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The map label for this gene is sapA [H]

Identifier: 194734041

GI number: 194734041

Start: 1756853

End: 1758502

Strand: Direct

Name: sapA [H]

Synonym: SeSA_A1817

Alternate gene names: 194734041

Gene position: 1756853-1758502 (Clockwise)

Preceding gene: 194734642

Following gene: 194735684

Centisome position: 37.31

GC content: 53.82

Gene sequence:

>1650_bases
ATGCGCCTGGTTTTATCGTCTCTGATCGTGATAGCGGGTCTACTGAGTAGTCAGGCTACGGCTGCGGCTGCGCCCGAACA
AACTGCGAGTGCAGATATTCGCGATAGCGGCTTTGTGTATTGTGTTAGCGGGCAGGTCAACACCTTTAATCCGCAAAAAG
CGAGCAGCGGCCTCATCGTCGATACCCTGGCCGCCCAGTTATATGATCGCCTGTTGGATGTCGATCCCTATACTTATCGT
TTAGTCCCAGAGCTGGCAGAAAGCTGGGAAGTGCTGGATAACGGGGCAACGTACCGTTTTCACCTGCGCCGCGACGTTTC
CTTTCAAAAAACCGCCTGGTTTACGCCGACCCGAAAACTCAATGCTGATGATGTTGTCTTTACCTTTCAGCGGATTTTTG
ATCGTCGGCATCCGTGGCATAACATCAACGGCAGTAGCTTCCCCTACTTTGATAGCCTACAGTTCGCCGACAATGTAAAA
AGCGTGCGTAAGCTGGACAATAACACCGTTGAGTTTCGCCTGACGCAGCCAGACGCCTCCTTTTTATGGCATCTGGCTAC
ACACTACGCTTCCGTCATGTCCGCTGAGTACGCCGCGCAGCTTAGCCGAAAAGATCGTCAGGAACTGCTAGACCGCCAAC
CGGTCGGCACCGGGCCTTTCCAGCTTTCGGAGTACCGTGCCGGGCAGTTTATTCGTCTCCAGCGCCACGATGGTTTTTGG
CGCGGCAAACCGCTGATGCCGCAAGTAGTAGTGGATTTAGGCTCCGGCGGTACCGGGCGTTTATCAAAATTACTGACCGG
TGAATGCGATGTTCTGGCCTGGCCCGCCGCCAGCCAGCTAACTATTTTACGCGACGATCCGCGTTTGCGCCTGACGTTGC
GCCCGGGGATGAATATCGCCTATCTGGCCTTTAACACCGATAAGCCGCCGTTGAATAATCCCGCAGTGCGCCATGCGCTG
GCCTTATCGATCAACAACCAGCGCCTGATGCAGTCGATTTATTACGGCACGGCAGAAACCGCAGCCTCCATTTTACCGAG
AGCCTCATGGGCTTACGATAACGATGCCAAAATTACGGAGTACAATCCGGAAAAATCGCGCGAACAGCTAAAAGCGCTGG
GCATTGAGAATCTTACGCTGCATCTCTGGGTGCCGACTAGTTCTCAGGCCTGGAACCCAAGTCCGCTAAAAACGGCGGAG
CTTATTCAGGCGGATATGGCGCAGATTGGCGTAAAAGTGGTCATTGTGCCGGTTGAAGGTCGTTTCCAGGAGGCGCGCCT
GATGGATATGAATCACGATCTGACCTTATCCGGCTGGGCCACGGACAGCAACGATCCGGATAGCTTTTTCAGACCGCTGT
TAAGCTGTGCAGCCATCAATTCGCAAACCAATTTCGCCCACTGGTGTAACCCTGAATTTGACAGCGTGCTGCGTAAAGCA
CTGTCGTCGCAGCAGTTGGCTTCGCGCATAGAAGCGTATGAGGAAGCGCAGAATATCCTGGAGAAAGAGCTGCCGATACT
GCCGCTGGCATCATCACTACGCCTGCAGGCTTACCGCTACGATATTAAAGGGCTGGTGTTAAGCCCGTTCGGCAATGCGT
CTTTTGCCGGCGTCTCCCGCGAAAAACACGAAGAGGTGAAAAAACCATGA

Upstream 100 bases:

>100_bases
GCTAATTGACGACATTTACGCCAGTTATCCACCGACATTTTTACGTGGCGGGCCGAAGTGCGATACACTTTGCAAATTGA
ACTTCAAAAACTTAACTATT

Downstream 100 bases:

>100_bases
TTATCTTCACCCTGCGTCGGTTATTGCTGTTGCTGGTGACGCTATTCTTCCTGACCTTTATCGGCTTTAGCCTGAGCTAT
TTTACGCCGCACGCGCCGCT

Product: peptide transport periplasmic protein SapA

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRLVLSSLIVIAGLLSSQATAAAAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYRFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPEKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQIGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYEEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP

Sequences:

>Translated_549_residues
MRLVLSSLIVIAGLLSSQATAAAAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYRFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPEKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQIGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYEEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP
>Mature_549_residues
MRLVLSSLIVIAGLLSSQATAAAAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIVDTLAAQLYDRLLDVDPYTYR
LVPELAESWEVLDNGATYRFHLRRDVSFQKTAWFTPTRKLNADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVK
SVRKLDNNTVEFRLTQPDASFLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
RGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIAYLAFNTDKPPLNNPAVRHAL
ALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITEYNPEKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAE
LIQADMAQIGVKVVIVPVEGRFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
LSSQQLASRIEAYEEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSREKHEEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=549, Percent_Identity=89.9817850637523, Blast_Score=1005, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=515, Percent_Identity=36.504854368932, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787052, Length=547, Percent_Identity=26.8738574040219, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787762, Length=490, Percent_Identity=23.8775510204082, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=23.7903225806452, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787495, Length=549, Percent_Identity=24.5901639344262, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789397, Length=535, Percent_Identity=24.1121495327103, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=24.2990654205607, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61636; Mature: 61636

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVLSSLIVIAGLLSSQATAAAAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIV
CHHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
DTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRRDVSFQKTAWFTPTRKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
NADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVKSVRKLDNNTVEFRLTQPDAS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
FLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCCCCC
RGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIA
CCCCCCHHHEEECCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEE
YLAFNTDKPPLNNPAVRHALALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITE
EEEEECCCCCCCCHHHHEEEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEE
YNPEKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAELIQADMAQIGVKVVIVPVEG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCC
RFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
CCCHHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LSSQQLASRIEAYEEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCCCCCH
EKHEEVKKP
HHHHHHCCC
>Mature Secondary Structure
MRLVLSSLIVIAGLLSSQATAAAAPEQTASADIRDSGFVYCVSGQVNTFNPQKASSGLIV
CHHHHHHHHHHHHHHHCCCHHHCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
DTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRRDVSFQKTAWFTPTRKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
NADDVVFTFQRIFDRRHPWHNINGSSFPYFDSLQFADNVKSVRKLDNNTVEFRLTQPDAS
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHH
FLWHLATHYASVMSAEYAAQLSRKDRQELLDRQPVGTGPFQLSEYRAGQFIRLQRHDGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEECCCCCC
RGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLTILRDDPRLRLTLRPGMNIA
CCCCCCHHHEEECCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEEEEEECCCCCEE
YLAFNTDKPPLNNPAVRHALALSINNQRLMQSIYYGTAETAASILPRASWAYDNDAKITE
EEEEECCCCCCCCHHHHEEEEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEE
YNPEKSREQLKALGIENLTLHLWVPTSSQAWNPSPLKTAELIQADMAQIGVKVVIVPVEG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCC
RFQEARLMDMNHDLTLSGWATDSNDPDSFFRPLLSCAAINSQTNFAHWCNPEFDSVLRKA
CCCHHHEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LSSQQLASRIEAYEEAQNILEKELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVSR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCCCCCH
EKHEEVKKP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]