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The map label for this gene is bcr [H]

Identifier: 194333522

GI number: 194333522

Start: 731711

End: 732907

Strand: Reverse

Name: bcr [H]

Synonym: Paes_0688

Alternate gene names: 194333522

Gene position: 732907-731711 (Counterclockwise)

Preceding gene: 194333523

Following gene: 194333521

Centisome position: 29.17

GC content: 53.22

Gene sequence:

>1197_bases
ATGAACGCAGCAGTACCCTCCAGAGAAAAAGGTTTTGCGGAATTTATTGTCCTTATCGCCATGATGATGTCACTCGTAGC
GCTCTCCATCGACACAATGCTGCCTGCGCTGCCGGCAATCATCAACGACCTGAATGTAACAAAGCCGAACTCCGGACAAC
TGGTGCTCTCGACACTGTTTCTCGGCATGTCTGCAGGACAGATCGTTTACGGACCGATTTCCGACACCATCGGACGAAAA
CCAGCTATCTCCATCGGATTCGGCATCTTTATCGCCGGCACACTGCTCTGTCTGAACGCGACCACATTTTCGGCTATGCT
CGGTGGACGCTTCCTGCAGGGCCTGGGGGCTGCGGCTCCAAGAATTATCACCCTCGCTCTTGTCCGGGACAAATACGAAG
GTGCCGAAATGGCACGAATCATGTCCTTCGTTATGACCATCTTCATACTGGTGCCCATCCTTGCTCCGTTTACCGGACAG
GTTATCATGAGCATTGCCGGATGGCGGGGAATTTTCGCCGGTTTTCTCTTCATGGCAACGGGGATTCTCTGCTGGTTTCT
CTGGCGTCAGCCTGAAACGCTCATCAGTGAAAAACGCCATCCATTCTCCCTGGGGCGCATACAGCAGAGTCTGCGGGAAA
TCATCTCGAACCCTCAGTCAATGGTCTATACACTGACTTCCGGCCTGATCTTCGGAGCATTTCTGGGGTACCTCAACACC
GCCCAACAGATCTTTCAGGACCAATACGCGACGGGTGAGATGTTTCCTGTCTATTTCGGCATGCTGGCTCTGCCCTTCGG
CATGGCTACGCTCCTGAACTCAAAACTGGTCAGACACGTCAGCCTTCAGCGAATCGTAGCTCTGGCCATGACAGGTATTG
CCCTCCTGTCAACGATCTTTCTCCTGCCGTTGCTGTTCAGCCATGGCCAACCGCCATTCTGGGCCATTCTCGCCTATCTG
GCCCCGATCTTTTTTGCCATCGGTATTCTCTTCGGCAACCTCAACGCCCTGGCAATGGAACCACTCGGCCATATTGCCGG
AACCGGAGCCGCCACAGTGGGCTCTCTGTCAACCCTGATAGGCATGATAACCGGTACCACTATCGGCCAGTTCTATAACG
GAACCCTTTTTCCGCTGGTTTACGGTTTTCTTGCCTGTACACTTCTCTCTCTACTGATCACACAGATACGAAAGTGA

Upstream 100 bases:

>100_bases
GAACTTGCCCTTGGCAAACATCCTGTTTGCTGAACAGCCCTTCGATGTACACCACCAGATGGCGCAAGCAAATTAACTCC
GCGCAATAACGATACCTCTT

Downstream 100 bases:

>100_bases
CGATCTGCCGTTCTGAAACTCCGTATCAACGGGAAAAACATAAATTGCAGTCTGAAGCGATTGCAGAGTTATGGCGATGG
GTTTACATTGGATACTACGT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNAAVPSREKGFAEFIVLIAMMMSLVALSIDTMLPALPAIINDLNVTKPNSGQLVLSTLFLGMSAGQIVYGPISDTIGRK
PAISIGFGIFIAGTLLCLNATTFSAMLGGRFLQGLGAAAPRIITLALVRDKYEGAEMARIMSFVMTIFILVPILAPFTGQ
VIMSIAGWRGIFAGFLFMATGILCWFLWRQPETLISEKRHPFSLGRIQQSLREIISNPQSMVYTLTSGLIFGAFLGYLNT
AQQIFQDQYATGEMFPVYFGMLALPFGMATLLNSKLVRHVSLQRIVALAMTGIALLSTIFLLPLLFSHGQPPFWAILAYL
APIFFAIGILFGNLNALAMEPLGHIAGTGAATVGSLSTLIGMITGTTIGQFYNGTLFPLVYGFLACTLLSLLITQIRK

Sequences:

>Translated_398_residues
MNAAVPSREKGFAEFIVLIAMMMSLVALSIDTMLPALPAIINDLNVTKPNSGQLVLSTLFLGMSAGQIVYGPISDTIGRK
PAISIGFGIFIAGTLLCLNATTFSAMLGGRFLQGLGAAAPRIITLALVRDKYEGAEMARIMSFVMTIFILVPILAPFTGQ
VIMSIAGWRGIFAGFLFMATGILCWFLWRQPETLISEKRHPFSLGRIQQSLREIISNPQSMVYTLTSGLIFGAFLGYLNT
AQQIFQDQYATGEMFPVYFGMLALPFGMATLLNSKLVRHVSLQRIVALAMTGIALLSTIFLLPLLFSHGQPPFWAILAYL
APIFFAIGILFGNLNALAMEPLGHIAGTGAATVGSLSTLIGMITGTTIGQFYNGTLFPLVYGFLACTLLSLLITQIRK
>Mature_398_residues
MNAAVPSREKGFAEFIVLIAMMMSLVALSIDTMLPALPAIINDLNVTKPNSGQLVLSTLFLGMSAGQIVYGPISDTIGRK
PAISIGFGIFIAGTLLCLNATTFSAMLGGRFLQGLGAAAPRIITLALVRDKYEGAEMARIMSFVMTIFILVPILAPFTGQ
VIMSIAGWRGIFAGFLFMATGILCWFLWRQPETLISEKRHPFSLGRIQQSLREIISNPQSMVYTLTSGLIFGAFLGYLNT
AQQIFQDQYATGEMFPVYFGMLALPFGMATLLNSKLVRHVSLQRIVALAMTGIALLSTIFLLPLLFSHGQPPFWAILAYL
APIFFAIGILFGNLNALAMEPLGHIAGTGAATVGSLSTLIGMITGTTIGQFYNGTLFPLVYGFLACTLLSLLITQIRK

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=387, Percent_Identity=27.1317829457364, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790146, Length=164, Percent_Identity=33.5365853658537, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994889, Length=180, Percent_Identity=34.4444444444444, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082312, Length=340, Percent_Identity=25.2941176470588, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790794, Length=389, Percent_Identity=23.3933161953728, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787065, Length=351, Percent_Identity=26.7806267806268, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42944; Mature: 42944

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]