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The map label for this gene is truC [H]

Identifier: 194734776

GI number: 194734776

Start: 3019820

End: 3020602

Strand: Reverse

Name: truC [H]

Synonym: SeSA_A3124

Alternate gene names: 194734776

Gene position: 3020602-3019820 (Counterclockwise)

Preceding gene: 194735471

Following gene: 194735907

Centisome position: 64.14

GC content: 57.98

Gene sequence:

>783_bases
ATGCTGGAGATCCTCTATCAAGACCAGTGGCTGGTTGCCGTGAATAAACCCGCAGGCTGGCTTGTTCACCGGAGCTGGCT
GGATCGCGACGAAAAAGTCGTGGTCATGCAAACGGTGCGCGACCAAATCGGCCAGCATGTTTTTACCGCCCACCGTCTCG
ACAGACCCACGTCGGGCGTACTGTTAATGGGGCTGTCCAGCGAAGCGGGACGCCGTCTGGCGCAGCAGTTCGAGCAGCAC
CACATCCGTAAACGCTACCATGCCATTGTGCGCGGCTGGCTGATGGATGACGCAGTACTGGATTATCCGCTGGTGGAAGA
GCGCGATAAAATTGCTGATAAATTCGCCCGCGAGGATAAAGCGCCCCAGCCGGCTGTAACGCAGTATCGCGGACTGGCGA
CGGTCGAAATGGCGGTGCCGACCGGACGTTATCCCACCACGCGTTATGGTCTGGTTGAGCTGGAACCAAAAACGGGACGC
AAGCACCAGCTTCGTCGTCATCTGGCGCATCTGCGTCATCCTATCATCGGCGACAGCAAGCATGGCGATTTGCGACAAAA
CCGCAGCGCCGCAGAGCATTTTGCCTGTCGTCGCCTGATGCTTCATGCCAGTCGGCTTGAACTGACGCACCCCTTCACCG
GACAGCCGTTGATGATTCAGGCCGGGTTGGATGAAACCTGGATGCAGGCGCTGACACAGTTTGGCTGGCGTGGACTTCTC
CCTGACAATGAAAGGGTTGAGTTTACGGCGGCGTCCCGGCAGGATGAGACTCATCAGGCATAA

Upstream 100 bases:

>100_bases
CTTATTATGAAATGGCGCTGGCGGCGGATCATCCGCAGAGGGAGGCGATCCTGGCGGTTTTGCAGGATCTGGATGCGCTA
TTCGTCCGCGATAAATCCTG

Downstream 100 bases:

>100_bases
TTCAGGGAGATACGCATAATGGCGGAAATTGGTATTTTTGTCGGGACCATGTATGGCAACTCACTGCTGGTGGCGGAAGA
AGCGGAAGCGATCCTCGCCA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLEILYQDQWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLMDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLMIQAGLDETWMQALTQFGWRGLL
PDNERVEFTAASRQDETHQA

Sequences:

>Translated_260_residues
MLEILYQDQWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLMDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLMIQAGLDETWMQALTQFGWRGLL
PDNERVEFTAASRQDETHQA
>Mature_260_residues
MLEILYQDQWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLMDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLMIQAGLDETWMQALTQFGWRGLL
PDNERVEFTAASRQDETHQA

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI221316663, Length=204, Percent_Identity=30.3921568627451, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789155, Length=255, Percent_Identity=89.4117647058824, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=31.3304721030043, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786244, Length=225, Percent_Identity=34.6666666666667, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788946, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324506, Length=193, Percent_Identity=25.9067357512953, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583449, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583447, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583445, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921090, Length=187, Percent_Identity=32.0855614973262, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 30080; Mature: 30080

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEILYQDQWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEEECCEEEEECCCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRRLAQQFEQHHIRKRYHAIVRGWLMDDAVLDYPLVEERDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
APQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEEECCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLMIQAGLDETWMQALTQFGWRGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
PDNERVEFTAASRQDETHQA
CCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MLEILYQDQWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEEECCEEEEECCCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRRLAQQFEQHHIRKRYHAIVRGWLMDDAVLDYPLVEERDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
APQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEEECCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLMIQAGLDETWMQALTQFGWRGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
PDNERVEFTAASRQDETHQA
CCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]