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The map label for this gene is rumA [H]

Identifier: 194733918

GI number: 194733918

Start: 3009441

End: 3010736

Strand: Reverse

Name: rumA [H]

Synonym: SeSA_A3116

Alternate gene names: 194733918

Gene position: 3010736-3009441 (Counterclockwise)

Preceding gene: 194738114

Following gene: 194738051

Centisome position: 63.93

GC content: 54.24

Gene sequence:

>1296_bases
ATGGCGCAATTCTACTCTGCAAAACGGCGCGTGACGACGCGTCAGATCATAACCGTTAAAGTCAATGACCTCGATTCATT
TGGTCAGGGCGTAGCCCGTCATAACGGAAAGGCGCTGTTTATCCCCGGTTTATTGCCTGAGGAAAGCGCAGAAGTCATTC
TTACAGAAGATAAAAAGCAATTTGCCCGCGCACGCGTTTCGCGCCGTTTGAATGATAGCCCGGAACGAGAAACGCCGCGC
TGCCCGCATTTTGGCGTCTGTGGCGGTTGTCAGCAGCAGCATGTCAGCATCGCCCTGCAACAACGCAGCAAAAGCGCGGC
GCTGGCGCGTTTGATGAAACACGAGGTGAATGACATCATCGCCGGCGAGCCCTGGGGTTATCGGCGTCGTGCGCGGTTAA
GTCTGAATTGCCTGCCGGATAAACCGCTGCAAATGGGATTTCGCAAAGCGGGTTCCAGCGATATCGTCAATGTCGAACAG
TGCCCTGTTTTGGCGCCCCAGCTTGAAGCATTGCTGCCCCGAATCAGGGCGTGTCTGGCGAGTTTACACGGCACCCGACA
TCTTGGGCATGTTGAGCTGGTACAGGCGGGAAGCGGTACGCTGATGATTCTGCGCCATACCGCGCCATTGAGTGCGGCGG
ATAAAGAAAAACTGGAACGCTTTTCGCATTCTGAGGGATTGTCTCTGTTTCTGGCGCCGTTTAGCGAGATACTGGAAACG
GTATCTGGCGAAGCGCCCTGGTATGATTCGCACGGCTTACGTTTAGCCTTCAGTCCGCGAGACTTTATTCAGGTTAATGA
GGTGGTAAACCAGCAAATGGTCGCACGCGCGCTGGAGTGGCTTGATGTGCGCGCCGAGGATCGTGTCCTGGATCTGTTCT
GCGGAATGGGGAATTTTACGCTGCCGCTGGCAACGCGCGCGGCAAGCGTGGTAGGGGTTGAGGGCGTTCCGGCGCTGGTA
GAAAAAGGCCGTGAAAACGCCATCCGCAATGGTTTACATAATGTGACATTCTTCCATGAGAACCTGGAGGAAGATGTCAC
GAAGCAGCCGTGGGCGAAAAACGGCTTTGACAAAGTCTTACTCGATCCTGCGCGTGCGGGGGCTACAGGAGTGATGCGAC
ATATTATAAAATTAAAACCTATTCGCATTGTTTATGTATCCTGTAACCCGGCGACGCTGGCGCGCGATAGTGAAGCGCTG
GTCAATGCGGGATATGAGGTTACGCGTTTAGCGATGCTCGACATGTTCCCGCACACAGGACATCTGGAATCAATGGTTCT
GTTCGAGCGCATGTAA

Upstream 100 bases:

>100_bases
GGATCAGAATCATCATGCGTGCGCGCAGGCTGTAGTTGGTCATGGAGTTCCGTTATGGGACAATTAGGGTCACACTGTTA
AATTGAGAAAAAGCTGGTCG

Downstream 100 bases:

>100_bases
TGATTACCGGCTTACCGACTTCGGTAGGCCTGGTCCCTTAAGGAGAGGACGATGGTCGCGGTAAGAAGTGCACATATTAA
TAAAGCTGGTGAATTTGATC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVILTEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGEPWGYRRRARLSLNCLPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLAFSPRDFIQVNEVVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVPALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM

Sequences:

>Translated_431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVILTEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGEPWGYRRRARLSLNCLPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLAFSPRDFIQVNEVVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVPALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM
>Mature_430_residues
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVILTEDKKQFARARVSRRLNDSPERETPRC
PHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGEPWGYRRRARLSLNCLPDKPLQMGFRKAGSSDIVNVEQC
PVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILETV
SGEAPWYDSHGLRLAFSPRDFIQVNEVVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVPALVE
KGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALV
NAGYEVTRLAMLDMFPHTGHLESMVLFERM

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=34.2995169082126, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=34.2995169082126, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=84.7222222222222, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1787083, Length=373, Percent_Identity=26.8096514745308, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=33.5164835164835, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24666579, Length=215, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 47915; Mature: 47784

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVILTEDKKQ
CCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEPWGYRRRARLSLNCLPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLEALLPRIRACLA
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLAFSPRDFIQVNEVVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVVGVEGVPALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC
>Mature Secondary Structure 
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVILTEDKKQ
CCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEPWGYRRRARLSLNCLPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLEALLPRIRACLA
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLAFSPRDFIQVNEVVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVVGVEGVPALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA