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The map label for this gene is metK [H]

Identifier: 194333641

GI number: 194333641

Start: 885090

End: 886307

Strand: Reverse

Name: metK [H]

Synonym: Paes_0808

Alternate gene names: 194333641

Gene position: 886307-885090 (Counterclockwise)

Preceding gene: 194333642

Following gene: 194333640

Centisome position: 35.27

GC content: 51.48

Gene sequence:

>1218_bases
ATGAAGATGGCAAAGTCAAGGTACTTCTTTACCTCAGAGTCTGTATCAGAAGGACATCCCGACAAAGTTTCAGACCAGAT
CTCCGATGCGATCCTGGATGAATTTCTCCGTCAGGACCCGGATTCCCGTGTCGCTTGTGAAACATTTGTCACAACTGGTC
AGGTCATCGTCGGTGGCGAAGTGACGACAAAAGGCATTGTTGACGTTCAGAAGCTGGCAAGAAAAGCGATCGAAAAAATC
GGTTACAACAAAGGCGAGTACATGTTCGAGGCTCAGTCCTGTGGCGTCCTTTCCGCCCTGCACAGCCAGTCACCGGATAT
CAACCGTGGCGTTGACCGTAAAGAAGAGATCGAAGACGAATTCGACAGAGTCGGTGCCGGCGACCAGGGAATGATGTTCG
GTTATGCCTGTACCGAAACTCCGGAGCTCATGCCTGCCGCTATTCAGTACGCACACGCTCTGGTAGAAAAACTTGCCGCA
ATCCGCAAGGAAGGCAAGATCATGACCTACCTCCGTCCTGACTCGAAAAGCCAGGTCACCCTTGAATACGTTGATGACGT
CGCCAAACGAGTCGATGCCGTTGTCGTCTCAACCCAGCACGATCCTGAACCGGAAGGCTGCAGTGAAGCCGAATGGCAGG
AAATCATCAAAAAAGATATCATTGACAATGTCATCAGCAAGGTTATTCCTGCTGACATGATCGATGAAAACACCAAGCTT
CACATCAACCCGACCGGCCGCTTTGAAATCGGTGGGCCACACGGCGATACCGGCCTTACCGGTCGCAAAATCATTGTCGA
TACATACGGCGGTGCTGCTCCTCACGGCGGCGGTGCATTCAGCGGCAAAGACCCGTCCAAAGTTGACCGCAGCGCAGCCT
ACGCATCTCGCCATGTCGCAAAAAACATTGTTGCTGCAGGACTGGCAGACAAATGTACCGTTCAGGTCTCCTACGCCATT
GGTGTCGCACGTCCTGTATCGATCTACATCAACACCCATGGCACGGCAATCAATGGGCTCAGCGATGCAGAGATTCAGGA
AAAAGCCGAAAAAATCTTCGATCTCAGACCGGCTGCCATCATCAAGCACTTCAGCCTCAATAAACCCGAAGGCTGGTGTT
ATCAGGATACCGCAGCATACGGTCACTTCGGACGCGATATCTTCCCATGGGAAAAAACCGATAAGGTTGAAGAACTCAAA
AAAGCCTTCAACCTCTGA

Upstream 100 bases:

>100_bases
TTCCGTGTCCATTCTCTCCAAGTCATTCCAGCACGAAAACCCATTGAAGACAAGCAGGGCTGCCTGTGCAGTGACTGTTG
TTCAGTAAACCATAAAATCA

Downstream 100 bases:

>100_bases
TACGATATTACTTAAAGACACAAAACAAAGACTATGATGACTACTGACACTGCAGTCCTCGACTACAAAGTGGCAGATAT
CTCGCTGGCTGAATGGGGCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKMAKSRYFFTSESVSEGHPDKVSDQISDAILDEFLRQDPDSRVACETFVTTGQVIVGGEVTTKGIVDVQKLARKAIEKI
GYNKGEYMFEAQSCGVLSALHSQSPDINRGVDRKEEIEDEFDRVGAGDQGMMFGYACTETPELMPAAIQYAHALVEKLAA
IRKEGKIMTYLRPDSKSQVTLEYVDDVAKRVDAVVVSTQHDPEPEGCSEAEWQEIIKKDIIDNVISKVIPADMIDENTKL
HINPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAI
GVARPVSIYINTHGTAINGLSDAEIQEKAEKIFDLRPAAIIKHFSLNKPEGWCYQDTAAYGHFGRDIFPWEKTDKVEELK
KAFNL

Sequences:

>Translated_405_residues
MKMAKSRYFFTSESVSEGHPDKVSDQISDAILDEFLRQDPDSRVACETFVTTGQVIVGGEVTTKGIVDVQKLARKAIEKI
GYNKGEYMFEAQSCGVLSALHSQSPDINRGVDRKEEIEDEFDRVGAGDQGMMFGYACTETPELMPAAIQYAHALVEKLAA
IRKEGKIMTYLRPDSKSQVTLEYVDDVAKRVDAVVVSTQHDPEPEGCSEAEWQEIIKKDIIDNVISKVIPADMIDENTKL
HINPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAI
GVARPVSIYINTHGTAINGLSDAEIQEKAEKIFDLRPAAIIKHFSLNKPEGWCYQDTAAYGHFGRDIFPWEKTDKVEELK
KAFNL
>Mature_405_residues
MKMAKSRYFFTSESVSEGHPDKVSDQISDAILDEFLRQDPDSRVACETFVTTGQVIVGGEVTTKGIVDVQKLARKAIEKI
GYNKGEYMFEAQSCGVLSALHSQSPDINRGVDRKEEIEDEFDRVGAGDQGMMFGYACTETPELMPAAIQYAHALVEKLAA
IRKEGKIMTYLRPDSKSQVTLEYVDDVAKRVDAVVVSTQHDPEPEGCSEAEWQEIIKKDIIDNVISKVIPADMIDENTKL
HINPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVAKNIVAAGLADKCTVQVSYAI
GVARPVSIYINTHGTAINGLSDAEIQEKAEKIFDLRPAAIIKHFSLNKPEGWCYQDTAAYGHFGRDIFPWEKTDKVEELK
KAFNL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=396, Percent_Identity=53.5353535353535, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI4557737, Length=400, Percent_Identity=50.75, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1789311, Length=400, Percent_Identity=56.5, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=51.8796992481203, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538494, Length=407, Percent_Identity=51.5970515970516, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=52.0304568527919, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=52.1739130434783, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565905, Length=407, Percent_Identity=46.9287469287469, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=50.1457725947522, Blast_Score=310, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=49.7126436781609, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI32565903, Length=329, Percent_Identity=49.8480243161094, Blast_Score=280, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=58.0246913580247, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=60, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=400, Percent_Identity=54.25, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323209, Length=389, Percent_Identity=53.7275064267352, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580529, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580537, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580533, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580535, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580531, Length=396, Percent_Identity=51.7676767676768, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=51.5228426395939, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=51.5228426395939, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=50.6561679790026, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=50.8196721311475, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44376; Mature: 44376

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAKSRYFFTSESVSEGHPDKVSDQISDAILDEFLRQDPDSRVACETFVTTGQVIVGGE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCC
VTTKGIVDVQKLARKAIEKIGYNKGEYMFEAQSCGVLSALHSQSPDINRGVDRKEEIEDE
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHCCCCCCCCCCCHHHHHHHH
FDRVGAGDQGMMFGYACTETPELMPAAIQYAHALVEKLAAIRKEGKIMTYLRPDSKSQVT
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
LEYVDDVAKRVDAVVVSTQHDPEPEGCSEAEWQEIIKKDIIDNVISKVIPADMIDENTKL
HHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEE
HINPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVA
EECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KNIVAAGLADKCTVQVSYAIGVARPVSIYINTHGTAINGLSDAEIQEKAEKIFDLRPAAI
HHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCHHHH
IKHFSLNKPEGWCYQDTAAYGHFGRDIFPWEKTDKVEELKKAFNL
HHHHCCCCCCCCEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKMAKSRYFFTSESVSEGHPDKVSDQISDAILDEFLRQDPDSRVACETFVTTGQVIVGGE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCC
VTTKGIVDVQKLARKAIEKIGYNKGEYMFEAQSCGVLSALHSQSPDINRGVDRKEEIEDE
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHCCCCCCCCCCCHHHHHHHH
FDRVGAGDQGMMFGYACTETPELMPAAIQYAHALVEKLAAIRKEGKIMTYLRPDSKSQVT
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
LEYVDDVAKRVDAVVVSTQHDPEPEGCSEAEWQEIIKKDIIDNVISKVIPADMIDENTKL
HHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEE
HINPTGRFEIGGPHGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYASRHVA
EECCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KNIVAAGLADKCTVQVSYAIGVARPVSIYINTHGTAINGLSDAEIQEKAEKIFDLRPAAI
HHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCHHHH
IKHFSLNKPEGWCYQDTAAYGHFGRDIFPWEKTDKVEELKKAFNL
HHHHCCCCCCCCEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA