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The map label for this gene is gltX

Identifier: 194334583

GI number: 194334583

Start: 1946732

End: 1948243

Strand: Reverse

Name: gltX

Synonym: Paes_1783

Alternate gene names: 194334583

Gene position: 1948243-1946732 (Counterclockwise)

Preceding gene: 194334587

Following gene: 194334581

Centisome position: 77.53

GC content: 51.26

Gene sequence:

>1512_bases
ATGCAAACAACGCTTGGCCCCAGGGTTAGAACCAGGTTCGCACCATCTCCAACAGGATATCTTCACGTCGGCGGCTTGCG
CACAGCGCTGTATAATTATCTCTATGCAAAGAAAGTCGGAGGTGACTTCATTGTCAGGATCGAAGACACAGACCAGGCCA
GAAAAGTCGAAGGTGCAGATCAGAGTTTGCTCAAAACACTTGAAGAATCAGGGATCGTTGCTGACGAAAGTATTCTTCAC
GGCGGAAATTTCGGTCCCTACATGCAGTCAGAGCGCCTTGACACCTATGCACAGTACTGCCGTCAGCTGCTCGATCAAAA
CAACGCCTATTACTGTTTCTCAACGGCAGAAGAGCTCGACGAAAACCGCAAGCTTCAGATGAAGCAGGGTATTCAGCCCA
AATACAACCGCAAGTGGCTGCCTGAAACCATGGGCGGCAACATGCCTCAGAGTGAAATTCAGAAAAAACTTGATGAAGGA
GCCCCTTACGTTATCCGTATGAAGGTTCCCGATTACATCTCCATCATGTTCGAAGATATCGTTCGCGGTCCGGTTGAATT
CGATTCGGCCACTGTCGACGACCAGGTCCTCATGAAATCCGATGGTTTCCCGACCTATCACTTCGCCAGCGTCATCGACG
ACCATCTCATGGAAATCACCCATATTATCCGCGGTGAAGAGTGGCTTTCGTCCATGCCGAAACATCTTCTTCTCTATGAA
TTCTTCGGATGGGAAGCTCCCAGGTTCGCTCATCTGCCGCTCCTCCTCAATCCGGATCGCTCAAAACTCAGTAAGCGCCA
GGGCGACGTTGCGGTTGAAGACTTCATCGCCAAAGGCTACAGCCCCGACGCCATTCTTAACTTCGTCGCCCTGCTCGGGT
GGAACGAAGGCGAAGGCTCGGAACAGGAGATCTACTCCCTCGAAGAGCTCGTGCAGAAATTCTCGCTCGAACGCGTTGGT
AAAGCGGGAGCTGTTTTCAACATCGACAAGCTTGACTGGATTGAAAAACAGTATATCAAAGCCCGTCCTGTCGACCAGAT
TATCCAGGCAATCAAGCCGCTTCTCCAGAAAGAACTCGCTGAAAAGTCGACCGACATGGATGTTGCGCGCATCAGCAGCG
ACGAATACCTGACACAGGTCATCGACCTCATGCGCGAACGCGTCAACTTCGAAAACGAGTTTATCACCTTCTCGAACTAC
TTCTACTTCGATCCCGAAACCTACGACGAAGCCGCAGTAGCAAAACGCTGGCAGGACGATACCAACGAGGTGCTTGCCGA
ATTCATCACCGAACTCGAAGAAAGCGATGATTTCAGTGCCGACAACATTGAAGCCATGCTTAAGGCATTCGTCGCACCAA
AAGGCAAAAAACCCGCATTTCTCATACACCCGCTCAGGATCCTCAGCTCAGGCGTCGGCTTCGGCCCAAGTCTCTACCAC
ATGCTCGAAGTCCTCGGCAAACAAACCGTCATCCGCCGCCTCAAAAAAGGCATCGAACGCGTAACAGTGTAA

Upstream 100 bases:

>100_bases
CCATAACAAACAACAACCCTGCCGCTATGAATCCAACCGACTTTTTTTTAAATTCCAAGCCATTTATAAACCGCAGAAAA
CAGCAAATATTCAAGGTCTT

Downstream 100 bases:

>100_bases
AAGAGTGTCGGAGGGGGGGAAGAGGTGATTGGGCCGACAGGCGCCAATCGCCCCGTCGCCCTTCACTCCTCCGCGCCGAC
AGAAAGAAGGGGACGTTCTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MQTTLGPRVRTRFAPSPTGYLHVGGLRTALYNYLYAKKVGGDFIVRIEDTDQARKVEGADQSLLKTLEESGIVADESILH
GGNFGPYMQSERLDTYAQYCRQLLDQNNAYYCFSTAEELDENRKLQMKQGIQPKYNRKWLPETMGGNMPQSEIQKKLDEG
APYVIRMKVPDYISIMFEDIVRGPVEFDSATVDDQVLMKSDGFPTYHFASVIDDHLMEITHIIRGEEWLSSMPKHLLLYE
FFGWEAPRFAHLPLLLNPDRSKLSKRQGDVAVEDFIAKGYSPDAILNFVALLGWNEGEGSEQEIYSLEELVQKFSLERVG
KAGAVFNIDKLDWIEKQYIKARPVDQIIQAIKPLLQKELAEKSTDMDVARISSDEYLTQVIDLMRERVNFENEFITFSNY
FYFDPETYDEAAVAKRWQDDTNEVLAEFITELEESDDFSADNIEAMLKAFVAPKGKKPAFLIHPLRILSSGVGFGPSLYH
MLEVLGKQTVIRRLKKGIERVTV

Sequences:

>Translated_503_residues
MQTTLGPRVRTRFAPSPTGYLHVGGLRTALYNYLYAKKVGGDFIVRIEDTDQARKVEGADQSLLKTLEESGIVADESILH
GGNFGPYMQSERLDTYAQYCRQLLDQNNAYYCFSTAEELDENRKLQMKQGIQPKYNRKWLPETMGGNMPQSEIQKKLDEG
APYVIRMKVPDYISIMFEDIVRGPVEFDSATVDDQVLMKSDGFPTYHFASVIDDHLMEITHIIRGEEWLSSMPKHLLLYE
FFGWEAPRFAHLPLLLNPDRSKLSKRQGDVAVEDFIAKGYSPDAILNFVALLGWNEGEGSEQEIYSLEELVQKFSLERVG
KAGAVFNIDKLDWIEKQYIKARPVDQIIQAIKPLLQKELAEKSTDMDVARISSDEYLTQVIDLMRERVNFENEFITFSNY
FYFDPETYDEAAVAKRWQDDTNEVLAEFITELEESDDFSADNIEAMLKAFVAPKGKKPAFLIHPLRILSSGVGFGPSLYH
MLEVLGKQTVIRRLKKGIERVTV
>Mature_503_residues
MQTTLGPRVRTRFAPSPTGYLHVGGLRTALYNYLYAKKVGGDFIVRIEDTDQARKVEGADQSLLKTLEESGIVADESILH
GGNFGPYMQSERLDTYAQYCRQLLDQNNAYYCFSTAEELDENRKLQMKQGIQPKYNRKWLPETMGGNMPQSEIQKKLDEG
APYVIRMKVPDYISIMFEDIVRGPVEFDSATVDDQVLMKSDGFPTYHFASVIDDHLMEITHIIRGEEWLSSMPKHLLLYE
FFGWEAPRFAHLPLLLNPDRSKLSKRQGDVAVEDFIAKGYSPDAILNFVALLGWNEGEGSEQEIYSLEELVQKFSLERVG
KAGAVFNIDKLDWIEKQYIKARPVDQIIQAIKPLLQKELAEKSTDMDVARISSDEYLTQVIDLMRERVNFENEFITFSNY
FYFDPETYDEAAVAKRWQDDTNEVLAEFITELEESDDFSADNIEAMLKAFVAPKGKKPAFLIHPLRILSSGVGFGPSLYH
MLEVLGKQTVIRRLKKGIERVTV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=510, Percent_Identity=37.6470588235294, Blast_Score=308, Evalue=8e-84,
Organism=Homo sapiens, GI62241042, Length=280, Percent_Identity=24.6428571428571, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788743, Length=492, Percent_Identity=33.5365853658537, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI226510925, Length=303, Percent_Identity=32.013201320132, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=494, Percent_Identity=35.2226720647773, Blast_Score=277, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=34.5849802371541, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321192, Length=266, Percent_Identity=26.6917293233083, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=502, Percent_Identity=38.0478087649402, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24649466, Length=223, Percent_Identity=27.8026905829596, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PROA2 (B4S404)

Other databases:

- EMBL:   CP001108
- RefSeq:   YP_002016443.1
- ProteinModelPortal:   B4S404
- SMR:   B4S404
- GeneID:   6458845
- GenomeReviews:   CP001108_GR
- KEGG:   paa:Paes_1783
- HOGENOM:   HBG628189
- OMA:   CSSIVEL
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57532; Mature: 57532

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLGPRVRTRFAPSPTGYLHVGGLRTALYNYLYAKKVGGDFIVRIEDTDQARKVEGAD
CCCCCCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCH
QSLLKTLEESGIVADESILHGGNFGPYMQSERLDTYAQYCRQLLDQNNAYYCFSTAEELD
HHHHHHHHHCCCEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
ENRKLQMKQGIQPKYNRKWLPETMGGNMPQSEIQKKLDEGAPYVIRMKVPDYISIMFEDI
CCCCCHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
VRGPVEFDSATVDDQVLMKSDGFPTYHFASVIDDHLMEITHIIRGEEWLSSMPKHLLLYE
HCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHH
FFGWEAPRFAHLPLLLNPDRSKLSKRQGDVAVEDFIAKGYSPDAILNFVALLGWNEGEGS
HHCCCCCCEECCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
EQEIYSLEELVQKFSLERVGKAGAVFNIDKLDWIEKQYIKARPVDQIIQAIKPLLQKELA
HHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EKSTDMDVARISSDEYLTQVIDLMRERVNFENEFITFSNYFYFDPETYDEAAVAKRWQDD
HHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCCHHHHHHHHHHCCC
TNEVLAEFITELEESDDFSADNIEAMLKAFVAPKGKKPAFLIHPLRILSSGVGFGPSLYH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCHHHHH
MLEVLGKQTVIRRLKKGIERVTV
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTTLGPRVRTRFAPSPTGYLHVGGLRTALYNYLYAKKVGGDFIVRIEDTDQARKVEGAD
CCCCCCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCH
QSLLKTLEESGIVADESILHGGNFGPYMQSERLDTYAQYCRQLLDQNNAYYCFSTAEELD
HHHHHHHHHCCCEECHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHC
ENRKLQMKQGIQPKYNRKWLPETMGGNMPQSEIQKKLDEGAPYVIRMKVPDYISIMFEDI
CCCCCHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
VRGPVEFDSATVDDQVLMKSDGFPTYHFASVIDDHLMEITHIIRGEEWLSSMPKHLLLYE
HCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHH
FFGWEAPRFAHLPLLLNPDRSKLSKRQGDVAVEDFIAKGYSPDAILNFVALLGWNEGEGS
HHCCCCCCEECCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
EQEIYSLEELVQKFSLERVGKAGAVFNIDKLDWIEKQYIKARPVDQIIQAIKPLLQKELA
HHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EKSTDMDVARISSDEYLTQVIDLMRERVNFENEFITFSNYFYFDPETYDEAAVAKRWQDD
HHCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCCHHHHHHHHHHCCC
TNEVLAEFITELEESDDFSADNIEAMLKAFVAPKGKKPAFLIHPLRILSSGVGFGPSLYH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCHHHHH
MLEVLGKQTVIRRLKKGIERVTV
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA