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The map label for this gene is rpoD [H]

Identifier: 194334634

GI number: 194334634

Start: 2003541

End: 2004440

Strand: Reverse

Name: rpoD [H]

Synonym: Paes_1835

Alternate gene names: 194334634

Gene position: 2004440-2003541 (Counterclockwise)

Preceding gene: 194334635

Following gene: 194334633

Centisome position: 79.77

GC content: 48.67

Gene sequence:

>900_bases
ATGAGGCAACTAAAAATCAGTAAACAGATCACCAATCGTGAGAGTTTATCTCTTGATCGGTATCTGCAGGAGATCGGCAA
ATACGAGCTGCTGACAGCGGAAGATGAGGTCAAACTGACCATGGCGATCAAGGAAGGATATGACATGCCGGTCGATACTC
CTGAGTACAAGCGAGCAAAGAGAGCGCTCGATAAGCTGATCAAGGGAAATCTTCGCTTTGTTGTATCAGTAGCCAAACAG
TATCAGAATCAGGGTTTAACTCTTGGTGATCTTATCAATGAGGGAAACCTCGGCCTGATCAAGGCAGCAAAACGTTTTGA
TGAGACCCGTGGCTTTAAATTTATTTCCTATGCTGTCTGGTGGATCAGGCAGTCAATTCTTCAGGCTCTTGCCGAACAGT
CGAGAATTGTGCGTCTTCCTTTAAACAGGGTAGGGACGCTCAACAAGATCAGCAAGGCATACAGTCAGCTTGAACAGGAG
TTTGAGCGGGATCCCAATACCAAGGAACTTGCCACTGTGCTCGACATGGACTCACAGGATGTTGCCGATACGCTCAAGAT
CGCCGGCCGTCATGTCTCTGTCGATGCCCCTTTTGCCCAGGGAGATGATAACCGTCTGCTCGATGTGCTTCAGCATGACG
GACAGAAACCAGATCATGGACTGACCCGCGATTCTCTTACCCTTGAGGTTGAGCGTTCTCTCTCGGTCCTGGCTCCCCGT
GAAGCTGACGTTATCCGTTCCTATTTCGGGATCGGTATGGAAAACCCGCTTACGCTGGAAGAGATCGGTGAGAAATTCAA
ACTTACCCGTGAAAGAGTCCGTCAGATCAAGGAAAAAGCCATCAGAAGATTGCGCCAGTCAGCCTATAGTAAAGTTCTCA
AGGAGTACATCGGCAGTTAG

Upstream 100 bases:

>100_bases
GATGAAAGGTTTGCTTTTTCATTGTGGGCGAAATTAATATTTTAAGGCCATTTTGGAGTAACAGTGTTCTTTAAGGACGA
AGAATAGATGGAGAGAGTCA

Downstream 100 bases:

>100_bases
TCGCTGCCAGTTGTCATGGGTAATATTCATGTCAAGCCCGGACTGCTCAATGGCGGAGAAGATGGACCCAGGCTGTTGCC
GGGTAAGGATAACAACCGTT

Product: RNA polymerase sigma-32 subunit RpoH

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYELLTAEDEVKLTMAIKEGYDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
FERDPNTKELATVLDMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQHDGQKPDHGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMENPLTLEEIGEKFKLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGS

Sequences:

>Translated_299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYELLTAEDEVKLTMAIKEGYDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
FERDPNTKELATVLDMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQHDGQKPDHGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMENPLTLEEIGEKFKLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGS
>Mature_299_residues
MRQLKISKQITNRESLSLDRYLQEIGKYELLTAEDEVKLTMAIKEGYDMPVDTPEYKRAKRALDKLIKGNLRFVVSVAKQ
YQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVWWIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQE
FERDPNTKELATVLDMDSQDVADTLKIAGRHVSVDAPFAQGDDNRLLDVLQHDGQKPDHGLTRDSLTLEVERSLSVLAPR
EADVIRSYFGIGMENPLTLEEIGEKFKLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=41.0788381742739, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=38.5767790262172, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789871, Length=291, Percent_Identity=26.8041237113402, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29.5, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34241; Mature: 34241

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLKISKQITNRESLSLDRYLQEIGKYELLTAEDEVKLTMAIKEGYDMPVDTPEYKRAK
CCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHH
RALDKLIKGNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVW
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
WIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQEFERDPNTKELATVLDMDSQD
HHHHHHHHHHHHCCCEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHH
VADTLKIAGRHVSVDAPFAQGDDNRLLDVLQHDGQKPDHGLTRDSLTLEVERSLSVLAPR
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCC
EADVIRSYFGIGMENPLTLEEIGEKFKLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQLKISKQITNRESLSLDRYLQEIGKYELLTAEDEVKLTMAIKEGYDMPVDTPEYKRAK
CCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHH
RALDKLIKGNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAKRFDETRGFKFISYAVW
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHH
WIRQSILQALAEQSRIVRLPLNRVGTLNKISKAYSQLEQEFERDPNTKELATVLDMDSQD
HHHHHHHHHHHHCCCEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHH
VADTLKIAGRHVSVDAPFAQGDDNRLLDVLQHDGQKPDHGLTRDSLTLEVERSLSVLAPR
HHHHHHHCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCC
EADVIRSYFGIGMENPLTLEEIGEKFKLTRERVRQIKEKAIRRLRQSAYSKVLKEYIGS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]