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The map label for this gene is hldE [H]

Identifier: 194333186

GI number: 194333186

Start: 376494

End: 377012

Strand: Reverse

Name: hldE [H]

Synonym: Paes_0342

Alternate gene names: 194333186

Gene position: 377012-376494 (Counterclockwise)

Preceding gene: 194333191

Following gene: 194333185

Centisome position: 15.0

GC content: 51.45

Gene sequence:

>519_bases
ATGAACACCAGGGAAAAAATACTTACGCAGGATCAGGCGGCAACTATCGTCAAACAATGGCAGCATGAGCATCTCAACGT
CGTCTTTACCAACGGCTGTTTTGACATTCTTCATGCAGGCCATGTCCAATATCTTGAAAACGCCCGTTCGGCAGGCGACC
GTCTGGTTGTCGGCGTCAATACGGATCACTCAGTCCAGCGTCTGAAAGGCCCTGCAAGACCTGTCTGTTCTGAGCATGAC
CGTTGCAGAGTACTTGCTGCGCTCGAATCGGTTGATGCCGTCGTCCTGTTCGAAGAAGACACGCCAATCGAACTGATTGA
AAGGCTACTGCCCGACACGCTTGTCAAAGGTGCCGACTGGGCTCTTGAAGACATTGTCGGTGCCGATGTAGTCCTTGCCA
GAGGTGGTGAAGTGAAAACCATTGCGTTTCTCGACGGCCGTTCAACGACGGGAGTCATTGAAAGAGTCCTGGAATCTTAC
GGTAGAACAGTCTGCCGCGACGAAAAAAAAACACTCTGA

Upstream 100 bases:

>100_bases
TATATGATCGCGGTGACAGGGATTTTATACATAAAAATCCTAAATAATTCATATCTAATAGATACAGGATTTTCAACCCT
CCTCAGGCGCCGGCGAGAAC

Downstream 100 bases:

>100_bases
ACAGGACAACAAACAACCACACCCGGTTCGGACGCATAACGCTCCAGAAGAGAGGTATGACCATCAGCAATATCAGTATC
GAAGAAGCAGTACAACGGTG

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNTREKILTQDQAATIVKQWQHEHLNVVFTNGCFDILHAGHVQYLENARSAGDRLVVGVNTDHSVQRLKGPARPVCSEHD
RCRVLAALESVDAVVLFEEDTPIELIERLLPDTLVKGADWALEDIVGADVVLARGGEVKTIAFLDGRSTTGVIERVLESY
GRTVCRDEKKTL

Sequences:

>Translated_172_residues
MNTREKILTQDQAATIVKQWQHEHLNVVFTNGCFDILHAGHVQYLENARSAGDRLVVGVNTDHSVQRLKGPARPVCSEHD
RCRVLAALESVDAVVLFEEDTPIELIERLLPDTLVKGADWALEDIVGADVVLARGGEVKTIAFLDGRSTTGVIERVLESY
GRTVCRDEKKTL
>Mature_172_residues
MNTREKILTQDQAATIVKQWQHEHLNVVFTNGCFDILHAGHVQYLENARSAGDRLVVGVNTDHSVQRLKGPARPVCSEHD
RCRVLAALESVDAVVLFEEDTPIELIERLLPDTLVKGADWALEDIVGADVVLARGGEVKTIAFLDGRSTTGVIERVLESY
GRTVCRDEKKTL

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505651, Length=133, Percent_Identity=33.8345864661654, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI296841136, Length=133, Percent_Identity=33.8345864661654, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789432, Length=150, Percent_Identity=52.6666666666667, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI193203447, Length=101, Percent_Identity=36.6336633663366, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17509873, Length=101, Percent_Identity=36.6336633663366, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550975, Length=133, Percent_Identity=30.8270676691729, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550973, Length=133, Percent_Identity=30.8270676691729, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550974, Length=133, Percent_Identity=30.8270676691729, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.- [C]

Molecular weight: Translated: 19071; Mature: 19071

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTREKILTQDQAATIVKQWQHEHLNVVFTNGCFDILHAGHVQYLENARSAGDRLVVGVN
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TDHSVQRLKGPARPVCSEHDRCRVLAALESVDAVVLFEEDTPIELIERLLPDTLVKGADW
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCH
ALEDIVGADVVLARGGEVKTIAFLDGRSTTGVIERVLESYGRTVCRDEKKTL
HHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure
MNTREKILTQDQAATIVKQWQHEHLNVVFTNGCFDILHAGHVQYLENARSAGDRLVVGVN
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
TDHSVQRLKGPARPVCSEHDRCRVLAALESVDAVVLFEEDTPIELIERLLPDTLVKGADW
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCH
ALEDIVGADVVLARGGEVKTIAFLDGRSTTGVIERVLESYGRTVCRDEKKTL
HHHHHCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]