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The map label for this gene is galS [H]

Identifier: 194734346

GI number: 194734346

Start: 2308581

End: 2309603

Strand: Reverse

Name: galS [H]

Synonym: SeSA_A2430

Alternate gene names: 194734346

Gene position: 2309603-2308581 (Counterclockwise)

Preceding gene: 194734996

Following gene: 194734100

Centisome position: 49.05

GC content: 55.91

Gene sequence:

>1023_bases
ATGATCACCATTCGTGATGTAGCGCGCCAGGCTGGCGTGTCTGTAGCGACCGTTTCCCGCGTACTGAATAACAGCGCGTT
GGTCAGTCCCGACACCCGTGACGCCGTTATGCAGGCCGTCACTCTGCTGGGATATCGGCCAAATGCGAATGCGCAAGCGC
TGGCCACCCAGGTGAGCGATACCATCGGCGTCGTGGTCATGGATGTTTCCGATGCCTTTTTCGGTGCTCTGGTGAAAGCC
GTAGATCTGGTCGCGCAGCAGCACCAGAAATATGTTCTCATTGGCAACAGTTATCATGAGGCGGAAAAAGAGCGTCATGC
GATTGAAGTCTTGATCCGTCAGCGTTGTAACGCATTGATTGTTCACTCAAAAGCCTTAACCGATCGCGAGCTGAGCGACT
TTATGGATCAGATCCCCGGCATGGTGCTGATTAACCGTATCGTGCCGGGTTATGCGCATCGTTGTGTCTGTCTCGACAAT
GTGAGCGGCGCCAGAATGGCGACCCGAATGTTGCTGAATAATGGACATCAACGCATCGGCTACCTGGCCTCCAGCCACCG
TATTGAAGATGACGCGATGCGCAGAGAAGGGTGGTTTCACGCGCTGCAAGAGCAGGGGGTTGCTGCGTCGGAGAGCTGGA
TTGGCACCGGCACGCCGGATATGCAGGGCGGCGAGTCGGCAATGGTTGAGTTGTTGGGACGCAATCTGCAACTGACGGCG
GTATTTGCCTATAACGATAACATGGCGGCGGGCGCGCTGACGGCGTTAAAAGATAACGGCATCGCCATTCCCTTGCATCT
GTCTGTCATCGGTTTCGATGATATCCCTATTGCTCGTTATACCGATCCTCAGCTGACTACCGTGCGCTATCCTATTGCTT
CTATGGCGAAAATCGCGACCGAGCTGGCGTTACAGGGGGCCGCAGGCTCCCTGGATATCACGGCGACGCACTGCTTCATG
CCGACACTGGTGCGGCGCCATTCGGTGGCGTGGCGACAGAATGCGGTACTGATCACTAACTGA

Upstream 100 bases:

>100_bases
TGTGATGTTTCTCACGCAGCCCTGGGCAGGGTGCTGACAAAATAGCCGCCTGCTAACGGGGCATTTGTGAGCATTTTTGA
ACTCAAACAGGGCAGTGAAT

Downstream 100 bases:

>100_bases
TTACGCACCGCATGTAACCGTTTTCAATCTGTGAGTAAATTCACAGTTTATTAACATTGTGATAGCTATGATGACAACGT
TTGTCGCACTGTAACTAATG

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWFHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGSLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Sequences:

>Translated_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWFHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGSLDITATHCFM
PTLVRRHSVAWRQNAVLITN
>Mature_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWFHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGSLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=88.5294117647059, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=56.6666666666667, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=36.5269461077844, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=33.2247557003257, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.5315315315315, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=29.2763157894737, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787580, Length=327, Percent_Identity=28.7461773700306, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=29.1907514450867, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=26.0450160771704, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.1187739463602, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=24.1157556270096, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789456, Length=306, Percent_Identity=27.4509803921569, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.4295532646048, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36970; Mature: 36970

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHH
YLASSHRIEDDAMRREGWFHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCHHHHEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGSLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHH
YLASSHRIEDDAMRREGWFHALQEQGVAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCHHHHEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGSLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019 [H]