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The map label for this gene is pspE [H]

Identifier: 194734356

GI number: 194734356

Start: 1753648

End: 1753962

Strand: Reverse

Name: pspE [H]

Synonym: SeSA_A1811

Alternate gene names: 194734356

Gene position: 1753962-1753648 (Counterclockwise)

Preceding gene: 194735436

Following gene: 194733955

Centisome position: 37.25

GC content: 44.13

Gene sequence:

>315_bases
ATGTTGAAAAAAGGAATATTTGCGTTAGCGCTATTCGTAGCCATGCCGCTTTATGCCGCAGAATACTGGATAGATGTCCG
CATTCCGGAACAATATCAGCGTGAGCATATTCAGGGCGCGATTAATATCCCATTGAAGGAGATTAAATCTCATATCGAAA
CGGTAGTTCCAGACAGGAACGATACGGTAAAGTTATATTGCAACTCCGGGCGACAGTCGGGCATGGCAAAACAAATGTTG
CTTGATATGGGGTATACGCACGCGATGAATATGGGCGGCATCAGTCGTCTTGATATGCCGAAAGTAAAAAAATAG

Upstream 100 bases:

>100_bases
TAAAATTTCGCAGCGTTACAAATAGCGAGTCTGGTCATAATATTTCGTTAAGCTCTGGCGTATAGTGTGTGCGAAATACA
TTACGTGTCAGGACGGAAGT

Downstream 100 bases:

>100_bases
ATAATCTATTGCTCAATACTCATACTAACCGCGGCGCTTAATGCGCCGTTGTTGATCTCTCCGGGTAGTTGATAATAACG
TTTTTGCACATATCTATAAC

Product: thiosulfate:cyanide sulfurtransferase

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MLKKGIFALALFVAMPLYAAEYWIDVRIPEQYQREHIQGAINIPLKEIKSHIETVVPDRNDTVKLYCNSGRQSGMAKQML
LDMGYTHAMNMGGISRLDMPKVKK

Sequences:

>Translated_104_residues
MLKKGIFALALFVAMPLYAAEYWIDVRIPEQYQREHIQGAINIPLKEIKSHIETVVPDRNDTVKLYCNSGRQSGMAKQML
LDMGYTHAMNMGGISRLDMPKVKK
>Mature_104_residues
MLKKGIFALALFVAMPLYAAEYWIDVRIPEQYQREHIQGAINIPLKEIKSHIETVVPDRNDTVKLYCNSGRQSGMAKQML
LDMGYTHAMNMGGISRLDMPKVKK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=103, Percent_Identity=66.9902912621359, Blast_Score=161, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 11868; Mature: 11868

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
7.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
7.7 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKGIFALALFVAMPLYAAEYWIDVRIPEQYQREHIQGAINIPLKEIKSHIETVVPDRN
CCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTVKLYCNSGRQSGMAKQMLLDMGYTHAMNMGGISRLDMPKVKK
CEEEEEECCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKKGIFALALFVAMPLYAAEYWIDVRIPEQYQREHIQGAINIPLKEIKSHIETVVPDRN
CCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
DTVKLYCNSGRQSGMAKQMLLDMGYTHAMNMGGISRLDMPKVKK
CEEEEEECCCCCCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]