, Percent_Identity=25.4285714285714, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790217, Length=242, Percent_Identity=28.5123966942149, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144756, Length=326, Percent_Identity=30.0613496932515, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71988080, Length=373, Percent_Identity=26.8096514745308, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71988063, Length=373, Percent_Identity=26.8096514745308, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17570191, Length=361, Percent_Identity=26.8698060941828, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17565854, Length=283, Percent_Identity=29.3286219081272, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71988074, Length=313, Percent_Identity=28.7539936102236, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17510931, Length=360, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322581, Length=390, Percent_Identity=29.4871794871795, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319603, Length=354, Percent_Identity=26.8361581920904, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319370, Length=375, Percent_Identity=26.4, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320016, Length=227, Percent_Identity=29.9559471365639, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24638766, Length=349, Percent_Identity=29.7994269340974, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI28574560, Length=327, Percent_Identity=31.8042813455657, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24658560, Length=374, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24583988, Length=364, Percent_Identity=27.4725274725275, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583986, Length=364, Percent_Identity=27.4725274725275, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583984, Length=364, Percent_Identity=27.4725274725275, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583992, Length=306, Percent_Identity=28.4313725490196, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI281361666, Length=356, Percent_Identity=26.4044943820225, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24646341, Length=356, Percent_Identity=26.4044943820225, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17136796, Length=356, Percent_Identity=26.4044943820225, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI20129479, Length=273, Percent_Identity=28.9377289377289, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): SPAL_SALTI (P0A1C0)

Other databases:

- EMBL:   AL627276
- EMBL:   AE014613
- RefSeq:   NP_457288.1
- RefSeq:   NP_806497.1
- ProteinModelPortal:   P0A1C0
- SMR:   P0A1C0
- PRIDE:   P0A1C0
- GeneID:   1068850
- GeneID:   1249323
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2796
- KEGG:   sty:STY3017
- HOGENOM:   HBG565875
- OMA:   PLYSERA
- ProtClustDB:   PRK08149
- BioCyc:   SENT209261:T2796-MONOMER
- BioCyc:   SENT220341:STY3017-MONOMER
- BRENDA:   3.6.3.14
- GO:   GO:0005737
- GO:   GO:0009405
- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR004100
- InterPro:   IPR005714
- SMART:   SM00382
- TIGRFAMs:   TIGR01026

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N

EC number: =3.6.3.14

Molecular weight: Translated: 47611; Mature: 47611

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTV
CCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LSLIGNAQGLSRDVVLYPTGRALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVID
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEE
VAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFASAGCGKTMLMHMLIEQTEADV
ECCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCE
FVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ
EEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
GKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAF
CCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
YTVLLESEEEADPMADEIRSILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
EQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMRDSLKAWLCQPVAQYSSFDDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
LSGMNAFADQN
HHHHHHHCCCC
>Mature Secondary Structure
MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTV
CCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LSLIGNAQGLSRDVVLYPTGRALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVID
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEE
VAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFASAGCGKTMLMHMLIEQTEADV
ECCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCE
FVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ
EEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
GKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAF
CCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
YTVLLESEEEADPMADEIRSILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
EQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMRDSLKAWLCQPVAQYSSFDDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
LSGMNAFADQN
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504