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The map label for this gene is amyA [H]

Identifier: 194735074

GI number: 194735074

Start: 2028839

End: 2030323

Strand: Direct

Name: amyA [H]

Synonym: SeSA_A2120

Alternate gene names: 194735074

Gene position: 2028839-2030323 (Clockwise)

Preceding gene: 194734713

Following gene: 194735227

Centisome position: 43.08

GC content: 51.38

Gene sequence:

>1485_bases
ATGAAAAACCCCACGTTATTGCAGTACTTCCACTGGTATTATCCCGACGGCGGTAAACTCTGGCCTGAGCTGGCGGAACG
TGCTGATGGGCTGAATGATATCGGTATCAATATGGTCTGGCTACCGCCCGCCTGTAAAGGCGCCTCCGGCGGCTATTCCG
TAGGCTATGATAGCTACGACCTGTTTGACCTCGGCGAATTTGACCAAAAAGGAACTATCGCGACAAAGTACGGCGATAAA
CGCCAGTTACTGACGGCGATAGACGCGCTCAAAAAAAATAATATTGCGGTGCTGCTCGACGTCGTCGTGAATCACAAAAT
GGGGGCAGACGAAAAAGAACGTATTCGCGTTCAGCGCGTGAATCAGGATGACCGCACGCAAATCGATGACAACATCATTG
AATGCGAAGGCTGGACGCGCTACACCTTCCCTGCCCGCGCGGGCCAGTATTCCAACTTTATTTGGGACTATCACTGTTTC
AGCGGCATTGATCACATCGAGAATCCCGACGAAGACGGCATTTTTAAGATCGTCAATGACTATACCGGCGATGGCTGGAA
CGATCAGGTTGATGATGAGATGGGTAATTTCGACTATCTGATGGGTGAAAATATCGATTTTCGCAATCACGCGGTTACGG
AAGAGATTAAATATTGGGCTCGTTGGGTCATGGAACAAACCCACTGTGACGGCTTTCGCCTGGACGCGGTAAAACATATA
CCCGCCTGGTTTTATAAAGAATGGATTGAGCATGTACAGGCGGTTGCACCAAAACCGCTGTTTATTGTCGCAGAATACTG
GTCGCATGAAGTAGATAAACTGCAAACGTACATCGATCAGGTCGACGGGAAAACCATGCTGTTCGACGCGCCGTTGCAGA
TGAAATTTCACGAGGCCTCGCGCCAGGGCGCGGAGTATGACATGCGCCACATTTTCACCGGCACGCTGGTAGAAGCCGAC
CCTTTTCATGCGGTGACGCTGGTCGCTAACCACGATACACAACCGTTACAGGCGCTGGAAGCGCCTGTAGAACCCTGGTT
CAAACCATTGGCCTATGCGCTGATCCTGCTGCGTGAAAACGGCGTGCCGTCGGTGTTTTATCCCGATTTATACGGCGCCA
GCTATGAGGATAGCGGCGAAAATGGCGAGACCTGTCGGGTCGACATGCCGGTGATTAACCAACTGGATCGGCTGATCCTC
GCTCGTCAGCGTTTTGCGCACGGTATACAAACTCTCTTTTTCGATCATCCTAACTGTATCGCCTTTAGTCGCAGCGGTAC
TGAAGAGAATCCAGGCTGTGTGGTCATACTTTCCAATGGCGACGACGGTGAAAAAACCCTCCTGCTCGGCGACAATTACG
CTAACAAGACCTGGCGTGATTTTCTGGGAAACCGCAGTGAGCATGTTGTAACTAATGATCAAGGCGAAGCGACGTTCTTC
TGCAACGCAGGCAGCGTCAGCGTGTGGGTCATTGAGGACGTGTGA

Upstream 100 bases:

>100_bases
TCGTGCCAGATGCGCCTGGCGCCTCATAATATTTTCCCGTCTCGTATGAAAATTCTTCCATACTCCAGAGGTCGGCTAAA
CGACTTCTGGAGCACGGAAG

Downstream 100 bases:

>100_bases
TGAGCGTTCCCGGCGGCAGAGCGCTGCCGGGAAGCGAAATTACGGCAGCGGCGTCGGCAGCGGCGTTTTTTCTAACGCGG
CGGCACACTCCGGCGTCGGA

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNPTLLQYFHWYYPDGGKLWPELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVILSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV

Sequences:

>Translated_494_residues
MKNPTLLQYFHWYYPDGGKLWPELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVILSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV
>Mature_494_residues
MKNPTLLQYFHWYYPDGGKLWPELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVILSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=88.8663967611336, Blast_Score=926, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56479; Mature: 56479

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPTLLQYFHWYYPDGGKLWPELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYD
CCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVILSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC
>Mature Secondary Structure
MKNPTLLQYFHWYYPDGGKLWPELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYD
CCCCCEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVILSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 11677609; 1527488; 8371104 [H]